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ECM 830 Square Wave Electroporation System

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ECM 830 Square Wave Electroporation System

Versatile electroporation system suitable for applications for gene, drug and protein delivery including: mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant, nuclear transfer, and CRISPR


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Product Item # Price  
ECM 830 Electroporation System, with Safety Dome 45-2052   View Price
ECM 830 Generator Only 45-0662   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-200 45-0666   View Price
ECM 830 Electroporation System, with Safety Stand 45-0661   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-200 45-0664   View Price
ECM 830 Electroporation System Complete, for Cuvettes with Monitoring 45-0667   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-100 45-0663   View Price
ECM 830 High Throughput Electroporation System with Monitoring 45-0668   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-100 45-0665   View Price
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Details

The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer. 

ECM 830 GENERATOR

The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.

The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

The ECM 830 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

FEATURES

  • A wide range of voltages from 5 to 3,000 V
  • Pulse durations from 10 μsec to 999 ms
  • Pre-Pulse Resistance Check, Over-Current Pulse Abort, and Instrument Arc Protection Safety Features
  • Easy to use touchscreen user interface
  • Logs of every pulse delivered for QC and troubleshooting

IN VIVO APPLICATIONS

  • Mammalian cell transfection
  • In vitro, in vivo, ex vivo and in ovo tissue transfection
  • Nuclear transfer
  • Ex plant tissue transfection
  • Plant protoplast transfection
  • siRNA libraries
  • High throughput 25- and 96-well electroporation

ADHERENT CELL TRANSFECTION

The ECM 830 generator coupled with the Petri Pulser electrode or the adherent cell electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser electrode is ideal for 6-well plates and the adherent cell electrodes for 12-well plates.

HIGH THROUGHPUT (HT) ELECTROPORATION (45-0664 and 45-0666)

The High Throughput (HT) 96- and 25-well systems offer the advantage of multi-well electroporation technology for processing multiple samples in seconds. 

This permits a large number of samples to be quickly electroporated using high throughput plate handler technology. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.

The key to the HT System is the combination of the ECM 830 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 830 generator.

MONITORING OPTION

The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each electroporation experiment. 

INCLUDED ITEMS

Item # Product Included Items
45-2052 ECM 830 Electroporation System Complete, with Safety Dome ECM 830 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0661 ECM 830 Electroporation System Complete, with Safety Stand ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0662 ECM 830 Generator Only ECM 830 Generator Only
45-0663 ECM 830 High Throughput Electroporation System, 25-well with HT-100 ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a plate adaptor
45-0664 ECM 830 High Throughput Electroporation System, 25-well with HT-200 ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-200 Plate Handler and a plate adaptor
45-0665 ECM 830 High Throughput Electroporation System, 96-well with HT-100 ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a plate adaptor
 45-0666 ECM 830 High Throughput Electroporation System, 96-well with HT-200 ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-200 Plate Handler and a plate adaptor
45-0667 ECM 830 Electroporation System Complete, for Cuvettes with Monitoring ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0668 ECM 830 High Throughput Electroporation System with Monitoring ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-200 Plate Handler and a plate adaptor

 

SPECIFICATIONS FOR THE ECM 830 GENERATOR

Display Type 7 inch color touchscreen
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
Fusing Type 3AG, 5 A, 250 V; Type T (time delay)
Environmental Characteristics 
Intended Use Indoor use only
Operating Temperature 10°C to 40°C
Cooling Convection through metal case
Relative Humidity 60%
Altitude 2,000 m (operating)

Mechanical Characteristics 
Footprint 12.75" x 11.25" x 8.5" (W x D x H)
Weight 16.0 lb (7.3 kg)

 

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface 7 inch Touchscreen User Interface
Input 110 to 240 VAC
Charge Time LV <7 s, HV <4 s
Pulse Length Range 10 μs – 999 μs LV Mode/ 1 μs resolution 1 msec – 999 msec LV Mode/ 1 msec resolution 1 sec – 10 sec LV Mode/ 0.1 sec resolution 10 μs – 600 μs HV Mode/ 1 μs resolution
Voltage Range 5 – 500 V LV Mode/ 1 V resolution 505 – 3,000 V HV Mode/ 5 V resolution
Multiple Pulsing 1 – 99
Pulse Interval 100 msec – 10 sec
Programmability Storage up to 1000 User Defined Protocols
Safety Pre-pulse Sample Resistance check, Over-current Pulse Abort, Instrument Arc Protection
Capacitance 3,775 μF LV, 85 μF HV
Remote Operation Remote PC operation and footswitch operation available

 

 

  • Mammalian cell transfection
  • In vitro, in vivo, ex vivo and in ovo tissue transfection
  • Nuclear transfer
  • Ex plant tissue transfection
  • Protoplast transfection
  • siRNA libraries
  • High throughput 25- and 96-well electroporation
  • CRISPR transfections
Item # Product
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, Coated, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 250 µl, 1 plate
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0211 Legacy ECM 830 Foot Pedal
45-0059 Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America)
45-0108 Flat Electrode
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0400 HT 100 Plate Handler, Manual Track Switching
45-0401 HT-200 Plate Handler, Auto-Sense Track Switching
45-0510 Needle L-Shaped Platinum Electrode Kit, 3 mm, with cables
45-0509 Needle L-Shaped Platinum Electrode, 3 mm
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0100 Petri Dish Electrode
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0130 Petri Pulser
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

ECM 830 Datasheet

 

ECM 830 User's Manual

 

ECM 830 Quick Start Guide

 

Legacy ECM 830 HT Technical Specifications

 

Legacy ECM 830 User's Manual

 

CRISPR Brochure

 

REFERENCES

  

Denies S, et al. In Vitro Exploration of a Myeloid-Derived Suppressor Cell Line as Vehicle for Cancer Gene Therapy. Cancer Gene Ther. 2017 Apr;24: 149-155.

 

Huang J, et al. Generation and Comparison of CRISPR-Cas9 and Cre-Mediated Genetically Engineered Mouse Models of Sarcoma. Nat Commun. 2017 Jul;8: 15999.

 

Sun Y, et al. Coinjection of IL2 DNA Enhances E7-Specific Antitumor Immunity Elicited by Intravaginal Therapeutic HPV DNA Vaccination with Electroporation. Gene Ther. 2017 Jul;24(7): 408-415.

 

Xia W, Jiao J. Histone Variant H3.3 Orchestrates Neural Stem Cell Differentiation in the Developing Brain. Cell Death Differ. 2017 Sep;24(9): 1548-1563.

 

Hsieh LS, et al. Convulsive Seizures from Experimental Focal Cortical Dysplasia Occur Independently of Cell Misplacement. Nature Commun. 2016 Jun;7: 11753.

 

Liu X, et al. Improved Anti-Leukemia Activities of Adoptively Transferred T cells Expressing Bispecific T-cell Engager in Mice. Blood Cancer J. 2016 Jun;6(6): e430.

 

Martell JD, et al. A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses. Nat Biotechnol. 2016 May;34: 774-780.

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Ploier B, et al. Dimerization Deficiency of Enigmatic Retinitis Pigmentosa-Linked Rhodopsin Mutants. Nature Commun. 2016 Oct;7: 12832.

 

Toda T, Shinmyo Y, Dinh Duong TA, Masuda K, Kawasaki H. An Essential Role of SVZ Progenitors in Cortical Folding in Gyrencephalic Mammals. Sci Rep. 2016;6: 29578.

 

Shinmyo Y, et al. CRISPR/Cas9-mediated Gene Knockout in the Mouse Brain using in Utero Electroporation. Sci Rep. 2016;6: 206111.

 

Varela JA, et al. Targeting Neurotransmitter Receptors with Nanoparticles In Vivo Allows Single-Molecule Tracking in Acute Brain Slices. Nature Commun. 2016;7: 10947.

 

Liu X, et al. T Cell Receptor-Induced NF-kB Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-Mediated Calcium EntryJ Biol Chem. 2016 Apr;291(16): 8440-8452.

 

Baumgart J, Grebe N. C57BL/6-specific conditions for efficient in utero electroporation of the central nervous systemJ Neurosci Methods. 2015 Jan;240: 116-124.

 

Kim NY, et al. Enhanced Immune Response to DNA Vaccine Encoding Bacillus Anthracis PA-D4 Protects Mice Against Anthrax Spore Challenge. PLoS One, 2015 Oct;10(10): e0139671.

 

Jiang JJ, et al. MicroRNA-26a Supports Mammalian Axon Regeneration In Vivo by Suppressing GSK3β Expression. Cell Death & Disease. 2015 Aug;6: e1865.

 

Rodriguez-Gil DJ, et al. Odorant Receptors Regulate the Final Glomerular Coalescence of Olfactory Sensory Neuron Axons. Proc Natl Acad Sci. 2015 May;112(18):5821-5826.

 

Simpson MT, et al. The Tumor Suppressor HHEX Inhibits Axon Growth When Prematurely Expressed in Developing Central Nervous System Neurons. Mol Cell Neurosci.  2015 Sep;68: 272-283.

 

Wang J, et al. Homology-Driven Genome Editing in Hematopoietic Stem and Progenitor Cells using ZFN mRNA and AAV6 Donors. Nature Biotechnol. 2015 Dec;33(12): 1256-1263.

 

Zhang H, et al. Time Point‐Based Integrative Analyses of Deep‐Transcriptome Identify Four Signal Pathways in Blastemal Regeneration of Zebrafish Lower Jaw. Stem Cells. 2015 Mar;33(3): 806-81.

 

Feng L, Kwon O, Lee B, Oh WC, Kim J. Using Mammalian GFP Reconstitution across Synaptic Partners (mGRASP) to Map Synaptic Connectivity in the Mouse Brain. Nat Protoc. 2014;9: 2425-2437.

 

Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo. Acta Biochim Biophys Sin; 2010; 42(2): 116-221.

 

Nasser, N. et al. Alternatively spliced Spalax heparanase inhibits extracellular matrix degradation, tumor growth, and metastasis. PNAS. 2009;106(7): 2253-2258.

 

Clape, C. et al. miR-143 Interferes with ERK5 Signaling, and Abrogates Cancer Progression in Mice. PLoS One. 2009 Oct;4(10): e7542.