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Pulse Switcher

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Pulse Switcher

Easily execute automatic grouped pulse, bipolar switching protocols by adding the BTX Pulse Switcher to your ECM 830 or Gemini X2 electroporation waveform generator.


Product Item # Price  
BTX Pulse Switcher 45-2061   View Price
Gemini X2 Pulse Switcher Bipolar Electroporation System 45-2062   View Price
ECM 830 Pulse Switcher Bipolar Electroporation System 45-0669   View Price
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Details

The BTX Pulse Switcher is a plug-in accessory and accompanying firmware package which upgrades the square wave pulsing capabilities of 7-inch touchscreen ECM 830 and Gemini X2 waveform generators. When both the BTX Pulse Switcher and accompanying firmware update are installed on the ECM 830 or Gemini X2, the system capabilities are enhanced to include the following features:

A new protocol category type, Pulse Switcher Protocols, is available.

Pulse switcher protocols allow a user to create and run protocols containing groups of square wave pulses with different polarity, pulse number, pulse length, and pulse interval characteristics.

  • Pulse Switcher User Defined Protocols may be programmed with up to 10 groups of square wave pulses with separately defined polarity (+ or -) for each group. The user may thus incorporate bipolar switching into their electroporation protocols.
  • The maximum number of pulses per group in a Pulse Switcher User Defined Protocol is 100 pulses.
  • The number of pulses, pulse length, and pulse interval is separately defined for each group of pulses in Pulse Switcher User Defined Protocols.
  • The minimum interval between pulses and between groups of pulses for Pulse Switcher User Defined Protocols is 50 ms.
  • The maximum number of pulses per Pulse Switcher User Defined Protocol is 1,000 pulses. 
  • 11 new Pulse Switcher Preset Electroporation Protocols have been added, utilizing bipolar switching capabilities for in vivo electroporation applications.

Square Wave User Defined Protocol features on the ECM 830 or Gemini X2 are enhanced with the installation of the Pulse Switcher.

  • The maximum number of pulses per Square Wave User Defined Protocol is increased to 100 pulses.
  • The minimum interval between pulses for Square Wave User Defined protocols is decreased to 50 ms.

Exponential Decay Wave pulsing features on the Gemini X2 are unaffected by the BTX Pulse Switcher.



Features & Benefits

  • Significantly expanded pulsing application capabilities—the ECM 830 and Gemini X2 solutions already have an established electroporation publication record, including use in CRISPR gene editing technologies. Adding a BTX Pulse Switcher to your system further expands your application protocol capabilities.
  • Ideal for high efficiency transfection of mammalian cells and tissues—including difficult to transfect cell types, such as stem cells and primary cells.
  • Simplify execution of advanced protocols—including automatic, hands-free bipolar switching.
  • Suitable for many applications—including in vitro, in vivo, in utero, in ovo, and ex vivo.


Included Items

Item # Product   Included Items
45-2061 Pulse Switcher   Pulse Switcher, Power Supply and USB Connector Cable
45-2062 Gemini X2 Pulse Switcher System   Gemini X2 Generator, Pulse Switcher, Power Supply and USB Connector Cable, Safety
Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack
45-0669

ECM 830 Pulse Switcher System

  ECM 830 Generator, Pulse Switcher, Power Supply and USB Connector Cable, Safety
Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack

Additional Accessories

The BTX Pulse Switcher is compatible with many accessories including:
  • BTX Safety Domes/Safety Stands for cuvette- and flatpack-based in vitro electroporation 
  • Specialty/in vivo electrodes with 4 mm diameter banana cables or 4 mm diameter banana cable adapters
  • HT-100 (Manual Column Switching) High-Throughput Electroporation Plate Handlers

 

  PULSE SWITCHER SQUARE WAVE PROTOCOL SPECIFICATIONS after Pulse Switcher Installation
 Voltage Range  Votage magnitude is constant for all groups
       LV Mode  5 to 500 V in 1 V steps
       HV Mode  505 to 3000 V in 5 V steps
 Pulse Groups  1 to 10
       For each group: 
       Voltage Polarity in Group  Positive or Negative
       Pulses in Group 
          LV Mode  1 to 100 pulses per group
          HV Mode  1 to 100 pulses per group
      Pulse Duration in Group 
          LV Mode  1 to 999 ms in 1 ms steps
 10 to 999 μs in 1 μs steps
          HV Mode  10 to 600 μs in 1 μs steps
       Pulse Interval in Group  0.05 to 10 s in 0.05 s steps
       Pulse-Group Interval   0.05 to 10 s in 0.05 s steps

 

 SQUARE WAVE PROTOCOL SPECIFICATIONS —after Pulse Switcher Installation
  Voltage Range (LV and HV Modes)  No change
  Pulse Duration (LV and HV Modes)  No change
  Multiple Pulsing (Square Wave) 
        LV Mode   1 to 100 pulses per sample
        HV Mode  1 to 1pp pulses per sample
 Pulse Interval  0.05 s to 10 s
  NOTE: Gemini X2 Exponential Decay Wave Protocol Specifications are unaffected by Pulse Switcher Intallation.

 

 PULSE SWITCHER SPECIFICATIONS 
 Compatible Generators  Gemini X2 and ECM 830*
 Power Supply Input Voltage Ratings  100 to 240 VAC, 50/60 Hz
 Pulse Switcher Input Voltate  5 VDC, 100 mA
 Warranty  2 years
 Storage Temperature  -10°C to 70°C (14°F to 158°F)
 Operating Temperature  5°C to 40°C (40°F to 104°F)
 Intended Use  Indoor Use Only
 Operating/Storage Humidity  20% to 80% RH, non-condensing
  Pollution Degree  IP2X
  Insulation Category  Category II
  Regulatory Certifications  CE, WEEE, RoHS
  Physical Chacteristics  
      Dimensions  8.9 x 7.8 x 5.4 cm (3.5 x 3.1 x 2.1 in)
      Weight  276 g (0.6 lb)
  * Compatible with 7-inch touchscreen instruments only.
  • In vitro suspension and adherent cell transfection
  • In vivo, in ovo and in utero gene and drug delivery
  • Explant tissue transfection
  • CRISPR gene editing
Item # Product
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0140 Cuvette Plus, 1 mm gap, 90 µl, Sterile, 24 Pkg/100 each, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0141 Cuvette Plus, 2 mm gap, 400 µl, Sterile, 24 Pkg/100 each, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0142 Cuvette Plus, 4 mm gap, 800 µl, Sterile, 24 Pkg/100 each, Yellow
47-0002 BTXpress Cytoporation Low Conductivity Medium T, 500 ml volume
47-0003 BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume
45-0801 BTXpress Solution, 2.0 ml Bottle for up to 20 Reactions
45-0802 BTXpress Solution, 5.0 ml Bottle for up to 50 Reactions
45-0805 BTXpress Solution, 10.0 ml Bottle for up to 100 Reactions
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0208 Cuvette Rack, 20 numbered positions
45-0400 HT 100 Plate Handler, Manual Track Switching
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0468 Replacement Pins for Plate Handler, Pkg. of 25
45-0469 Replacement Pins for Plate Handler, Pkg. of 100
5012-017 Pliers for HT Plate Handler
45-0466 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, coated, 1 plate
45-0466-M 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl,1 plate
45-0467 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, pkg. of 6
45-0462 25-Well Disposable Electroporation Plate, 4 mm gap, 200 µl, 1 plate
45-0463 25-Well Disposable Electroporation Plate, 4 mm gap, 200 µl, pkg. of 6
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, Coated, 1 plate
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 200 µl, 1 plate
45-00012 25-Well Plate Seal (1)
45-2030 Gemini X2, ECM 2001+, ECM 830, and ECM 630 Foot Pedal (compatible with touchscreen models)
45-0122 Genepaddles, 3 x 5 mm (need 45-0203 holder and 45-0216 cables)
45-0169 Genepaddles Kit, 3 x 5 mm (includes 45-0203 holder and 45-0216 cables)
45-0123 Genepaddles, 5 x 7 mm (need 45-0203 holder and 45-0216 cables)
45-0170 Genepaddles Kit, 5 x 7 mm (includes 45-0203 holder and 45-0216 cables)
45-0203 Genetrode/Genepaddle Holder
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0509 Needle L-Shaped Platinum Electrode, 3 mm
45-0510 Needle L-Shaped Platinum Electrode Kit, 3 mm, with cables
45-0530 Adherent Cell Electrode, 5 mm gap
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0130 Petri Pulser
45-0100 Petri Dish Electrode
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0492 Platinum Electrode Wand, 7 mm, positive
45-0490 Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 7 mm
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0502 Platinum Electrode Wand, 10 mm, positive
45-0500 Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 10 mm
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm
45-0121 2-Needle Array, 5 mm, Pkg. of 6
45-0206 2-Needle Array Handle, 5 mm, for 45-0168
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0120 2-Needle Array, 10 mm, Pkg. of 6
45-0205 2-Needle Array Handle, 10 mm, for 45-0167
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0101 Caliper Electrode , 1.0 cm X 1.0 cm, Brass, Transdermal Delivery Applications
45-0102 Caliper Electrode, 2.0 cm x 2.0 cm with extra 1.5 cm x 1.5 cm electrodes included, Stainless Steel, Electrochemotherapy Applications
45-0087 ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables
45-0216 ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)
45-0503 Mini Micro-Grabber Cables, Positive and Negative
45-0204 Adapter Banana Plug Cables, Red and Black (for use with Tweezertrodes and Adherent Cell Electrode)
45-0088 Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables
45-0217 ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)