800-272-2775
Details
The BTX Pulse Switcher is a plug-in accessory and accompanying firmware package which upgrades the square wave pulsing capabilities of 7-inch touchscreen ECM 830 and Gemini X2 waveform generators. When both the BTX Pulse Switcher and accompanying firmware update are installed on the ECM 830 or Gemini X2, the system capabilities are enhanced to include the following features:
A new protocol category type, Pulse Switcher Protocols, is available.
Pulse switcher protocols allow a user to create and run protocols containing groups of square wave pulses with different polarity, pulse number, pulse length, and pulse interval characteristics.
- Pulse Switcher User Defined Protocols may be programmed with up to 10 groups of square wave pulses with separately defined polarity (+ or -) for each group. The user may thus incorporate bipolar switching into their electroporation protocols.
- The maximum number of pulses per group in a Pulse Switcher User Defined Protocol is 100 pulses.
- The number of pulses, pulse length, and pulse interval is separately defined for each group of pulses in Pulse Switcher User Defined Protocols.
- The minimum interval between pulses and between groups of pulses for Pulse Switcher User Defined Protocols is 50 ms.
- The maximum number of pulses per Pulse Switcher User Defined Protocol is 1,000 pulses.
- 11 new Pulse Switcher Preset Electroporation Protocols have been added, utilizing bipolar switching capabilities for in vivo electroporation applications.
Square Wave User Defined Protocol features on the ECM 830 or Gemini X2 are enhanced with the installation of the Pulse Switcher.
- The maximum number of pulses per Square Wave User Defined Protocol is increased to 100 pulses.
- The minimum interval between pulses for Square Wave User Defined protocols is decreased to 50 ms.
Exponential Decay Wave pulsing features on the Gemini X2 are unaffected by the BTX Pulse Switcher.
Features & Benefits
- Significantly expanded pulsing application capabilities—the ECM 830 and Gemini X2 solutions already have an established electroporation publication record, including use in CRISPR gene editing technologies. Adding a BTX Pulse Switcher to your system further expands your application protocol capabilities.
- Ideal for high efficiency transfection of mammalian cells and tissues—including difficult to transfect cell types, such as stem cells and primary cells.
- Simplify execution of advanced protocols—including automatic, hands-free bipolar switching.
- Suitable for many applications—including in vitro, in vivo, in utero, in ovo, and ex vivo.
Included Items
| Item # | Product | Included Items | |
| 45-2061 | Pulse Switcher | Pulse Switcher, Power Supply and USB Connector Cable | |
| 45-2062 | Gemini X2 Pulse Switcher System | Gemini X2 Generator, Pulse Switcher, Power Supply and USB Connector Cable, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack |
|
| 45-0669 |
ECM 830 Pulse Switcher System |
ECM 830 Generator, Pulse Switcher, Power Supply and USB Connector Cable, Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack |
Additional Accessories
The BTX Pulse Switcher is compatible with many accessories including:- BTX Safety Domes/Safety Stands for cuvette- and flatpack-based in vitro electroporation
- Specialty/in vivo electrodes with 4 mm diameter banana cables or 4 mm diameter banana cable adapters
- HT-100 (Manual Column Switching) High-Throughput Electroporation Plate Handlers
| PULSE SWITCHER SQUARE WAVE PROTOCOL SPECIFICATIONS —after Pulse Switcher Installation | |
| Voltage Range | Votage magnitude is constant for all groups |
| LV Mode | 5 to 500 V in 1 V steps |
| HV Mode | 505 to 3000 V in 5 V steps |
| Pulse Groups | 1 to 10 |
| For each group: | |
| Voltage Polarity in Group | Positive or Negative |
| Pulses in Group | |
| LV Mode | 1 to 100 pulses per group |
| HV Mode | 1 to 100 pulses per group |
| Pulse Duration in Group | |
| LV Mode | 1 to 999 ms in 1 ms steps 10 to 999 μs in 1 μs steps |
| HV Mode | 10 to 600 μs in 1 μs steps |
| Pulse Interval in Group | 0.05 to 10 s in 0.05 s steps |
| Pulse-Group Interval | 0.05 to 10 s in 0.05 s steps |
| SQUARE WAVE PROTOCOL SPECIFICATIONS —after Pulse Switcher Installation | |
| Voltage Range (LV and HV Modes) | No change |
| Pulse Duration (LV and HV Modes) | No change |
| Multiple Pulsing (Square Wave) | |
| LV Mode | 1 to 100 pulses per sample |
| HV Mode | 1 to 1pp pulses per sample |
| Pulse Interval | 0.05 s to 10 s |
| NOTE: Gemini X2 Exponential Decay Wave Protocol Specifications are unaffected by Pulse Switcher Intallation. | |
| PULSE SWITCHER SPECIFICATIONS | |
| Compatible Generators | Gemini X2 and ECM 830* |
| Power Supply Input Voltage Ratings | 100 to 240 VAC, 50/60 Hz |
| Pulse Switcher Input Voltate | 5 VDC, 100 mA |
| Warranty | 2 years |
| Storage Temperature | -10°C to 70°C (14°F to 158°F) |
| Operating Temperature | 5°C to 40°C (40°F to 104°F) |
| Intended Use | For research use only |
| Operating/Storage Humidity | 20% to 80% RH, non-condensing |
| Pollution Degree | IP2X |
| Insulation Category | Category II |
| Regulatory Certifications | CE, WEEE, RoHS |
| Physical Chacteristics | |
| Dimensions | 8.9 x 7.8 x 5.4 cm (3.5 x 3.1 x 2.1 in) |
| Weight | 276 g (0.6 lb) |
| * Compatible with 7-inch touchscreen instruments only. | |
- In vitro suspension and adherent cell transfection
- In vivo, in ovo and in utero gene and drug delivery
- Explant tissue transfection
- CRISPR gene editing
| Item # | Product |
|---|---|
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0140 | Cuvette Plus, 1 mm gap, 90 µl, Sterile, 24 Pkg/100 each, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0141 | Cuvette Plus, 2 mm gap, 400 µl, Sterile, 24 Pkg/100 each, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0142 | Cuvette Plus, 4 mm gap, 800 µl, Sterile, 24 Pkg/100 each, Yellow |
| 47-0002 | BTXpress Cytoporation Low Conductivity Medium T, 500 ml volume |
| 47-0003 | BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume |
| 45-0801 | BTXpress Solution, 2.0 ml Bottle for up to 20 Reactions |
| 45-0802 | BTXpress Solution, 5.0 ml Bottle for up to 50 Reactions |
| 45-0805 | BTXpress Solution, 10.0 ml Bottle for up to 100 Reactions |
| 45-0110 | Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50 |
| 45-0109 | Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50 |
| 47-0206 | Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10 |
| 45-0207 | Safety Stand, Adjustable Gap (for use with Cuvettes) |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0400 | HT 100 Plate Handler, Manual Track Switching |
| 45-0465 | 25-Well Plate Adaptor for Plate Handlers |
| 45-0468 | Replacement Pins for Plate Handler, Pkg. of 25 |
| 45-0469 | Replacement Pins for Plate Handler, Pkg. of 100 |
| 5012-017 | Pliers for HT Plate Handler |
| 45-0466 | 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, coated, 1 plate |
| 45-0466-M | 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl,1 plate |
| 45-0467 | 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, pkg. of 6 |
| 45-0450 | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, Coated, 1 plate |
| 45-0450-M | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, 1 plate |
| 45-0452 | 96-Well Disposable Electroporation Plates, 4 mm gap, 200 µl, 1 plate |
| 45-00012 | 25-Well Plate Seal (1) |
| 45-2030 | Gemini X2, ECM 2001+, ECM 830, and ECM 630 Foot Pedal (compatible with touchscreen models) |
| 45-0122 | Genepaddles, 3 x 5 mm (need 45-0203 holder and 45-0216 cables) |
| 45-0169 | Genepaddles Kit, 3 x 5 mm (includes 45-0203 holder and 45-0216 cables) |
| 45-0123 | Genepaddles, 5 x 7 mm (need 45-0203 holder and 45-0216 cables) |
| 45-0170 | Genepaddles Kit, 5 x 7 mm (includes 45-0203 holder and 45-0216 cables) |
| 45-0203 | Genetrode/Genepaddle Holder |
| 45-0117 | Genetrodes, 1 mm L-Shape (GOLD TIP) |
| 45-0164 | Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0116 | Genetrodes, 3 mm L-Shape (GOLD TIP) |
| 45-0163 | Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0115 | Genetrodes, 5 mm L-Shape (GOLD TIP) |
| 45-0162 | Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0113 | Genetrodes, 5 mm Straight (GOLD TIP) |
| 45-0160 | Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables |
| 45-0114 | Genetrodes, 10 mm Straight (GOLD TIP) |
| 45-0161 | Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0509 | Needle L-Shaped Platinum Electrode, 3 mm |
| 45-0510 | Needle L-Shaped Platinum Electrode Kit, 3 mm, with cables |
| 45-0530 | Adherent Cell Electrode, 5 mm gap |
| 45-0531 | Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable |
| 45-0130 | Petri Pulser |
| 45-0100 | Petri Dish Electrode |
| 45-0504 | Petri Dish Platinum Electrode Chamber, 5 mm gap |
| 45-0505 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap |
| 45-0506 | Petri Dish Platinum Electrode Chamber, 15 mm gap |
| 45-0490 | Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 7 mm |
| 45-0502 | Platinum Electrode Wand, 10 mm, positive |
| 45-0500 | Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 10 mm |
| 45-0524 | Platinum Tweezertrode, 1 mm Flat |
| 45-0486 | Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables) |
| 45-0487 | Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable) |
| 45-0489 | Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables) |
| 45-0118 | Stainless Tweezertrode Electrode, 7 mm Diameter |
| 45-0165 | Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable) |
| 45-0488 | Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables) |
| 45-0119 | Stainless Tweezertrode Electrode, 10 mm Diameter |
| 45-0166 | Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable) |
| 45-0493 | Triple Electrode Tweezertrode, 3 mm |
| 45-0494 | Triple Electrode Tweezertrode, 5 mm |
| 45-0121 | 2-Needle Array, 5 mm, Pkg. of 6 |
| 45-0206 | 2-Needle Array Handle, 5 mm, for 45-0168 |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 45-0120 | 2-Needle Array, 10 mm, Pkg. of 6 |
| 45-0205 | 2-Needle Array Handle, 10 mm, for 45-0167 |
| 45-0167 | 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle |
| 45-0101 | Caliper Electrode , 1.0 cm X 1.0 cm, Brass, Transdermal Delivery Applications |
| 45-0102 | Caliper Electrode, 2.0 cm x 2.0 cm with extra 1.5 cm x 1.5 cm electrodes included, Stainless Steel, Electrochemotherapy Applications |
| 45-0087 | ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables |
| 45-0216 | ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish) |
| 45-0503 | Micrograbber Cable for Tissue Slice Chamber , Negative |
| 45-0204 | Adapter Banana Plug Cables, Red and Black (for use with Tweezertrodes and Adherent Cell Electrode) |
| 45-0088 | Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables |
| 45-0217 | ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode) |
