The Petri Pulser™ is a reusable electroporation applicator designed to fit into each single well of a 6-well plate or an individual 35 mm diameter petri dish. It consists of 13 gold plated electrodes spaced 2 mm apart. The Petri pulser consists of an electrode assembly embedded in a polyurethane holder with high voltage electrode cables. The thin electrodes are gold plated and designed to maximize the surface area of electroporation. The high voltage cables are connected with a BTX Pulse Generator, and the electrode head inserted into the dish or well containing the sample. An electroporation pulse may then be delivered. The entire applicator may be cleaned with mild detergent, and the electrodes may be sterilized with ethanol and dried with acetone.
The Petri Pulser is designed for the electroporation of adherent cells in situ or as an alternative to cuvette electroporation for larger volumes or multiple cell samples. The use of the electrode for the electroporation of adherent cells in situ eliminates the need for trypsinization or mechanical removal of cells from their growth substrate. This eliminates associated problems including low plating efficiencies following electroporation, interruption of cell cycle and interruption of intercellular communication.
MAMMALIAN CELL PROTEIN/DRUG ELECTROINCORPORATION
The Petri Pulser PP35-2P supplements our Petri Dish Electrode designed for 100 mm petri dishes, as the ideal electrode for in situ transfecting DNA into mammalian cells.1,2,3,4 This electrode has been used for electroincorporation of peptides and proteins in CHO cells5 and murine bone-marrow derived macrophages 1.
MAMMALIAN CELL TRANSFECTIONS/GENE THERAPY
The Petri Pulser PP35-2P is capable of supporting transfection protocols developed for our larger 100mm Petri Dish Electrode, including protocols for BHK and HUVEC.6 The electrode itself has been used for the transfection of 3T3-L1, K4 and W2 macrophages, Calu3, Rat adipose cells, SF295 human neuroglioblastoma and Cos 7 cells.7
PLANT, YEAST AND BACTERIAL ELECTROPORATION APPLICATIONS
Applications involving plant, yeast and bacterial electroporation may be carried out using the Petri Pulser. Scale up and multiple sample processing.
USE OF THE PETRI PULSER WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Petri Pulser.
|ECM 2001 System||45-0083 Coaxial Cables|| 45-0088 Female/Female Adapters
+ Petri Pulser
USE OF THE PETRI PULSER WITH THE GEMINI X2 ELECTROPORATION SYSTEM
In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Petri Pulser.
|Gemini X2 System||45-0217 Flat Electrode Cables|| 45-0088 Female/Female Adapters
+ Petri Pulser
|Voltage Range:||0 - 300 V|
|Pulse Length Range:||1 µsec - 35 msec|
|Volume Range:||0.5 - 3.0 ml|
|Number of Electrodes:||13|
|Electrode Thickness:||0.5 mm|
|Electrode Material:||Gold Plated|
|Generator Compatibility||ECM 399, ECM 630, ECM 830, ECM 2001 and Gemini X2|
|Pulse Length Range||1 µsec - 35 msec|
|Voltage Range||0 - 300 V|
|Volume Range||0.5 - 3.0 ml|
|Gap Size||2 mm|
- Mammalian Cell Transfections, Gene Therapy, Protein or Drug Delivery
- Plant/Yeast Applications