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Adherent Cell Electrodes

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Adherent Cell Electrodes
Reusable plate electrode designed to directly electroporate adherent cells or electroporate cultured cell monolayers on filters
Product Item # Price  
Adherent Cell Electrode, 5 mm gap 45-0530   View Price
Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable 45-0531   View Price
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Details

The Adherent Cell electrode is a reusable electroporation applicator designed to fit into each single well of a 12-well plate, 6-well plate or an individual 35 mm diameter Petri dish.

This plate electrode is specially designed to electroporate cultured monolayers of cells on laminin-coated filters, or on 12 mm polycarbonate or polyester transwell filter units (0.4 mm pore size). It can directly electroporate adherent cells such as human corneal keratocytes, Caco-2, dental pulp cells, NC65 and BCEC and MDCK cells.

The Adherent Cell Electrode consists of two parallel plate electrodes, 7 x 19 mm with 5 mm gap spacing between the plates. Each plate electrode has a 0.3 mm height insulated foot at both ends of the bottom. When the electrode is placed in a dish, a foot minimizes the damage on cells. The high voltage cables are connected to a BTX electroporator, and the electrode is brought down onto the filter until it makes contact with the buffer, usually at 1- 2 mm above the filter. A combination of HV and LV pulses may be used. Adherent cell electrodes are compatible with Gemini X2, ECM 830, ECM 2001+, and Legacy ECM 2001 Generators.

REQUIRED CABLES

Required for connection to ECM 830 and Gemini X2
45-0204 Adapter Banana Plug Cables, Red and Black
Required for connection to ECM 2001+
45-0204 Adapter Banana Plug Cables, Red and Black
45-0088 Female/Female Adapter Set for Banana Plug Cables
45-2057 or 45-2058 ECM 2001+ HV Output Cable, 5 ft or 10 ft length
Required for connection to Legacy ECM 2001
45-0204 Adapter Banana Plug Cables, Red and Black
45-0088 Female/Female Adapter Set for Banana Plug Cables
45-0083 Coaxial to Banana Plug Cables, Red and Black, 10 ft

 

USE OF ADHERENT CELL ELECTRODES WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS

In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode. 

+
 Gemini X2 System    45-0204 Female/Female Adapters 
+ Selected Adherent Cell Electrode

 

USE OF TWEEZERTRODES WITH ECM 2001+ ELECTROFUSION AND ELECTROPORATION SYSTEM

In this example, the ECM 2001+ generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode electrode.

+ + +
 ECM 2001+ System    45-2057 or 45-2058 HV Output Cable    45-0088 Female/Female Adapters    45-0204 Tweezertrode cable Adapters + Selected Adherent Cell Electrode

 

USE OF TWEEZERTRODES WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM

In this example, the Legacy ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode. 

+ + +
 Legacy ECM 2001 System    45-0083 Coaxial Cables    45-0083 Coaxial Cables    45-0204 Tweezertrode Cable Adapters + Selected Adherent Cell Electrode
Generator Compatibility ECM 830, ECM 2001+, Legacy ECM 2001, and Gemini X2
Pulse Length Range 1 µs to 35 ms
Voltage Range 0 to 300 V
Volume Range 0.5 to 3.0 ml
Autoclavable No
Field Type Homogeneous
Gap Size 5 mm
  • Mammalian Cell transfections
  • Direct electroporation onto adherent cells either growing on transwell filters or coverslips, or directly onto monolayers (such as polarized epithelial) without disruption
  • Plant and yeast ppplications
Adherent Cell Electrode User's Manual

 

Cleaning Electrodes

 

REFERENCES

 

1. Deora, A. et al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture, Traffic, 2007

 

2. BTX Protocols PR0835