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Details
The Flat Electrode can be used for both electroporation and electro cell fusion. The Flat Electrode generates either a divergent or homogeneous field depending on the orientation of the grooved electrodes. The Flat Electrode can be oriented with the grooved sides of the electrode facing one another to generate a divergent field for use in electro cell fusion. Alternatively, it can be oriented with the flat sides facing each other providing a homogeneous field for electroporation. The electrode is made of two rectangular, parallel plates of high grade stainless steel that are press-fitted into a polysulfone base.
REQUIRED CABLES
| Required for connection to ECM 830 and Gemini X2 | |
| 45-0217 | Banana to Banana Cables, Red and Black, 10 ft |
| Required for connection to ECM 2001+ | |
| 45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
| 45-0088 | Female/Female Adapters, Red and Black |
| 45-0217 | Banana to Banana Cables, Red and Black, 10 ft |
| Required for connection to Legacy ECM 2001 | |
| 45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
| 45-0088 | Female/Female Adapters, Red and Black |
| 45-0217 | Banana to Banana Cables, Red and Black, 10 ft |
USE OF THE FLAT ELECTRODE WITH GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS
In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the flat electrode.
 |
+ |  |
| Gemini X2 System | 45-0217 Banana to Banana Cables |
USE OF THE FLAT ELECTRODE WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM
In this example, the Legacy ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the flat electrode.
 |
+ |  |
+ |  |
+ | |
| ECM 2001+ System | 45-2057 or 45-2058 HV Output Cable | 45-0088 Female/Female Adapters | 45-0217 Banana to Banana Cables |
USE OF THE FLAT ELECTRODE WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the Legacy ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the flat electrode.
 |
+ |  |
+ | |
+ | |
| Legacy ECM 2001 System | 45-0083 Coaxial Cables | 45-0088 Female/Female Adapters | 45-0217 Banana to Banana Cables |
| Generator Compatibility | ECM 830, ECM 2001+, Gemini X2, and Legacy ECM 2001 |
| Field Type | Divergent or Homogeneous |
| Autoclavable | No |
- Cell fusion
- Hybridoma production
- Plant protoplast fusion
- Mammalian cell transfection
| Item # | Product |
|---|---|
| 45-0217 | ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode) |
REFERENCES
Long-Bo Cui et al. Transfer of germinal vesicle to ooplasm of young mice could not rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice. Human Reproduction, Vol. 20, No.6 pp. 1624-1631 (2005).
Grabarek et al. Efficient Delivery of dsRNA into Zona-Enclosed Mouse Oocytes and Preimplantation Embryos by Electroporation. Genesis 32:269-276 (2002)
