800-272-2775
Details
The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer.
ECM 830 GENERATOR
The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.
The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.
This instrument is intended for Research and Pre-Clinical use only and is not for use in diagnostic or clinical procedures.
FEATURES
- A wide range of voltages from 5 to 3,000 V
- Pulse durations from 10 μsec to 999 ms
- Pre-Pulse Resistance Check, Over-Current Pulse Abort, and Instrument Arc Protection Safety Features
- Easy to use touchscreen user interface
- Logs of every pulse delivered for QC and troubleshooting
IN VIVO APPLICATIONS
- Mammalian cell transfection
- In vitro, in vivo, ex vivo and in ovo tissue transfection
- Nuclear transfer
- Ex plant tissue transfection
- Plant protoplast transfection
- siRNA libraries
- High throughput 25- and 96-well electroporation
ADHERENT CELL TRANSFECTION
The ECM 830 generator coupled with the Petri Pulser electrode or the adherent cell electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser electrode is ideal for 6-well plates and the adherent cell electrodes for 12-well plates.
HIGH THROUGHPUT (HT) ELECTROPORATION (45-0664 and 45-0666)
The High Throughput (HT) 96- and 25-well systems offer the advantage of multi-well electroporation technology for processing multiple samples in seconds.
This permits a large number of samples to be quickly electroporated using high throughput plate handler technology. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.
The key to the HT System is the combination of the ECM 830 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 830 generator.
MONITORING OPTION
The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each electroporation experiment.
AUTOMATIC POLARITY SWITCHING
Combine the ECM 830 with the Pulse Switcher to incorporate automatic bipolar switching into electroporation protocols. Polarity switching enhances transfection efficiency by preventing transfectant accumulation at a single electrode and enhancing delivery to the target cells or tissue.
INCLUDED ITEMS
| Item # | Product | Included Items |
|---|---|---|
| 45-2052 | ECM 830 Electroporation System Complete, with Safety Dome | ECM 830 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 |
| 45-0661 | ECM 830 Electroporation System Complete, with Safety Stand | ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 |
| 45-0662 | ECM 830 Generator Only | ECM 830 Generator Only |
| 45-0665 | ECM 830 High Throughput Electroporation System, 96-well with HT-100 | ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a plate adaptor |
| 45-0666 | ECM 830 High Throughput Electroporation System, 96-well with HT-200 | ECM 830 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, 96-Well Plates (4 mm gap, 2X), Plate Seals, HT-200 Plate Handler and a plate adaptor |
| 45-0669 | ECM 830 Pulse Switcher Bipolar Electroporation System | ECM 830 Generator, Pulse Switcher, Power Supply and USB connector cable, Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm (pkg. of 30, 10 each), and Cuvette Rack |
SPECIFICATIONS FOR THE ECM 830 GENERATOR
| Display Type | 7 inch color touchscreen |
| Power Source Voltage | 100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle) |
| Fusing | Type 3AG, 5 A, 250 V; Type T (time delay) |
| Environmental Characteristics | |
| Intended Use | For research use only |
| Operating Temperature | 10°C to 40°C |
| Cooling | Convection through metal case |
| Relative Humidity | 60% |
| Altitude | 2,000 m (operating) |
| Mechanical Characteristics | |
| Footprint | 12.75" x 11.25" x 8.5" (W x D x H) |
| Weight | 16.0 lb (7.3 kg) |
STANDARD CAPABILITIES
| Operational Status | Internal self test upon start-up |
| Interface | 7 inch Touchscreen User Interface |
| Input | 110 to 240 VAC |
| Charge Time | LV <7 s, HV <4 s |
| Pulse Length | Range 10 μs – 999 μs LV Mode/ 1 μs resolution 1 msec – 999 msec LV Mode/ 1 msec resolution 1 sec – 10 sec LV Mode/ 0.1 sec resolution 10 μs – 600 μs HV Mode/ 1 μs resolution |
| Voltage Range | 5 – 500 V LV Mode/ 1 V resolution 505 – 3,000 V HV Mode/ 5 V resolution |
| Multiple Pulsing | 1 – 99 |
| Pulse Interval | 100 msec – 10 sec |
| Programmability | Storage up to 1000 User Defined Protocols |
| Safety | Pre-pulse Sample Resistance check, Over-current Pulse Abort, Instrument Arc Protection |
| Capacitance | 3,775 μF LV, 85 μF HV |
| Remote Operation | Remote PC operation and footswitch operation available |
- Mammalian cell transfection
- In vitro, in vivo, ex vivo and in ovo tissue transfection
- Nuclear transfer
- Ex plant tissue transfection
- Protoplast transfection
- siRNA libraries
- High throughput 25- and 96-well electroporation
- CRISPR transfections
| Item # | Product |
|---|---|
| 45-0109 | Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50 |
| 45-0110 | Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50 |
| 47-0206 | Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10 |
| 45-0167 | 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 45-0465 | 25-Well Plate Adaptor for Plate Handlers |
| 45-0450-M | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, 1 plate |
| 45-0450 | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, Coated, 1 plate |
| 45-0452 | 96-Well Disposable Electroporation Plates, 4 mm gap, 200 µl, 1 plate |
| 45-0531 | Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable |
| 45-0530 | Adherent Cell Electrode, 5 mm gap |
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0211 | Legacy ECM 830 Foot Pedal (compatible with non-touchscreen models) |
| 45-0108 | Flat Electrode |
| 45-0161 | Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0162 | Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0160 | Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables |
| 45-0117 | Genetrodes, 1 mm L-Shape (GOLD TIP) |
| 45-0164 | Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0114 | Genetrodes, 10 mm Straight (GOLD TIP) |
| 45-0116 | Genetrodes, 3 mm L-Shape (GOLD TIP) |
| 45-0163 | Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0115 | Genetrodes, 5 mm L-Shape (GOLD TIP) |
| 45-0113 | Genetrodes, 5 mm Straight (GOLD TIP) |
| 45-0106 | Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1 |
| 45-0105 | Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1 |
| 45-0103 | Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10 |
| 45-0104 | Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10 |
| 45-0400 | HT 100 Plate Handler, Manual Track Switching |
| 45-0401 | HT-200 Plate Handler, Auto-Sense Track Switching |
| 45-0510 | Needle L-Shaped Platinum Electrode Kit, 3 mm, with cables |
| 45-0509 | Needle L-Shaped Platinum Electrode, 3 mm |
| 45-0100 | Petri Dish Electrode |
| 45-0506 | Petri Dish Platinum Electrode Chamber, 15 mm gap |
| 45-0504 | Petri Dish Platinum Electrode Chamber, 5 mm gap |
| 45-0507 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap |
| 45-0505 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap |
| 45-0130 | Petri Pulser |
| 45-0486 | Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables) |
| 45-0524 | Platinum Tweezertrode, 1 mm Flat |
| 45-0487 | Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable) |
| 45-0489 | Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables) |
| 45-0488 | Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables) |
| 45-0207 | Safety Stand, Adjustable Gap (for use with Cuvettes) |
| 45-0119 | Stainless Tweezertrode Electrode, 10 mm Diameter |
| 45-0118 | Stainless Tweezertrode Electrode, 7 mm Diameter |
| 45-0166 | Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable) |
| 45-0165 | Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable) |
| 45-0493 | Triple Electrode Tweezertrode, 3 mm |
| 45-0494 | Triple Electrode Tweezertrode, 5 mm |
Pulse Switcher Quick Start Guide
Application Note: CRISPR Gene Editing of Developing Brain In Utero
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