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ECM 2001 Electrofusion & Electroporation Systems

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ECM 2001 Electrofusion & Electroporation Systems

MAMMALIAN CELL ENGINEERING YOUR WAY


Multi-purpose systems providing fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines. Meet your exact needs with preconfigured systems and a choice of  basic or advanced systems.


Please note: The LEGACY ECM 2001 Electro Cell Fusion & Electroporation System has been discontinued. The ECM 2001+ and ECM 2001 LITE are recommended alternatives.


Downloads and Firmware Updates


Product Item # Price  
ECM 2001+ Cell Fusion System, Complete 45-2045   View Price
ECM 2001+ Electroporation System Complete, for Cuvettes 45-2046   View Price
ECM 2001+ Embryo Manipulation System 45-2047   View Price
ECM 2001+ Hybridoma Production System 45-2048   View Price
ECM 2001+ Generator Only 45-2049   View Price
ECM 2001 LITE Cell Fusion System 45-2065   View Price
ECM 2001 LITE Electroporation System 45-2066   View Price
ECM 2001 LITE Embryo Manipulation System 45-2067   View Price
ECM 2001 LITE Hybridoma Production System 45-2068   View Price
ECM 2001 LITE Generator Only 45-2069   View Price
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Details

Get a multifunctional electrofusion and square wave electroporation generator that meets your exact needs. Available product configurations by application, such as embryo manipulation or hybridoma production, make choosing the right system easy.

For the ultimate in performance and convenience the ECM 2001+ offers advanced programming functions, preset protocols, and the most experimental flexibility.

For a reliable, affordable and easy-to-use cell fusion and mammalian transfection system, the ECM 2001 LITE may be just the right choice for you!


ECM 2001 SERIES GENERATORS

ECM 2001+ and ECM 2001 LITE are multifunctional electrofusion and square wave electroporation generators. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. These systems are powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

Waveforms
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.


ECM 2001+ ELECTROFUSION AND ELECTROPORATION SYSTEM

The ECM 2001+ Electrofusion and Electroporation System provides the highest performance and flexibility for cell fusion applications such as hybridoma production, hybrid cell formation, embryo manipulation, nuclear transfer and transgenic applications. Additionally, square wave electroporation functions offer an efficient, reproducible solution for transfecting mammalian cells and tissues with DNA, mRNA, siRNA, proteins, drugs, CRISPR gene editing constructs and nanoparticles.

Features & Benefits

  • Separate Electrofusion and Electroporation functional menus for streamlined protocol programming, data organization and navigation
  • Advanced User-Defined Electrofusion protocols enable users to independently combine up to 19 AC protocol steps and up 1 DC protocol step in any order, for the highest performance and flexibility
  • Convenient Pre-Set Electrofusion protocols for common hybridoma, embryo manipulation, and nuclear transfer applications
  • Electrofusion and Electroporation Quick Start menus for speedy access to most used 3-step electrofusion and most used 1-step electroporation parameters
  • Storage of up to 1,000 advanced User-Defined protocols

ECM 2001 LITE ELECTROFUSION AND ELECTROPORATION SYSTEM

The ECM 2001 LITE Electrofusion and Electroporation System offers a simple, effective option for cell fusion and square wave electroporation. The Quick Start Electrofusion menu offers a preconfigured 3-step protocol consisting of AC alignment, DC square wave, and AC. 

 Features & Benefits

  • Quick Start Electrofusion menu with 3-step AC-DC-AC protocol structure for speedy access to the most used experimental parameters
  • Storage of up to 1,000 User-Defined protocols with 3-step AC-DC-AC protocol structure
  • Flexibility to inactivate the Electrofusion protocol AC steps for use in square wave electroporation applications, or to inactivate the Electrofusion protocol DC step for embryo manipulation applications

APPLICATIONS

Electrofusion

Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC sine wave and DC square wave pulses. Fusion is achieved by the generation of an AC current sine waveform that generates a benign dielectrophoretic alignment of cells. Next, a DC square waveform is applied to promote fusion between aligned cells. After fusion, the AC sine waveform is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.

Mammalian Cell Transfection

Square wave electroporation is a highly flexible technique used to modify mammalian cells. Whether you are regulating gene expression up or down, expressing recombinant proteins, delivering non-cell-permeant molecules, or creating genetically modified model systems, electroporation is an ideal delivery method for all types of nucleic acids, proteins, drugs, and nanoparticles.

This method is non-toxic and requires no expensive reagents to successfully transfect your cells. Alternatively, adding additional selection steps to isolate stably transfected cells allows for integration of a gene into the genome of the cell for long term expression of protein.

The use of ECM 2001 series generators offers the control needed to adjust electrical settings for optimization of parameters. These systems are powerful enough to yield high transfection efficiencies and great survival rates for cell lines and difficult to transfect cell types including stem cells and primary cells.

In Vivo, In Utero & In Ovo Transfection

Square wave systems allow researchers to set the pulse lengths and number of pulses, which is critical to ensure viable cells and tissues while still maintaining efficient transfection both in vivo and ex vivo. Electroporation-mediated gene and drug delivery has been shown to substantially increase intracellular uptake and expression of DNA, siRNA and miRNA in muscle, skin, liver, kidney, testis, retina, tumors, etc. In vivo electroporation has successfully been used in embryo applications, in utero and in ovo applications, and in mammals, as well as other classical model systems such as chicken eggs, insects, and zebra fish.


PRODUCT CONFIGURATIONS BY APPLICATION

Complete Cell Fusion and Electroporation System

For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001 Cell Fusion Systems (45-2045, 45-2065) are our most versatile instrument configurations. The ECM 2001 Cell Fusion System comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.

Mammalian Cell Transfections

For mammalian cell electroporation, ECM 2001 Electroporation Systems (45-2046, 45-2066) come with a safety stand and cuvettes for small volume in vitro electroporation. ECM 2001 Electroporation Systems with Monitoring (45-2050, 45-2070) additionally include a high voltage probe, connection box and oscilloscope for precise wave form monitoring and quality control. Combine Electroporation Systems with electrodes and accessories available separately for large volume in vitro electroporation, in vivo electroporation, adherent electroporation, or cell fusion applications.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In these systems the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Hybridoma Production

For use in large volume cell fusion applications such as hybridoma production, ECM 2001 generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. These systems (45-2048, 45-2068) do not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Adherent Cell Transfections

Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001 series generators coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

Build Your Own Custom System

Combine ECM 2001 Generators (45-2049, 45-2069) with separately available BTX electrofusion and electroporation electrodes and accessories to tailor the system to your experimental needs.


INCLUDED ITEMS

Item #DescriptionIncluded Items
45-2045 ECM 2001+ Cell Fusion System  ECM 2001+ Generator, Microslides (0.5 mm gap, 3.2 mm gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack
45-2046 ECM 2001+ Electroporation System, for Cuvettes ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 
45-2047 ECM 2001+ Embryo Manipulation System ECM 2001 Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-2048 ECM 2001+ Hybridoma System  ECM 2001+ Generator, 2 ml Coaxial Optimization Chamber (470030), 9 ml Coaxial Production Chamber, Female/Female Adapter Set, Cytofusion Medium C (500 ml)
45-2049 ECM 2001+ Generator Only ECM 2001+ Generator, 5 ft High Voltage Output Cable, F/F adapter set
45-2065 ECM 2001 LITE Cell Fusion System ECM 2001 LITE Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack
45-0266 ECM 2001 LITE Electroporation System ECM 2001 LITE Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack
45-2067 ECM 2001 LITE Embryo Manipulation System ECM 2001 LITE Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber 
45-2068 ECM 2001 LITE Hybridoma Production System ECM 2001 LITE Generator, 2 ml Coaxial Optimization Chamber, 9 ml Coaxial Production Chamber, Female/Female Adapter Set, BTX Cytofusion Medium C 500 ml
45-2069 ECM 2001 LITE Generator Only ECM 2001 LITE Generator, 5 ft High Voltage Output Cable, F/F adapter setl

This instrument is intended for Research and Pre-Clinical use only and is not for use in diagnostic or clinical procedures.

 

 

  ECM 2001+ ECM 2001 LITE
Firmware Specifications 
Electrofusion Quick Start Menu  Up to 3 protocol steps, ordered AC-DC-AC  Up to 3 protocol steps, ordered AC-DC-AC
Electrofusion User Defined Protocols  Up to 19 AC protocol steps and up to 1 DC step, organized in any order  Up to 3 protocol steps, ordered AC-DC-AC
Electrofusion Preset Protocols  Over 20 Preset Protocols for electrofusion applications   Not avialable
Electroporation Quick State Menu   Yes   Not available
Electroporation User Defined Protocols   Yes   Not available
Square Wave Pulse, DC
Voltage Range 
   LV Mode  5 to 500 V in 1 V steps  5 to 500 V in 1 V steps
   HV Mode  505 to 3000 V in 1 V steps  505 to 3000 V in 1 V steps
 Voltage Accuracy 
   LV Mode  5%  5%
   HV Mode  5%  5%
 Pulse Length Range 
 LV Mode   10 to 999 µs in 1 µs steps  10 to 999 µs in 1 µs steps
 LV Mode  1 to 999 ms in 1 ms steps  1 to 999 ms in 1 ms steps
   HV Mode  10 to 600 µs in 1 µs steps  10 to 600 µs in 1 µs steps
 Multiple Pulsing 
   LV Mode  0 to 100 pulses per sample  0 to 100 pulses per sample
   HV Mode  0 to 100 pulses per sample  0 to 100 pulses per sample
 Pulse Interval  0.1 s to 10 s  0.1 s to 10 s
 Alignment (Pre-Pulse), AC 
 Frequency  0.2 to 2 MHz in 0.1 MHz steps  0.2 to 2 MHz in 0.1 MHz steps
 Voltage   5 to 75 V in 5 V steps   5 to 75 V in 5 V steps
 Duration   0 to 99 s in 1 s steps   0 to 99 s in 1 s steps
 Wave Shape   Sine wave   Sine wave
 Post-Fusion, AC 
 Frequency   0.2 to 2 MHz in 0.1 MHz steps   0.2 to 2 MHz in 0.1 MHz steps
 Voltage   5 to 75 V in 5 V steps   5 to 75 V in 5 V steps
 Duration   5 to 75 V in 5 V steps   5 to 75 V in 5 V steps
 Wave Shape   Sine wave   Sine wave
 Sample Load Ranges 
 AC    
   All Voltages   Load must be ≥60 Ω>  Load must be ≥60 Ω>
 DC    
   LV Mode  Pulse Length <100 ms,
 Load must be >8 to 9 Ω,
 Pulse Length >100 ms,
 Load must be >100 Ω
 Pulse Length <100 ms,
 Load must be >8 to 9 Ω,
 Pulse Length >100 ms,
 Load must be >100 Ω
   HV Mode  Pulse Length ≤600 ms
 Load must be ≥40 Ω
 Pulse Length ≤600 ms
 Load must be ≥40 Ω
 Other Specifications 
 Charging Time  LV = 7 s, HV = 7 s  LV = 7 s, HV = 7 s
 DC Monitoring  Protocol Name, Steps Executed,
 Time/Date, Error Conditions, Pulse
 Voltage, Pulse Current,
 Pulse Width, Pulse #, Droop %,
 Sample Voltage, Sample Current and
 Sample Resistance
 Protocol Name, Steps Executed,
 Time/Date, Error Conditions, Pulse
 Voltage, Pulse Current,
 Pulse Width, Pulse #, Droop %,
 Sample Voltage, Sample Current and
 Sample Resistance
 Display  7-inch full color display  7-inch full color display
 Controls  Touch Screen  Touch Screen
 Programmability  Stores over 1000 protocols  Stores over 1000 protocols
 DC Arc Control  Yes  Yes
 Pre-Pulse Sample Load Check  Ves (20 VDC)  Ves (20 VDC)
 Computer (Optional)  PC running Microsoft Windows XP, 7, 8.1 or 10  PC running Microsoft Windows XP, 7, 8.1 or 10
 Communications  USB  USB
 Data File Format  Comma Separated Values (CSV)  Comma Separated Values (CSV)
 Remote Operation  Yes, using Foot Pedal control  Yes, using Foot Pedal control
 Accessory Connector Type  Coaxial  Coaxial
 Power Ratings  70 W idle and 900 W pulsing  70 W idle and 900 W pulsing
 Input Voltage Ratings  100 to 240 VAC, 50/60 Hz  100 to 240 VAC, 50/60 Hz
 Dimensions (H x W x D)  13 x 12 x 13 in  13 x 12 x 13 in
 Weight  22 lb  22 lb
 Warranty  2 years  2 years
 Atmospheric Specifications 
   Operating Temperature   4°C to 40°C (40°F to 104°F)   4°C to 40°C (40°F to 104°F)
   Storage Temperature   -10°C to 70°C (14°F to 158°F)   -10°C to 70°C (14°F to 158°F)
   Operating Humidity  20% to 80% RH, non-condensing  20% to 80% RH, non-condensing
   Storage Humidity   20% to 80% RH, non-condensing   20% to 80% RH, non-condensing
 Mode of Operation  Continuous  Continuous
 Classification  Class I  Class I
 Pollution  IP2X  IP2X
 Installation  Category II  Category II
 Regulatory Certifications  CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme  CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme
  • Cell fusion
  • Hybridoma production
  • Nuclear transfer
  • Embryo manipulation
  • Mammalian cell transfection
  • Plant protoplast fusion
  • Stem cell production
Item # Product
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
47-0001 BTXpress Cytofusion Medium C, 500 ml volume
47-0003 BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume
47-0020 9 ml Coaxial Chamber for production
47-0030 Hybrimune 2 ml Coaxial Chamber for optimization
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0087 ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables
45-0217 ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)
45-0086 Legacy ECM 2001 CE Remote Control with 45-0085 cable
45-0088 Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables
45-0214 ECM 2001 Footswitch (for non-CE 2001 only)
45-0216 ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)
47-0208 ECM 2001 Safety Stand for Flatpack
45-0108 Flat Electrode
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0100 Petri Dish Electrode
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0130 Petri Pulser
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

ECM 2001+ Data Sheet

 

ECM 2001+ User's Manual

 

ECM 2001 LITE Data Sheet

 

ECM 2001 LITE User's Manual

 

Application Note: Efficient Electrofusion of Human B Cells and Humanized SP20 Myeloma for Hybridoma Production Applications

 

Cell Fusion Applications: Electrofusion vs. PEG

 

Declaration of Conformity

 

REFERENCES

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Sper RB, et al. Generation of a Stable Transgenic Swine Model Expressing a Porcine Histone 2B-eGFP Fusion Protein for Cell Tracking and Chromosome Dynamics Studies. PLoS One. 2017;12(1): e0169242.

 

Xie Z, et al. Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts. Sci Rep. 2017 Jun;7: 3036. doi: 10.1038/s41598-017-02785-y.

 

An L, et al. Efficient Generation of FVII Gene Knockout Mice using CRISPR/Cas9 Nuclease and Truncated Guided RNAs. Sci Rep. 2016;6: 25199. doi;10.1038/srep25199.

 

Bakhshi PK, Bain J, Gul MO, Stride E, Edirisinghe M, Staniland SS. Manufacturing Man‐Made Magnetosomes: High‐Throughput In Situ Synthesis of Biomimetic Magnetite Loaded Nanovesicles. Macromol Biosci. 2016;16: 1555-1561.

 

Huan, YJ, et al. Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Journal of Reproduction and Development. J Reprod Dec. 2016 Feb;62(1): 71-77. 

 

Jeong, YH, et al. Knock-in fibroblasts and transgenic blastocysts for expression of human FGF2 in the bovine β-casein gene locus using CRISPR/Cas9 nuclease-mediated homologous recombination. Zygote. 2016;24(3): 442-456.

 

Lu D, et al. Large-Scale Production of Functional Human Lysozyme from Marker-Free Transgenic Cloned Cows. Sci Rep. 2016;6: 22947. doi: 10.1038/srep22947.

 

Sun, Y, et al. Deletion of a Yci1 domain protein of Candida albicans allows homothallic mating in MTL heterozygous cells. mBio. 2016 Apr;7(2): e00465-16.

 

Natalie J, et al. H7N9 influenza virus neutralizing antibodies that possess few somatic mutationsJ Clin Invest. 2016 Apr;126(4): 1482-1494.

 

Wang K, et al. Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-Directed Repair. Mol TherNucleic Acids, 2016 Nov;5(11): e396.

 

Mangan, PR, et al. Dual inhibition of interleukin-23 and interleukin-17 offers superior efficacy in mouse models of autoimmunity. J Pharmacol Exp Ther. 2015 Aug;354(2), 152-165.

 

Ruiz N, et al. Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation. PLoS One. 2015 Jun; 10(6): e0130008.

 

Wang, K, et al. Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Sci Rep. 2015 Nov;13(5): 16623.

 

Wang Y, et al. MiR-499 Enhances the Cisplatin Sensitivity of Esophageal Carcinoma Cell Lines by Targeting DNA Polymerase β. Cell Physiol Biochem. 2015;36: 1587-1596.

 

Wu H, et al. TALE Nickase-Mediated SP110 Knockin Endows Cattle with Increased Resistance to Tuberculosis. Proc Natl Acad Sci USA. 2015 Mar;112(13): E1530-1539.

 

Zhang XH, Lian XD, Dai ZX, Zheng HY, Chen X, Zheng YT. α3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2015 Sep;199(6) :2030-2042.

 

Lai CW, et al. FTSJ2, a Heat Shock-Inducible Mitochondrial Protein, Suppresses Cell Invasion and Migration. PLoS One. 2014 Mar; 9(3): e90818.

 

Zhang Y, Luo W, Wang Y, Liu Y, and Zheng, L. Purified dendritic cell-tumor fusion hybrids supplemented with non-adherent dendritic cells fraction are superior activators of antitumor immunity. PLoS One, 2014;9(1), e86772.

 

Liu X, et al. Zinc-Finger Nickase-Mediated Insertion of the Lysostaphin Gene into the Beta-Casein Locus in Cloned Cows. Nat Comm. 2013;4: 2565.

 

Tan, C, et al. Impact of anti-CD25 monoclonal antibody on dendritic cell-tumor fusion vaccine efficacy in a murine melanoma model. J Transl Med. 2013 Jun;11: 148.

 

Wang Y, et al. Mir-655 Up-Regulation Suppresses Cell Invasion by Targeting Pituitary Tumor-Transforming Gene-1 in Esophageal Squamous Cell Carcinoma. J Transl Med. 2013;11: 301. 

 

Clow A, Gaynor P, Oback, B. A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusionBiomed Microdevices. 2010 Oct;12(5): 777-786.