The Petri Dish Electrode is designed to be used with a 100 mm petri dish that functions as the electroporation chamber. The Petri Dish Electrode is used to electroporate adherent cells or tissue grown in a petri dish.
To perform electroporation, simply add the exogenous molecule of interest into the electroporation buffer. The buffer can range in volume from 10 ml to 50 ml and is added to the cells grown in the plate. The electrode is lowered into the petri dish containing the sample and pulsed.
The electrode assembly has a 2 mm gap size. It contains parallel stainless steel electrodes which generate a homogeneous field. The Petri Dish Electrode is compatible with most BTX Generators.
USE OF THE PETRI DISH ELECTRODE WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Petri Dish Electrode.
|ECM 2001 System||45-0083 Coaxial Cables|| 45-0088 Female/Female Adapters
+ Petri Dish Electrode
|Generator Compatibility||ECM 630, ECM 830, ECM 2001 and Gemini X2|
|Voltage Range||0 – 2000 V|
|Volume Range||10 – 50 ml|
|Gap Size||2 mm|
|Pulse Length||10 µsec – 10 msec|
- Adherent mammalian cell transfections
- Plant tissue cell transfections