800-272-2775
Details
The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.
These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.
FEATURES & BENEFITS
- Square wave and exponential decay waves power in a single unit
- Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
- Large 7-inch, easy-to-use touch screen interface
- Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
- Preset protocols – includes the most common eukaryotic and prokaryotic cell types
- User-defined protocols – unlimited ability to add and modify protocols
- Safety – displays resistance measurements for each pulse with three layers of arc protection
- Data management – stores logs of every pulse delivered for QC and troubleshooting
GEMINI SC SYSTEM
The Gemini SC system is designed specifically for cuvette-based in vitro transfections of eukaryotic or prokaryotic cells in suspension. With a wide range of pulsing parameters, advanced safety features as well as dozens of preset protocols, the Gemini SC is ideal for any lab requiring efficient cell transfection or transformation without the use of costly reagents.
GEMINI X2 AND GEMINI X2 HT and GEMINI X2 PULSE SWITCHER SYSTEMS
The Gemini X2 system provides the ultimate experimental flexibility. This one easy setup allows for transfections in cuvettes or high throughput plates, or the capability to utilize the wide variety of BTX specialty electrodes for specific applications. In addition, Gemini X2 electrical output specifications are wide-ranging, making it the most versatile electroporation system available today.
Electroporation of suspension cells can be achieved in cuvettes (X2) or 96-well plates (X2 HT) with an HT plate handler. Additionally, the Gemini X2 can be paired with BTX specialty electrodes to deliver gene and drugs in vivo, in utero, in ovo and to explant tissues as well as adherent cells.
Combine the Gemini X2 with the Pulse Switcher to incorporate automatic bipolar switching into electroporation protocols. Polarity switching enhances transfection efficiency by preventing transfectant accumulation at a single electrode and enhancing delivery to the target cells or tissue.
GEMINI'S BROAD CAPABILITIES
Exponential Decay/Square Twin Waveforms
Allows researchers to select which type of waveform they would like to use to transfect eukaryotic cells or transform prokaryotic cells. Typically, eukaryotic cells respond best to square wave pulses while prokaryotic cell respond well to exponential decay wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their application.
Simple User Interface
Permits intuitive touch screen or computer-controlled protocol programing and execution as well as password protection of protocols.Each system is programmable and offers unlimited protocol storage as well as dozens of proven pre-set protocols to reduce optimization time. The X2 systems provide protocol delivery log storage. Logs can be accessed for quality control, optimization or troubleshooting purposes.
Pre-Pulse Resistance Measurement
Offers assurance that sample resistance is within acceptable range for electroporation. This feature protects precious sample as well as system component to safeguard against arcing.
Widest Voltage/Pulse length Range Available
Provides researchers with the widest range of voltage options available in the market.
- The Gemini X2 can achieve voltage delivery as low as 5 V and as high as 3000 V in both waveforms in addition to pulse lengths of 10 µs to 5 s, from delicate in ovo tissue to hearty gram positive bacteria.
- The Gemini SC can achieve voltage delivery as low as 10 V and as high as 3000 V in both waveforms in addition to pulse lengths of 50 µs to 5 s.
Highly Accurate Pulsing
Delivers precise voltage, pulse length/time constant, pulse interval and displays the values back to the researcher allowing for visual confirmation of successful electroporation.
Revolutionary Arc Proofing Safety Features
Gives researchers peace of mind knowing that their sample and their systems are safe from troublesome arcing with enhanced 4-way safety checks through the pulsing procedure. Using two separate pre pulse, sample resistance monitoring scans to ensure proper conditions, as well as internal safety features auditing pulse progress in at the component as well as software level, arcing is dramatically decreased.
This instrument is intended for Research and Pre-Clinical use only and is not for use in diagnostic or clinical procedures.
INCLUDED ITEMS
| Item # | Description | Included Items |
|---|---|---|
| 45-2040 | Gemini X2 Electroporation System Complete for Cuvettes | Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack 660 |
| 45-2041 | Gemini X2 Electroporation Generator Only | Gemini X2 Electroporation Generator only |
| 45-2042 | Gemini SC Electroporation System Complete for Cuvettes | Gemini SC Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. 30 (10 each), and Cuvette Rack 660 |
| 45-2043 | Gemini SC Electroporation Generator Only | Gemini SC Electroporation Generator only |
| 45-2044 | Gemini X2 High Throughput System, 96-well with HT-200 | Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and cuvette Rack 660, HT-200 Plate Handler, 2 mm gap HT plate and 4 mm gap HT plate |
| 45-2062 | Gemini X2 Pulse Switcher Bipolar Electroporation System |
Gemini X2 Generator, Pulse Switcher, Power Supply and USB connector cable, Safety Dome, Cuvettes 1 mm, 2 mm,4 mm (pkg. of 30, 10 each), and Cuvette Rack |
| SQUARE WAVE PULSE RANGES | |
| Voltage Range | |
| LV Mode | 5 to 500 V in 1 V steps |
| HV Mode | 505 to 3000 V in 5 V steps |
| Voltage Accuracy | |
| LV Mode | 5% |
| HV Mode | 5% |
| Pulse Length Range | |
| LV Mode | 10 μs to 999 μs in 1 μs steps 1 ms to 999 ms in 1 ms steps |
| HV Mode | 10 μs to 600 μs in 1 μs steps |
| Multiple Pulsing | |
| Single Samples | 1 to 99 pulses per sample |
| HT Plate Handler Mode | 1 to 10 pulses per sample (up to 120 total) |
| Pulse Interval | 0.1 s to 10 s |
| Capacitance | |
| LV Mode | 3775 μF |
| HV Mode | 85 μF |
| Voltage Droop | 20% Load <1 K Ω, 10% Load >1 K Ω |
| EXPONENTIAL DECAY PULSE RANGES | |
| Voltage Range | |
| LV Mode | 5 to 500 in 1 V steps |
| HV Mode | 505 to 3000 in 5 V steps |
| Voltage Accuracy | |
| LV Mode | 5% |
| HV Mode | 5% |
| Time Constant Range | |
| LV Mode | 1 ms to 5.158 s |
| HV Mode | 0.5 ms to 133.875 ms |
| Multiple Pulsing | |
| Multiple Pulsing | 1 to 2 depending on device's current amplitude limit |
| Pulse Interval | 5 to 30 s |
| Capacitance | |
| LV Mode | 25 to 3275 μF in 25 μF steps |
| HV Mode | 10, 25, 35, 50, 60, 75, 85 μF |
| Available Internal Resistance Selections | |
| All Modes | LV 25 to 1575 Ω in 25 Ω steps HV 50 to1575 Ω in 25 Ω steps |
| SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE | |
| LV Mode | PL ≤100 ms, load ≥8 to 9 Ω, PL >100 ms, load ≥100 Ω |
| HV Mode | Load ≥40 Ω |
| SQUARE WAVE PULSE RANGES | |
| Voltage Range | |
| LV Mode | 10 to 500 V in 5 V steps |
| HV Mode | 510 to 3000 V in 10 V steps |
| Voltage Accuracy | |
| LV Mode | 5% |
| HV Mode | 5% |
| Pulse Length Range | |
| LV Mode | 0.05 ms to 10 ms in 0.05 ms steps 10 ms to 100 ms in 1 ms steps |
| HV Mode | 50 μs to 5 ms in 50 μs steps |
| Multiple Pulsing | |
| LV Mode | 1 to 10 pulses per sample |
| HV Mode | 1 to 2 pulses per sample |
| Pulse Interval | 0.1 s to 10 s |
| Capacitance | |
| LV Mode | 3775 μF |
| HV Mode | 85 μF |
| Voltage Droop | 20% Load <1 K Ω, 10% Load >1 K Ω |
| EXPONENTIAL DECAY PULSE RANGES | |
| Voltage Range | |
| LV Mode | 10 to 500 V in 5 V steps |
| HV Mode | 510 to 3000 V in 10 V steps |
| Voltage Accuracy | |
| LV Mode | 5% |
| HV Mode | 5% |
| Time Constant Range | |
| LV Mode | 1.25 ms to 3.275 s / 1.25 ms |
| HV Mode | 0.5 ms to 50 ms / 0.5 ms |
| Multiple Pulsing | |
| Multiple Pulsing | No |
| Pulse Interval | N/A |
| Capacitance | |
| LV Mode | 25 to 3275 μF in 25 μF steps |
| HV Mode | 10, 25 and 50 μF |
| Available Internal Resistance Selections | |
| All Modes | 50 to 1000 Ω in 50 Ω steps |
| SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE | |
| LV Mode | Load ≥10 Ω |
| HV Mode | 510 - 2500 V Load ≥20 Ω, 2500 - 3000 V, Load ≥600 Ω |
OTHER SPECIFICATIONS (X2 AND SC)
| Charging Time | LV <7 s, HV <4 s |
| Monitoring | Pulse Voltage, Width, Droop % and Sample Resistance |
| Display | 7.0-in color display |
| Controls | Touch Screen |
| Programmability | Storage over 1,000 Protocols |
| Arc Control | Yes |
| Interlock Switches | Yes |
| Pre-Pulse Sample Resistance Check | Yes |
| Pulse Over Current Protection | Yes |
| PC Control | X2: Yes; SC: No |
| PC Communications | X2: Yes; SC: No |
| Log Report | X2: Yes; SC: No |
| Remote Operation | X2: Yes; SC: No |
| Foot Switch Control | X2: Yes; SC: No |
| Power Ratings | 35 W idle and 350 W pulsing |
| Input Voltage Ratings | 100 to 240 VAC |
| CE, ETL Marking, CB Scheme, EuRoHS | Yes |
| Dimensions (H x W x D) | 12.75 x 11.25 x 8.5 |
| Weight | ~16 lb |
| Warranty | 2 years |
| Atmospheric Specifications | |
| Operating Temperature | 4˚C to 40˚C (40˚F to 104˚F) |
| Storage Temperature | -10˚C to 70˚C (14˚F to 158˚F) |
| Storage Humidity | 20% to 80% RH, non condensing |
| Mode of Operation | Continuous |
| Classification | Class I |
| Pollution | Degree I |
| Installation | Category II |
| Regulatory Certifications | CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme |
- CRISPR transfections
- Suspension and adherent cells
- In vivo, in ovo and in utero gene and drug delivery
- Tissue explants
- Transformation of eukaryotic and prokaryotic cells
- High throughput electroporation
| Item # | Product |
|---|---|
| 45-0110 | Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50 |
| 47-0206 | Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10 |
| 45-0109 | Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50 |
| 45-0167 | 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 45-0465 | 25-Well Plate Adaptor for Plate Handlers |
| 45-0466 | 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, coated, 1 plate |
| 45-0467 | 25-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, pkg. of 6 |
| 45-0450-M | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, 1 plate |
| 45-0450 | 96-Well Disposable Electroporation Plate, 2 mm gap, 100 µl, Coated, 1 plate |
| 45-0452 | 96-Well Disposable Electroporation Plates, 4 mm gap, 200 µl, 1 plate |
| 45-0531 | Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable |
| 45-0530 | Adherent Cell Electrode, 5 mm gap |
| 45-0101 | Caliper Electrode , 1.0 cm X 1.0 cm, Brass, Transdermal Delivery Applications |
| 45-0102 | Caliper Electrode, 2.0 cm x 2.0 cm with extra 1.5 cm x 1.5 cm electrodes included, Stainless Steel, Electrochemotherapy Applications |
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0108 | Flat Electrode |
| 45-2030 | Gemini X2, ECM 2001+, ECM 830, and ECM 630 Foot Pedal (compatible with touchscreen models) |
| 45-0169 | Genepaddles Kit, 3 x 5 mm (includes 45-0203 holder and 45-0216 cables) |
| 45-0170 | Genepaddles Kit, 5 x 7 mm (includes 45-0203 holder and 45-0216 cables) |
| 45-0122 | Genepaddles, 3 x 5 mm (need 45-0203 holder and 45-0216 cables) |
| 45-0123 | Genepaddles, 5 x 7 mm (need 45-0203 holder and 45-0216 cables) |
| 45-0161 | Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0162 | Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0160 | Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables |
| 45-0117 | Genetrodes, 1 mm L-Shape (GOLD TIP) |
| 45-0164 | Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0114 | Genetrodes, 10 mm Straight (GOLD TIP) |
| 45-0116 | Genetrodes, 3 mm L-Shape (GOLD TIP) |
| 45-0163 | Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0115 | Genetrodes, 5 mm L-Shape (GOLD TIP) |
| 45-0113 | Genetrodes, 5 mm Straight (GOLD TIP) |
| 45-0106 | Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1 |
| 45-0105 | Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1 |
| 45-0103 | Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10 |
| 45-0104 | Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10 |
| 45-0400 | HT 100 Plate Handler, Manual Track Switching |
| 45-0401 | HT-200 Plate Handler, Auto-Sense Track Switching |
| 45-0100 | Petri Dish Electrode |
| 45-0506 | Petri Dish Platinum Electrode Chamber, 15 mm gap |
| 45-0504 | Petri Dish Platinum Electrode Chamber, 5 mm gap |
| 45-0507 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap |
| 45-0505 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap |
| 45-0130 | Petri Pulser |
| 45-0486 | Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables) |
| 45-0524 | Platinum Tweezertrode, 1 mm Flat |
| 45-0487 | Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable) |
| 45-0489 | Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables) |
| 45-0488 | Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables) |
| 45-2020 | Safety Dome for the Gemini SC |
| 45-2021 | Safety Dome for the Gemini X2 |
| 45-0207 | Safety Stand, Adjustable Gap (for use with Cuvettes) |
| 45-0119 | Stainless Tweezertrode Electrode, 10 mm Diameter |
| 45-0118 | Stainless Tweezertrode Electrode, 7 mm Diameter |
| 45-0166 | Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable) |
| 45-0165 | Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable) |
| 45-0493 | Triple Electrode Tweezertrode, 3 mm |
| 45-0494 | Triple Electrode Tweezertrode, 5 mm |
Pulse Switcher Quick Start Guide
Application Note: CRISPR Gene Editing of Developing Brain In Utero
Documentation (ZIP 11.9MB)
REFERENCES
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Seyed Jafari SM, et al. Efficacy of In Vivo Electroporation-Mediated IL-10 Gene Delivery on Survival of Skin Flaps. J Membr Biol. 2017 Aug. doi: 10.1007/s00232-017-9974-x. [Epub ahead of print]
Sun, L. et al. Glutamine is required for snakehead fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle. J Gen Virol, 2016 Nov;97(11): 2489-2855.
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