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ECM 630 Exponential Decay Wave Electroporation System

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ECM 630 Exponential Decay Wave Electroporation System

Electroporation system primarily used for bacteria and yeast transformation. High throughput (25- and 96-well) models available.


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Product Item # Price  
Legacy ECM 630 Electroporation System Complete, for Cuvettes 45-0001   View Price
ECM 630 Generator Only 45-0652   View Price
ECM 630 Electroporation System Complete, for Cuvettes with Monitoring 45-0655   View Price
ECM 630 Electroporation System Complete, with Safety Stand 45-0651   View Price
ECM 630 High Throughput Electroporation System, 96-well with HT-100 45-0654   View Price
ECM 630 Electroporation System Complete, with Safety Dome 45-2051   View Price
ECM 630 High Throughput Electroporation System, 25-well with HT-100 45-0653   View Price
ECM 630 High Throughput Electroporation System with Monitoring 45-0656   View Price
Legacy ECM 630 Generator Only 45-0051   View Price
Legacy ECM 630 High Throughput Electroporation System, 25-well with HT-100 45-0412   View Price
Legacy ECM 630 High Throughput Electroporation System, 96-well with HT-100 45-0422   View Price
Legacy ECM 630 Electroporation System Complete, for Cuvettes with Monitoring 45-0071   View Price
Legacy ECM 630 High Throughput Electroporation System with Monitoring 45-0471   View Price
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Details

ECM 630 GENERATOR

The ECM 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. The ability to select the resistance and capacitance values, and adjust the range of voltages is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection.  The ECM 630 can perform the widest range of electroporation applications among commercially available electroporation generators. Legacy ECM 630 systems are available while supplies last as we transition to new ECM 630 instruments.

Flexibility is important, so BTX has designed a plug and play system for our ECM 630 system to transition between standard cuvettes and to 25/96-well plates using High Throughput Plate Handlers.

The ECM 630 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

NEW ECM 630 FEATURES

  • Large, easy to use color touchscreen interface
  • Preset protocols for transforming the most common bacteria, yeast, and microorganisms
  • High-Throughput Plate Handler setting for automatic processing of up to 96 samples at a time with an HT-200 Plate Handler
  • Create, modify, and store up to 1,000 user defined protocols
  • Enhanced Safety Features: Pre-pulse sample resistance check, arc protection, and over-current pulse abort
  • Data Management: Experimental data log stores parameters of all pulses delivered and every sample resistance for QC and troubleshooting purposes
  • Voltage range of 5 V to 3,000
  • Capacitance range of 25 µF to 3,275 µF in 25 µF steps in LV mode; 10, 25, 35, 50, 60, 75, 85 µF in HV mode.
  • Resistance range of 25 ohm to 1,575 ohm in 25 ohm steps.

LEGACY ECM 630 FEATURES

  • Digital user interface; push button rotary knob to set parameters
  • Voltage range of 10 V to 2,500 V
  • Capacitance Range of 25 µF to 3,275 µF in 25 µF steps
  • Resistance range of 25 ohm to 1,525 ohm in 25 ohm steps. The additional resistor selection "None" allows researchers to reproduce protocols from competitive systems

APPLICATIONS

  • Transformation of bacteria and yeast
  • Transfection of mammalian cells
  • Transfection of plant tissue and plant protoplasts
  • High throughput 25- and 96-well electroporation

HIGH THROUGHPUT (HT) ELECTROPORATION

The High Throughput (HT) 96- and 25-well systems offer multi-well electroporation technology for processing multiple samples in seconds. Using the HT multi-well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.

The key to the HT System is the combination of the ECM 630 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 630 generator. The HT-100 Plate Handler provides manual track switching (45-0412, 45- 0422). New ECM 630 instruments are also compatible with HT-200 plate handlers for automatic processing of up to 96 samples at a time.

MONITORING OPTION

Addition of the ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment.

INCLUDED ITEMS

Item #ProductIncluded Items
45-0001 Legacy ECM 630 Electroporation System Complete, for Cuvettes Legacy ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0652 ECM 630 Generator Only ECM 630 Generator Only
45-0655 ECM 630 Electroporation System Complete, for Cuvettes with Monitoring ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0651 ECM 630 Electroporation System Complete, with Safety Stand ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), and Cuvette Rack 660
45-0654 ECM 630 High Throughput Electroporation System, 96-well with HT-100 ECM 630 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-2051 ECM 630 Electroporation System Complete, with Safety Dome ECM 630 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), and Cuvette Rack 660
45-0653 ECM 630 High Throughput Electroporation System, 25-well with HT-100 ECM 630 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0656 ECM 630 High Throughput Electroporation System with Monitoring ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-100 Plate Handler and a Plate Adaptor
45-0051 Legacy ECM 630 Generator Only Legacy ECM 630 Generator Only
45-0412 Legacy ECM 630 High Throughput Electroporation System, 25-well with HT-100 Legacy ECM 630 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0422 Legacy ECM 630 High Throughput Electroporation System, 96-well with HT-100 Legacy ECM 630 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0071 Legacy ECM 630 Electroporation System Complete, for Cuvettes with Monitoring Legacy ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0471 Legacy ECM 630 High Throughput Electroporation System with Monitoring Legacy ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-100 Plate Handler and a Plate Adaptor

 

 SPECIFICATIONS FOR THE ECM 630 GENERATOR

Display Type 7 inch color touchscreen interface
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT II Power 350 W (Pulsing), 35 W (Idle)
Fusing Type 3AG, 5 A, 250 V 1/4 x 1 1/4 in; Type T (time delay)
Environmental Characteristics
Intended Use Indoor use only
Operating Temperature 4° C to 40° C
Cooling Convection through metal case
Relative Humidity 20% to 80% RH, non-condensing
Mechanical Characteristics 
Footprint 12.75" x 11.25" x 8.5" (W x D x H)
Weight 16.0 lb (7.3 kg)
Controls Touchscreen, On/Off power switch

     

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface 7 inch color touchscreen user interface
Programmability Storage over 1000 Protocols
Data Management Experimental Log of Pulse Parameters and Sample Resistance
Input 100 V to 240 VAC Universal
Charge Time LV < 7 s, HV < 4 s
Arc Control Yes
Voltage Range LV Mode 5 - 500 V in 1 V steps; HV Mode 505 - 3000 V in 5 V steps
Capacitance Range LV Mode 25 µF to 3,275 µF in 25 µF steps; HV Mode 10, 25, 35, 50, 60, 75, 85 µF
Resistance Range LV Mode 25 ohm - 1,575 ohm in 25 ohm steps;  HV Mode 50 ohm - 1,575 ohm in 25 ohm steps

 

 

 SPECIFICATIONS FOR THE LEGACY ECM 630 GENERATOR

Display Type 20-character by 4-line liquid crystal display. LED backlit 
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
Fusing
2.5 A, T rating 250 V 
Environmental Characteristics
Intended Use Indoor use only
Operating Temperature 10° C to + 40° C
Cooling Convection through metal case
Relative Humidity 60%
Altitude 2,000 m (operating)
Mechanical Characteristics 
Footprint 12.5" x 12.25" x 5.5" (W x D x H)
Weight 10.0 lb (4.5 kg)
Controls Single rotary knob with push button toggle to set parameters, On/Off power and Start switches

     

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V / 220 V Universal
Charge Time 5 sec maximum
Arc Control Arc Quenching™
Voltage Range 10 - 500 V LV Mode / 1 V resolution 50 - 2500 V HV Mode/ 5 V resolution
Capacitance Range 1 µF, 25 µF to 3,275 µF LV Mode 25 µF, 50 µF HV Mode
Resistance Range 25 ohm - 1,575 ohm/ 25 ohm resolution HV & LV Modes; "None" setting to simulate alternative systems lacking resistance control LV Mode

  • Transformation of bacteria and yeast
  • Transfection of mammalian cells
  • Transfection of plant cells & plant protoplasts
  • High throughput 25/96-well
Item # Product
45-0466 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, coated, 1 plate
45-0467 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, pkg. of 6
45-0462 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, 1 plate
45-0463 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, pkg. of 6
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, Coated, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 250 µl, 1 plate
45-0530 Adherent Cell Electrode, 5 mm gap
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0400 HT 100 Plate Handler, Manual Track Switching
45-0401 HT-200 Plate Handler, Auto-Sense Track Switching
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)

ECM 630 Datasheet

 

ECM 630 User's Manual

 

ECM 630 Quick Start Guide

 

Legacy ECM 630 Technical Specifications

 

Legacy ECM 630 User's Manual

 

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