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AgilePulse Waveform Electroporation System


AgilePulse Waveform Electroporation System
Advanced electroporation solution providing maximum efficiency DNA delivery for vaccine development. Large volume option for fast, efficient transfection of up to 10 mL cell suspension.


Product Item # Price  
AgilePulse In Vivo ID (Intra-Dermal) System (N. America) 47-0400N   View Price
AgilePulse In Vivo ID (Intra-Dermal) System 47-0400NINT   View Price
AgilePulse In Vivo ID (Intra-Dermal) Generator Only (N. America) 47-0401N   View Price
AgilePulse In Vivo ID (Intra-Dermal) Generator Only 47-0401NINT   View Price
AgilePulse In Vivo IM (Intra-Muscular) System (N. America) 47-0500N   View Price
AgilePulse In Vivo IM (Intra-Muscular) System 47-0500NINT   View Price
AgilePulse In Vivo IM (Intra-Muscular) Generator (N. America) 47-0501N   View Price
AgilePulse In Vivo IM (Intra-Muscular) Generator 47-0501NINT   View Price
AgilePulse MAX Large Volume Transfection System, Complete (N. America) 47-0200N   View Price
AgilePulse MAX Large Volume Transfection System, Complete 47-0200NINT   View Price
AgilePulse MAX Waveform Generator (N. America) 47-0201N   View Price
AgilePulse MAX Waveform Generator 47-0201NINT   View Price
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Details

The AgilePulse In Vivo System provides maximum efficiency DNA vaccination delivery, intra-muscular or intra-dermal.

The AgilePulse In Vivo System used for vaccine development and gene therapy provides an intra-muscular or intra-dermal electroporation solution to produce maximum transfection efficiency.

Traditional DNA vaccine delivery systems, such as gene gun delivery, suffer from poor efficiency. The AgilePulse In Vivo System effectively introduces DNA vaccines, representing a powerful and safe means for stimulating an immune response that recognizes and eliminates target molecules in the body.

For vaccine applications, DNA vaccination through the dermal layer is preferred since it is an easily accessible site that is immunologically active. After direct injection of plasmid DNA in the dermal layer, a programmed sequence of electric pulses is applied through a miniature parallel needle electrode array to promote cellular uptake and transfection. Cells in the surrounding tissue are transfected, including dendritic antigen-presenting cells and mesenchymal origin cells. Gene expression simulates the immune system to respond to the secreted antigen. Gene expression in skin is 100-fold higher when delivery is enhanced by electroporation compared to simply injecting plasmid DNA (Roos, et. al. 2009).

The AgilePulse In Vivo system (formerly the DermaVax device) can be used for veterinary (research use only) and human tissue (research use only) applications.

The AgilePulse MAX system provides efficient transfection for up to 10 ml volumes of cell suspension.

The AgilePulse MAX System is an advanced electroporation solution for fast, efficient transfection of up to 10 ml of cell suspension. Specifically engineered for large-volume applications, our system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.

The AgilePulse MAX System is simple to use. Cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation Medium T or Cytoporation Medium T4 and transferred via sterile syringe to the large volume electroporation chamber where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes. These are followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented PulseAgile technology optimizes these pulse parameters to maximize efficiency and cell viability.

The system includes a user-friendly, programmable waveform generator with patented PulseAgile technology, our patent-pending large-volume electroporation chamber, and proprietary BTXpress Cytoporation Medium T and Cyt, optimized for large volume electroporation. The system is engineered to provide uniform electric fields in a stable temperature environment for excellent cell viability.

LARGE-VOLUME ELECTROPORATION APPLICATIONS

  • Transfect cells such as bone marrow cells to produce or replace a missing protein
  • Deliver siRNA to suppress gene expression
  • Deliver genes for permanent gene correction
  • Load cells with a drug for drug delivery
  • Cancer immunotherapy
  • Transfect eukaryotic cells for protein production in bioreactors
  • Large-scale production of replication-deficient viruses
  • CRISPR transfections

No license is required for research use of the AgilePulse MAX System but may be required for clinical and therapeutic use. Please contact BTX Technical Support for more information.

INCLUDED ITEMS

Item # Product   Included Items
47-400N AgilePulse In Vivo ID (Intra-Dermal) System (N. America)   AgilePulse ID Generator, Handle for Parallel Needle Array (47-0000), qty 3 4-needle Parallel Needle Array (47-0040), qty 3 6-needle Parallel Needle Array (47-0050), ID Software
47-40NINT AgilePulse In Vivo ID (Intra-Dermal) System     AgilePulse ID Generator, Handle for Parallel Needle Array (47-0000), qty 3 4-needle Parallel Needle Array (47-0040), qty 3 6-needle Parallel Needle Array (47-0050), ID Software
47-0401N AgilePulse In Vivo ID (Intra-Dermal) Generator Only (N. America)   AgilePulse In Vivo ID (Intra-Dermal) Generator only
47-0401INT  AgilePulse In Vivo ID (Intra-Dermal) Generator Only    AgilePulse In Vivo ID (Intra-Dermal) Generator only
47-0500N AgilePulse In Vivo IM (Intra-Muscular) System (N. America)   AgilePulse IM Generator, Handle for Parallel Needle Array 47-0000), qty 3 4-needle Parallel Needle Array (47-0045), qty 3 6-needle Parallel Needle Array (47-0070), IM Software
47-0500NINT AgilePulse In Vivo IM (Intra-Muscular) System   AgilePulse IM Generator, Handle for Parallel Needle Array 47-0000), qty 3 4-needle Parallel Needle Array (47-0045), qty 3 6-needle Parallel Needle Array (47-0070), IM Software
47-0501N AgilePulse In Vivo IM (Intra-Muscular) Generator Only (N. America)    AgilePulse In Vivo IM (Intra-Muscular) Generator Only
47-0501NINT AgilePulse In Vivo IM (Intra-Muscular) Generator Only   AgilePulse In Vivo IM (Intra-Muscular) Generator Only
47-0200N  AgilePulse MAX Large Volume Transfection System (N. America)   AgilePulse MAX Generator, Large Volume Chamber Stand, Safety Stand, 6 ml Chamber, (2X) Cytoporation Medium T, 500 ml, and 4 mm gap Cuvettes
47-0200NINT AgilePulse MAX Large Volume Transfection System   AgilePulse MAX Generator, Large Volume Chamber Stand, Safety Stand, 6 ml Chamber, (2X) Cytoporation Medium T, 500 ml, and 4 mm gap Cuvettes
47-0201N AgilePulse MAX Generator Only (N. America)    AgilePulse MAX Generator only
47-0201NINT  AgilePulse MAX Generator Only    AgilePulse MAX Generator only

AGILEPULSE ID AND IM GENERATOR SPECIFICATIONS

User Interface Touch Screen Display, Footswitch
Voltage Range 50 to 1,000 volts
Pulse Width Range 0.050 to 10 ms
Pulse Interval 0.200 to 1,000 ms (5 kHz to 1 Hz)
Data Export USB Flash Drive
Dimensions (W x H x D)
 (with handle)
32 cm  x 20 cm  x 40 cm
(12.6 in  x 7.9 in  x 15.7 in)
Weight 25 lb (11.3 kg)
Operating Temperature 10 to 40oC
Mains Voltage 100 to 250 VAC
Fuse 5 Amp Slo-Blo, 5 mm x 20 mm
Software IM (Intra-muscular) or ID (Intra-dermal)

 

AGILEPULSE MAX WAVEFORM GENERATOR SPECIFICATIONS

User Interface Touch Screen Display
Chamber Electrode Gap 1 mm, 4 mm, or 6 mm
Chamber Volume 0.5 to 10 mL
Chamber Connector Male and Female Luer Locks with Vent Filter (only for 6 mm gap chamber)
Pulse Amplitude 100 to 1,200 volts
Pulse Width 0.050 to 10 ms
Pulse Interval 0.200 to 1,000 ms (5 kHz to 1 Hz)
Data Access USB Flash Drive

AgilePulse In Vivo

  • Vaccine development  
  • Intra-dermal gene delivery
  • Intra-muscular gene delivery
  • Intra-tumor gene delivery

 

AgilePulse MAX

  • DNA transfection
  • RNA transfection
  • siRNA transfection
  • Cell loading
  • Mammalian cell transfection
  • Immunotherapy (T cell)
  • CRISPR transfections
Item # Product
47-0040 4-Needle Array, 4 mm gap, 2 mm length
47-0043 4-Needle Array, 4 mm gap, 3 mm length
47-0045 4-Needle Array, 4 mm gap, 5 mm length
47-0050 6-Needle Array, 4 mm gap, 2 mm length
47-0070 6-Needle Array, 6 mm gap, 10 mm length
47-0080 6-Needle Array, 6 mm gap, 12 mm length
47-0086 6-Needle Array, 6 mm gap, 16 mm length
47-0060 6-Needle Array, 6 mm gap, 2 mm length
47-0420 AgilePulse Foot Pedal
47-0204N AgilePulse Large Volume Chamber, 6 mm gap, 6 ml
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10
47-0203 AgilePulse MAX Safety Stand for Cuvettes
47-0209 AgilePulse MAX Safety Stand for Flatpack
47-0202N AgilePulse MAX Stand for Large Volume (6 ml) Chambers
47-0002 BTXpress Cytoporation Low Conductivity Medium T, 500 ml volume
47-0003 BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
47-0000 Needle Array Handle

AgilePulse ID User's Manual

 

AgilePulse IM User's Manual

 

AgilePulse MAX User's Manual

 

AgilePulse In Vivo System Technical Specifications

 

AgilePulse Application Note: Skin Electroporation: Effects on Transgene Expression, DNA persistence and Local Tissue Environment

 

AgilePulse Application Note: Enhancement of Cellular Immune Response to a Prostate Cancer DNA Vaccine by Intradermal Electroporation

 

AgilePulse MAX User's Manual

 

AgilePulse MAX Technical Data Sheet

 

AgilePulse MAX Application Note: Preparing clinical-grade myeloid dendritic cells by electroporation-mediated transfection

 

REFERENCES

 

Alexandre Juillerat, Alan Marechal, Jean-Marie Filhol, Julien Valton, Aymeric Duclert, Laurent Poirot, and Philippe Duchateau., Design of chimeric antigen receptors with integrated controllable transient functions. Nature Science Reports, 2016. 

 

Christopher W. Peterson, Jianbin Wang, Krystin K. Norman, Zachary K. Norgaard, Olivier Humbert, Collette K. Tse, Jenny J. Yan, Richard G. Trimble, David A. Shivak, Edward J. Rebar, Philip D. Gregory, Michael C. Holmes and Hans-Peter Kiem., Long-term multilineage engraftment of autologous genome-edited hematopoietic stem cells in nonhuman primates. Blood. 2016 127:2416-2426.

 

M. Pirouz Daftarian, Aram Vosoughi, Vance Lemmon., Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies. Methods in Molecular Biology, January 2014. 

 

Daniel X. Johansson, Karl Ljungberg, Maria Kakoulidou, Peter Liljestrom., Intradermal Electroporation of Naked Replicon RNA Elicits Strong Immune Responses. Plos one, January 4, 2012.

 

Lladser A, Ljungberg K, Tufvesson H, Tazzari M, Roos A, Quest FG, Kiessling R. Intradermal electroporation with a survivin DNA vaccine induces CTLs against a self-epitope, suppresses angiogenesis and confers long-term protection against mouse melanomaCancer Immunol Immunother. 2010 Jan; 59(1): 81-92.

 

Lundberg K, Roos A-K, Pavlenko M, Wehrum D, Pisa P. A modified epitope identified for generation and monitoring of PSA-specific T cells in patients on early phases of PSA-based immunotherapeutic protocolsVaccine. 2009 Mar 4; 27(10):1557-65.

 

Bråve A, Hallengärd D, Gudmundsdotter L, Stout R, Walters R, Wahren B, Hallermalm K. Late administration of plasmid DNA by intradermal electroporation efficiently boosts DNA-primed T and B cell responses to carcinoembryonic antigenVaccine. 2009 Jun 8; 27(28):3692-6.

 

Roos, A-K, Eriksson, F, Walters, D, Pisa, P, King, A. Optimization of skin electroporation in mice to increase tolerability of DNA vaccine delivery to patientsMolecular Therapy, 2009 Sep; 17(9):1637-42.