These Genetrodes may be used for many in vivo and in ovo electroporation applications, including drug and gene delivery. The electrodes are either inserted into a tissue or placed alongside the target tissues following injection of the molecule of interest, an electroporation pulse is delivered using a BTX Electroporation System, such as the ECM 830, ECM 2001 or Gemini X2. The electric field introduced by the Genetrodes causes transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells.
IN VIVO APPLICATIONS (using various types of Genetrodes)
The straight sharp electrode tip 5 mm or 10 mm is a general in vivo electrode. They have been used for a variety of applications. Eide et al. used the Genetrodes along with the ECM 830 to introduce plasmid DNA into free swimming embryonic Xenopus laevis.2 Dean et al. used the Genetrodes on mouse cornea, which resulted in a 30-fold higher expression than that obtained with Lipofectin.3 Murphy et al. used the Genetrodes with ECM 830 to introduce plasmid DNA into a mouse cerebellum, where he concluded that electroporation is a viable means of transferring genes to the central nervous system.
IN OVO APPLICATIONS (using the L-shaped Genetrodes)
These electrodes are ideally suited for in ovo gene transfer studies. Kos et al. used the L-Shaped Genetrodes to successfully introduce gene expression and morpholino antisense oligonucleotides in ovo1 and Paquette et al. used these L-Shaped Genetrodes to drive plasmid DNA into the neural tube of a developing egg in which widespread gene expression was observed.5
USE OF GENETRODE ELECTRODES WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Genetrode electrode. Using the ECM 830, all accessories plug directly into the unit. These electrodes can also be used with the Gemini X2 system.
|ECM 2001 System||45-0083 Coaxial Cables||45-0088 Female/Female Adapters||45-0216 Micrograbber Cables|
|Generator Compatibility||ECM 830, ECM 2001, Gemini X2|
|Voltage Range||0 - 200 VDC|
|Pulse Length Range||10 μsec – 99 msec|
|Diameter||Electrode tip 0.5 mm|
|Electrode Gap||1 - 10 mm range|
|Life Span||Approximately 1500 pulses|
- In Vivo Gene Delivery
- Ex Vivo Gene Delivery
- In Ovo Gene Delivery
|45-0087||ECM 2001 Adaptor Set, Micrograbber to Banana Plug Cables|
|45-0217||ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)|
|45-0216||ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)|
|45-0089||ECM 830 Square Post Adapters to Banana Plug Cables 45-0217|
1. Kos et al., Development, 128: 1467-1479 (2001).
2. Eide et al., FEBS Letters, 486: 29-32 (2000).
3. Dean et al., The Journal of Gene Medicine, 4: 92-100 (2002.)
4. Murphy and Messer, Molecular Therapy, 3 (1): 113-121 (2001).
5. Paquette et al., PNAS, 97: 12318-12323 (2000).