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Genetrodes Reusable Electrodes

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Genetrodes Reusable Electrodes
Needle-style reusable electrodes for in vivo, ex vivo and in ovo gene delivery
Product Item # Price  
Genetrodes, 5 mm Straight (GOLD TIP) 45-0113   View Price
Genetrodes, 10 mm Straight (GOLD TIP) 45-0114   View Price
Genetrodes, 5 mm L-Shape (GOLD TIP) 45-0115   View Price
Genetrodes, 3 mm L-Shape (GOLD TIP) 45-0116   View Price
Genetrodes, 1 mm L-Shape (GOLD TIP) 45-0117   View Price
Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables 45-0160   View Price
Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables 45-0161   View Price
Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-203 holder and 45-0216 cables 45-0162   View Price
Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables 45-0163   View Price
Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables 45-0164   View Price
Genetrode/Genepaddle Holder 45-0203   View Price
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Details

Genetrodes electrodes are needle-style reusable electrodes that are used for a variety of applications. Genetrodes come in two styles; each style comes as of a pair of electrodes. The Genetrodes styles are available as straight needle type gold tip electrodes with beveled ends or bent L-shaped electrodes with blunt ends. The Genetrodes can be positioned in parallel at a predetermined gap within a tissue using the Genetrode Holder. Genetrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Genetrodes have a life span of approximately 1500+ pulses, and are compatible with most BTX Electroporation Systems.

GENERAL APPLICATIONS

These Genetrodes may be used for many in vivo and in ovo electroporation applications, including drug and gene delivery. The electrodes are either inserted into a tissue or placed alongside the target tissues following injection of the molecule of interest, an electroporation pulse is delivered using a BTX Electroporation System, such as the ECM 830, ECM 2001 or Gemini X2. The electric field introduced by the Genetrodes causes transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells.

IN VIVO APPLICATIONS (using various types of Genetrodes)

The straight sharp electrode tip 5 mm or 10 mm is a general in vivo electrode. They have been used for a variety of applications. Eide et al. used the Genetrodes along with the ECM 830 to introduce plasmid DNA into free swimming embryonic Xenopus laevis.2 Dean et al. used the Genetrodes on mouse cornea, which resulted in a 30-fold higher expression than that obtained with Lipofectin.3 Murphy et al. used the Genetrodes with ECM 830 to introduce plasmid DNA into a mouse cerebellum, where he concluded that electroporation is a viable means of transferring genes to the central nervous system.

IN OVO APPLICATIONS (using the L-shaped Genetrodes)

These electrodes are ideally suited for in ovo gene transfer studies. Kos et al. used the L-Shaped Genetrodes to successfully introduce gene expression and morpholino antisense oligonucleotides in ovo1 and Paquette et al. used these L-Shaped Genetrodes to drive plasmid DNA into the neural tube of a developing egg in which widespread gene expression was observed.5

USE OF GENETRODE ELECTRODES WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM

In this example, the ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Genetrode electrode. Using the ECM 830, all accessories plug directly into the unit. These electrodes can also be used with the Gemini X2 system.

+ + +  
ECM 2001 System   45-0083 Coaxial Cables   45-0088 Female/Female Adapters    45-0216 Micrograbber Cables

 

Generator Compatibility ECM 830, ECM 2001, Gemini X2
Voltage Range 0 - 200 VDC
Pulse Length Range 10 μsec – 99 msec
Diameter Electrode tip 0.5 mm

 

Genetrode Holder  
Electrode Gap 1 - 10 mm range
Life Span Approximately 1500 pulses
  • In Vivo Gene Delivery
  • Ex Vivo Gene Delivery
  • In Ovo Gene Delivery
Item # Product
45-0087 ECM 2001 Adaptor Set, Micrograbber to Banana Plug Cables
45-0217 ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)
45-0216 ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)
45-0089 ECM 830 Square Post Adapters to Banana Plug Cables 45-0217
45-0203 Genetrode/Genepaddle Holder

Genetrodes User's Manual

 

Cleaning Electrodes

 

REFERENCES

 

1. Kos et al., Development, 128: 1467-1479 (2001).

 

2. Eide et al., FEBS Letters, 486: 29-32 (2000).

 

3. Dean et al., The Journal of Gene Medicine, 4: 92-100 (2002.)

 

4. Murphy and Messer, Molecular Therapy, 3 (1): 113-121 (2001).

 

5. Paquette et al., PNAS, 97: 12318-12323 (2000).