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ECM 399 Exponential Decay Wave Electroporation System

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ECM 399 Exponential Decay Wave Electroporation System
Economical electroporation system for gram negative bacteria and yeast applications
Product Item # Price  
ECM 399 Electroporation System, Complete, for Cuvettes (N. America) 45-0000   View Price
ECM 399 Electroporation System, Complete, for Cuvettes 45-0000INT   View Price
ECM 399 Generator (N. America) 45-0050   View Price
ECM 399 Generator 45-0050INT   View Price
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Details

ECM 399 GENERATOR 

The ECM® 399 is an exponential decay wave electroporaton generator specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM 399 has a limited capability for transfecting some mammalian cell lines.

The ECM 399 is ideal for basic transformation in research and academic environments.

FEATURES

  • Highest transformation efficiencies of basic bacteria and yeast strains
  • Easy to operate
  • Cost effective
  • Compact in size and portable
  • Available as complete system with cuvettes and cuvette rack

INCLUDED ITEMS

Item #ProductIncluded Items
45-0000 ECM 399 Electroporation System, Complete, for Cuvettes (N. America) ECM 399 Generator, PEP, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 660
45-0000INT ECM 399 Electroporation System, Complete, for Cuvettes  ECM 399 Generator, PEP, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 660
45-0050 ECM 399 Generator Only (N. America) ECM 399 Generator only
45-0050INT ECM 399 Generator Only ECM 399 Generator only

 

SPECIFICATIONS FOR THE ECM 399 GENERATOR

Display Type 16-character liquid crystal LCD backlit
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT I
Power  
Fusing 2.5 A, T rating 250 V
Environmental Characteristics 
Intended Use Indoor use only
Operating Temperature 10°C to 40°C
Cooling Convection through metal case
Relative Humidity 60%
Pollution Degree Not to be operated in conductive pollutants atmosphere
Altitude 2,000 m (operating)

Mechanical Characteristics 
Footprint 9.1" x 7.7" x 4.3" (W x D x H)
Weight 7.0 lb (3.2 kg)
Controls Single rotary knob with push button toggle to set parameters, On/Off power and Start switches

 

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V / 220 V Universal
Charge Time 5 sec maximum (without delay)
Voltage Range: LV Mode: 2 - 500 V / 2 V resolution; HV Mode: 10 - 2,500 V / 10 V resolution
Capacitance LV Mode: 1,050 µF fixed; HV Mode: 36 µF fixed
Resistance LV Mode: 150 ohm fixed, HV Mode: 150 ohm fixed
Maximum Voltage Output 2,500 Volts Peak
Maximum Pulse Length 125 ms at 500 V peak or 5 ms at 2,500 V peak
Safety Generator short circuit proof
  • Basic bacteria and yeast transformation
  • Mammalian transfection (limited, in low voltage mode)
Item # Product
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0212 PEP Personal Electroporation Pak Cuvette Module

ECM 399 Manual

 

ECM 399 Technical Data Sheet

 

REFERENCES

Wenjun Jiang & Ting F Zhu. Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments. Nat Protoc. May;11(5):960-75, 2016.

 

Fuwang Chen, Jie Jiang, Hongsheng OuYang, Teng Ma, Zhiyuan Peng, Yunzhi Ma, Xinrong Chen, Daxing Pang, Songyi Lin, Linzhu Ren. Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive–Negative Selectable Cassette. Applied Biochem Biotechnol. Jul; 176(5):1472-81, 2015.

 

Hila Yehuda, et al. A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance, Current Genetics. Vol. 40 pp. 282-287, 2001.

 

Taghavi et al. Electroporation of Alcaligenes eutrophus with (Mega) Plasmids and Genomic DNA FragmentsApplied and Environmental Microbiology. 60(10), pp. 3585-3591, 1994.