The Ultimate Source for all of your Electroporation and Transfection needs!

BTX, The Electroporation Experts continue to provide our customers with the technical tools, applications, protocols and references for current electroporation transfection and transformation techniques and Electrofusion applications. Electroporation is an essential method used to achieve efficient transfection of a wide range of applications including; mammalian cell lines, primary and stem cells. BTX electroporation tools are multipurpose and are widely used with to achieve efficient transfection in in vivo tissues using our in vivo electrodes. The range in vivo applications BTX can perform are numerous and include muscle, skin, brain, retina, whole organ, in utero embryos and in ovo applications. With BTX electroporation standard transformations are easily and efficiently performed in bacteria, yeast and plant cells either as a single cuvette or scale up to 96 well plates all in the same system. Electroporation and cell fusion (electrofusion) are both non-viral transfection techniques used to increase the quality of research for embryonic stem cells (ESC), adult stem cells, somatic cell nuclear transfer, therapeutic cloning, gene therapy and cancer vaccine research.

The transfection of genes into mammalian cells and silencing gene expression with siRNA are successfully performed in either a single cuvette or as a High Throughput 96 well format using the BTX HT 200 plate handler. This HT 96 well plate handler can be combined with the BTX ECM 830 Electroporation Generator to provide a simple cuvette to 96 well system. The optimal efficiency of transfection of siRNA and many other molecules are quickly achieved in our HT 96 well system without the use of viruses or chemical methods which can be toxic to cells.

BTX the electroporation experts continue to provide new products focused on improving transfection efficiencies and cell viabilities. The New BTXpress™ is the New Solution in High Performance Electroporation. A single buffer developed to quickly and efficiently deliver genes into mammalian cells that were previously considered difficult to transfect by chemical and other non-viral methods. The BTXpress™ High Performance Solution supports high efficiency transfection in numerous cell types while maintaining critical cell viability. This High Performance Electroporation buffer is equally effective at delivering DNA as well as siRNA into mammalian cells. The convenience of a single electroporation buffer in combination with our BTX electroporators make transfecting “difficult cells” an issue of the past.

The transformation of bacteria both gram negative and gram positive strains along with yeast and plant cells can be performed as either a single cuvette or in a 96 or 25 well plate. The BTX HT 100 Electroporation System which is offered with the ECM 630 Electroporation Generator can operate as either 96 well or 25 well plate formats in one system. Optimize your parameters or run large number of samples quickly with the 25 or 96 wells. This new system provides high efficiency transformations while enhancing the speed and quality of your work.

As experts in the field of Electroporation BTX helped pioneer the area of In vivo Electroporation. BTX has developed and continues to provide research scientist with a wide range of specialized electrodes for In vivo, In ovo, In utero and In vitro transfection applications. BTX offers the technical knowledge, applications, protocols and video tutorials to help make our customers successful.

In vivo electroporation and vaccine development applications including gene and drug delivery to muscle and skin tissues require our BTX straight Genetrodes, Caliper or the 2 Needle arrays while other tissues such as whole brain, brain slice, retinal, in utero embryos and other soft tissues can be transfected using either BTX Platinum, Tweezertrodes, Tissue Slice Chamber Platinum Electrode, Petri Dish Chamber Platinum Electrode, Genepaddles and Flat Plate Electrode.

The BTX L-shaped Genetrodes and platinum L-shaped Needle Electrodes are specially designed for In ovo embryo brain and neural tube tissue transfection. While explant tissue samples can be easily transfected using the Genepaddles, Petri Dish Platinum Electrodes or Platinum Tweezertrodes.

The BTX Petri Dish electrode and the Petri Pulser are designed for the electroporation of adherent cells in vitro or for larger volume electroporation transfection.

Electrofusion applications require the BTX ECM 2001 Electrofusion Generator. This instrument is specifically designed to enable higher efficiency of fusion for cell fusion, hybridoma production and plant protoplast fusion. The ECM 2001 offers a better and more efficient method to generate higher numbers of hybridoma’s or increased fused nuclear transfer embryos using the BTX electrofusion system.

The NEW MicroJect 1000 (MJ 1000) is a versatile injection system, providing the consistency and reliability to deliver precise volumes ranging from femtoliters to microliters through a stable compressed gas mechanism for a set duration of time. The MJ 1000 is designed to maximize your potential with key injection features including: Fill, Hold, Clear and Balance.