800-272-2775
Details
The Adherent Cell electrode is a reusable electroporation applicator designed to fit into each single well of a 24-well or 12-well plate.
This plate electrode is specially designed to electroporate cultured monolayers of cells on laminin-coated filters, or on 12 mm polycarbonate or polyester transwell filter units (0.4 mm pore size). It can directly electroporate adherent cells such as human corneal keratocytes, Caco-2, dental pulp cells, NC65 and BCEC and MDCK cells.
The Adherent Cell Electrode consists of two parallel plate electrodes, 7 x 19 mm with 5 mm gap spacing between the plates. Each plate electrode has a 0.3 mm height insulated foot at both ends of the bottom. When the electrode is placed in a dish, a foot minimizes the damage on cells. The high voltage cables are connected to a BTX electroporator, and the electrode is brought down onto the filter until it makes contact with the buffer, usually at 1- 2 mm above the filter. A combination of HV and LV pulses may be used. Adherent cell electrodes are compatible with Gemini X2, ECM 830, ECM 2001+, and Legacy ECM 2001 Generators.
REQUIRED CABLES
| Required for connection to ECM 830 and Gemini X2 | |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| Required for connection to ECM 2001+ | |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| 45-0088 | Female/Female Adapter Set for Banana Plug Cables |
| 45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
| Required for connection to Legacy ECM 2001 | |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| 45-0088 | Female/Female Adapter Set for Banana Plug Cables |
| 45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
USE OF ADHERENT CELL ELECTRODES WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS
In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode.
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+ |  |
| Gemini X2 System | 45-0204 Female/Female Adapters + Selected Adherent Cell Electrode |
USE OF TWEEZERTRODES WITH ECM 2001+ ELECTROFUSION AND ELECTROPORATION SYSTEM
In this example, the ECM 2001+ generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode electrode.
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+ |  |
+ |  |
+ | |
| ECM 2001+ System | 45-2057 or 45-2058 HV Output Cable | 45-0088 Female/Female Adapters | 45-0204 Tweezertrode cable Adapters + Selected Adherent Cell Electrode |
USE OF TWEEZERTRODES WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the Legacy ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Adherent Cell Electrode.
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+ |  |
+ | |
+ | |
| Legacy ECM 2001 System | 45-0083 Coaxial Cables | 45-0083 Coaxial Cables | 45-0204 Tweezertrode Cable Adapters + Selected Adherent Cell Electrode |
| Generator Compatibility | ECM 830, ECM 2001+, Legacy ECM 2001, and Gemini X2 |
| Pulse Length Range | 1 µs to 35 ms |
| Voltage Range | 0 to 300 V |
| Volume Range | 0.5 to 3.0 ml |
| Autoclavable | No |
| Field Type | Homogeneous |
| Gap Size | 5 mm |
- Mammalian Cell transfections
- Direct electroporation onto adherent cells either growing on transwell filters or coverslips, or directly onto monolayers (such as polarized epithelial) without disruption
- Plant and yeast applications
REFERENCES
1. Deora, A. et al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture, Traffic, 2007
2. BTX Protocols PR0835
