Details
The BTX Meander Fusion Chamber is a novel microslide design which is specifically used for electro cell fusion. The Meander Fusion Chamber generates a divergent field and is used for fusion of mammalian cells, plant, yeast, fungi and microorganisms.
This specialty electrode is constructed of a conductive metal alloy. It has two primary bars that are connected by many tiny fingerlike projections. These projections are spaced 0.2 mm apart. This electrode is mounted on a glass slide. It is designed for direct viewing of dimer formation during alignment while under a microscope.
The Meander Fusion Chamber is compatible with the ECM 2001+, Legacy ECM 2001, ECM 830, or Gemini X2 pulse generators. Cao et al. 1995 used the Meander Fusion Chamber to generate quadromas secreting bifunctional antibodies. He found this method both rapid and economical to produce several quadromas in a short time.
REQUIRED CABLES
Required for connection to ECM 2001+ | |
45-0087 | Micrograbber Adapter Set, Red and Black, for Banana Plug Cables |
45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
Required for connection to Gemini X2 and ECM 830 | |
45-0216 | Micrograbber to Banana Plug Connection Cables, 10 ft |
Required for connection to Legacy ECM 2001 | |
45-0216 | Micrograbber to Banana Plug Connection Cables, 10 ft |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
USE OF MEANDER FUSION CHAMBER WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001+ generator is connected using the sequence of cables and adapters illustrated below, then connected to the Meander Fusion Chamber.
+ | + | |||
ECM 2001+ System | 45-0087 Micrograbber Adapters + Meander Fusion Chamber |
USE OF MEANDER FUSION CHAMBER WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS
In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Meander Fusion Chamber.
+ | |||
Gemini X2 System | 45-0216 Micrograbber Cables + Meander Fusion Chamber |
USE OF THE MEANDER FUSION CHAMBER WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the Legacy ECM 2001 generator is connected to the chamber using the sequence of cables and adapters illustrated below.
+ | + | + | ||||
Legacy ECM 2001 System | 45-0083 Coaxial Cables | 45-0088 Female/Female Adapters | 45-0216 Micrograbber Cables + Meander Fusion Chamber |
Generator Compatibility | ECM 2001+, Legacy ECM 2001, ECM 830, and Gemini X2 |
Field Type | Divergent |
Max Voltage: | |
AC | 16 V (0 - peak) |
DC | 0 to 480 V |
Pulse Length | 1 µsec to 99 msec |
Number of Pulses | 1 to 99 (depending on voltage) |
Gap Size | 0.2 mm |
Autoclavable | No |
- Cell Fusion
- Plant Protoplast Fusion
Item # | Product |
---|---|
45-0106 | Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1 |
Meander Fusion Chamber User's Manual
REFERENCES
Cao et al. A rapid non-selective method to generate quadromas by microelectrofusion. Journal of Immunological Methods. 187: 1-7 (1995).
Von Keller et al. Intracelluar movements and reorganization of electrically fused sunflower protoplasts. Int. J. Plant Sci. 156(6): 764-773 (1995).