The BTX Caliper Electrodes are in vivo style reusable electrodes used for a variety of applications. Caliper Electrodes are available in two sizes. Each size consists of a caliper and a pair of electrode plates. The 1.0 x 1.0 cm electrode is used in smaller animals with a smaller tissue surface area, while the 2.0 x 2.0 cm is used to target slightly larger surface areas. The end plates of the Caliper Electrode may be adjusted by using the black roller mounted on the caliper. Caliper Electrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Caliper Electrodes have an unlimited lifetime, and are compatible with the ECM 830, ECM 2001 and Gemini X2 Electroporation Systems.
Caliper Electrodes may be used for many electroporation applications, including drug and gene delivery. The electrodes span the target area and deliver electroporation pulses following injection of the molecule of interest. The electric field introduced by the Caliper Electrodes cause transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells. Prud'Homme et al. used the Caliper electrodes to deliver plasmid DNA to skin for gene therapy studies on diabetes.1 Mir et al. used the Caliper Electrode for in vivo skeletal muscle work2 as well as subcutaneous tumor electrochemotherapy applications3. Caliper Electrodes can potentially be used for intact plant work as well.4
USE OF CALIPER ELECTRODES WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below, then connected to the caliper electrode. Using the ECM 830, all accessories plug directly into the unit.
|ECM 2001 System||45-0083 Coaxial Cables||45-0088 Female/Female Adapters|
|Generator Compatibility||ECM 830, ECM 2001 and Gemini X2|
|Voltage Range||0 - 500 V (depending on electrode gap)|
|Pulse Length Range||10 µsec - 99 msec|
|Electrode Gap||0.1 - 13 cm|
|Electrode Dimensions||1 x 1 cm (brass) or 1.5 x 1.5 cm and 2 x 2 cm (stainless steel)|
- In vivo drug or gene delivery
- Transdermal tissue applications
- Intact plant applications
1. Prud'Homme et al., Human Gene Therapy, 13: 395-406 (2002).
2. Mir et al., PNAS, 96, 4262-4267 (1999).
3. Mir et al., British Journal of Cancer, 77(12), 2336-2342 (1998).
4. Dev et al., U.S. Patent #5,859,327 Jan 12, 1999.
5. Hartikka et al., Molecular Therapy, 4(5), 407-415 (2001).