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Gemini Twin Wave Electroporators

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Gemini Twin Wave Electroporators

Ideal for CRISPR
With NEW LARGE 7.0-IN TOUCH SCREEN!


Flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more.


Product Item # Price  
Gemini X2 Electroporation System Complete, for Cuvettes 45-2040   View Price
Gemini X2 Electroporation Generator Only 45-2041   View Price
Gemini SC Electroporation System Complete, for Cuvettes 45-2042   View Price
Gemini SC Electroporation Generator Only 45-2043   View Price
Gemini X2 High Throughput Electroporation System, 96-well with HT-200 45-2044   View Price
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Details

The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.

These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.

FEATURES & BENEFITS

  • Square wave and exponential decay waves power in a single unit
  • Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
  • Large, easy-to-use touch screen interface
  • Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
  • Preset protocols – includes the most common eukaryotic and prokaryotic cell types
  • User-defined protocols – unlimited ability to add and modify protocols
  • Safety – displays resistance measurements for each pulse with three layers of arc protection
  • Data management – stores logs of every pulse delivered for QC and troubleshooting

GEMINI SC

The Gemini SC system is designed specifically for cuvette-based in vitro transfections of eukaryotic or prokaryotic cells in suspension. With a wide range of pulsing parameters, advanced safety features as well as dozens of preset protocols, the Gemini SC is ideal for any lab requiring efficient cell transfection or transformation without the use of costly reagents.

GEMINI X2 AND GEMINI X2 HT

The Gemini X2 system provides the ultimate experimental flexibility. This one easy setup allows for transfections in cuvettes or high throughput plates, or the capability to utilize the wide variety of BTX specialty electrodes for specific applications. In addition, Gemini X2 electrical output specifications are wide-ranging, making it the most versatile electroporation system available today.

Electroporation of suspension cells can be achieved in cuvettes (X2) or 96-well plates (X2 HT) with an HT plate handler. Additionally, the Gemini X2 can be paired with BTX specialty electrodes to deliver gene and drugs in vivo, in utero, in ovo and to explant tissues as well as adherent cells.


GEMINI'S BROAD CAPABILITIES

Exponential Decay/Square Twin Waveforms

Allows researchers to select which type of waveform they would like to use to transfect eukaryotic cells or transform prokaryotic cells. Typically, eukaryotic cells respond best to square wave pulses while prokaryotic cell respond well to exponential decay wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their application.

Simple User Interface
Permits intuitive touch screen or computer-controlled protocol programing and execution as well as password protection of protocols.Each system is programmable and offers unlimited protocol storage as well as dozens of proven pre-set protocols to reduce optimization time. The X2 systems provide protocol delivery log storage. Logs can be accessed for quality control, optimization or troubleshooting purposes.

Pre-Pulse Resistance Measurement
Offers assurance that sample resistance is within acceptable range for electroporation. This feature protects precious sample as well as system component to safeguard against arcing.

Widest Voltage/Pulse length Range Available
Provides researchers with the widest range of voltage options available in the market.

  • The Gemini X2 can achieve voltage delivery as low as 5 V and as high as 3000 V in both waveforms in addition to pulse lengths of 10 µs to 5 s, from delicate in ovo tissue to hearty gram positive bacteria.
  • The Gemini SC can achieve voltage delivery as low as 10 V and as high as 3000 V in both waveforms in addition to pulse lengths of 50 µs to 5 s.

Highly Accurate Pulsing
Delivers precise voltage, pulse length/time constant, pulse interval and displays the values back to the researcher allowing for visual confirmation of successful electroporation.

Revolutionary Arc Proofing Safety Features
Gives researchers peace of mind knowing that their sample and their systems are safe from troublesome arcing with enhanced 4-way safety checks through the pulsing procedure. Using two separate pre pulse, sample resistance monitoring scans to ensure proper conditions, as well as internal safety features auditing pulse progress in at the component as well as software level, arcing is dramatically decreased.

The Gemini is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.


INCLUDED ITEMS

Item #DescriptionIncluded Items
45-2040 Gemini X2 Electroporation System Complete for Cuvettes Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack 660
45-2041 Gemini X2 Electroporation Generator Only Gemini X2 Electroporation Generator only
45-2042 Gemini SC Electroporation System Complete for Cuvettes Gemini SC Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. 30 (10 each), and Cuvette Rack 660
45-2043 Gemini SC Electroporation Generator Only Gemini SC Electroporation Generator only
45-2044 Gemini X2 High Throughput System, 96-well with HT-200 Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and cuvette Rack 660, HT-200 Plate Handler, 2 mm gap HT plate and 4 mm gap HT plate

Gemini X2

Gemini SC

Other Specifications

 

 GEMINI X2 SPECIFICATIONS

SQUARE WAVE PULSE RANGES 
Voltage Range 
  LV Mode 5 to 500 V in 1 V steps
  HV Mode 505 to 3000 V in 5 V steps
Voltage Accuracy 
  LV Mode 5%
  HV Mode 5%
Pulse Length Range 
  LV Mode 10 μs to 999 μs in 1 μs steps
1 ms to 999 ms in 1 ms steps
  HV Mode 10 μs to 600 μs in 1 μs steps
Multiple Pulsing 
  Single Samples 1 to 99 pulses per sample
  HT Plate Handler Mode 1 to 10 pulses per sample (up to 120 total)
  Pulse Interval 0.1 s to 10 s
Capacitance 
  LV Mode 3775 μF
  HV Mode 85 μF
  Voltage Droop 20% Load <1 K Ω, 10% Load >1 K Ω
EXPONENTIAL DECAY PULSE RANGES 
Voltage Range  
  LV Mode 5 to 500 in 1 V steps
  HV Mode 505 to 3000 in 5 V steps
Voltage Accuracy 
  LV Mode 5%
  HV Mode 5%
Time Constant Range 
  LV Mode 1 ms to 5.158 s
  HV Mode 0.5 ms to 133.875 ms
Multiple Pulsing 
  Multiple Pulsing 1 to 2 depending on device's current amplitude limit
  Pulse Interval 5 to 30 s
Capacitance 
  LV Mode 25 to 3275 μF in 25 μF steps
  HV Mode 10, 25, 35, 50, 60, 75, 85 μF
Available Internal Resistance Selections 
  All Modes LV 25 to 1575 Ω in 25 Ω steps
HV 50 to1575 Ω in 25 Ω steps
SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE 
LV Mode PL ≤100 ms, load ≥8 to 9 Ω, PL >100 ms, load ≥100 Ω
HV Mode Load ≥40 Ω

 

 GEMINI SC SPECIFICATIONS

SQUARE WAVE PULSE RANGES 
Voltage Range 
  LV Mode 10 to 500 V in 5 V steps
  HV Mode 510 to 3000 V in 10 V steps
Voltage Accuracy 
  LV Mode 5%
  HV Mode 5%
Pulse Length Range 
  LV Mode 0.05 ms to 10 ms in 0.05 ms steps
10 ms to 100 ms in 1 ms steps
  HV Mode 50 μs to 5 ms in 50 μs steps
Multiple Pulsing 
  LV Mode 1 to 10 pulses per sample
  HV Mode 1 to 2 pulses per sample
  Pulse Interval  0.1 s to 10 s
Capacitance 
  LV Mode 3775 μF
  HV Mode 85 μF
  Voltage Droop 20% Load <1 K Ω, 10% Load >1 K Ω
EXPONENTIAL DECAY PULSE RANGES 
Voltage Range 
  LV Mode 10 to 500 V in 5 V steps
  HV Mode 510 to 3000 V in 10 V steps
Voltage Accuracy 
  LV Mode 5%
  HV Mode 5%
Time Constant Range 
  LV Mode 1.25 ms to 3.275 s / 1.25 ms
  HV Mode 0.5 ms to 50 ms / 0.5 ms
Multiple Pulsing 
  Multiple Pulsing No
  Pulse Interval N/A
Capacitance 
  LV Mode 25 to 3275 μF in 25 μF steps
  HV Mode 10, 25 and 50 μF
Available Internal Resistance Selections 
  All Modes 50 to 1000 Ω in 50 Ω steps
SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE 
  LV Mode Load ≥10 Ω
  HV Mode 510 - 2500 V Load ≥20 Ω, 2500 - 3000 V, Load  ≥600 Ω

 

OTHER SPECIFICATIONS (X2 AND SC)

Charging Time LV <7 s, HV <4 s
Monitoring Pulse Voltage, Width, Droop % and Sample Resistance
Display 7.0-in color display
Controls Touch Screen
Programmability Storage over 1,000 Protocols
Arc Control Yes
Interlock Switches Yes
Pre-Pulse Sample Resistance Check Yes
Pulse Over Current Protection Yes
PC Control X2: Yes; SC: No
PC Communications X2: Yes; SC: No
Log Report X2: Yes; SC: No
Remote Operation X2: Yes; SC: No
Foot Switch Control X2: Yes; SC: No
Power Ratings 35 W idle and 350 W pulsing
Input Voltage Ratings 100 to 240 VAC
CE, ETL Marking, CB Scheme, EuRoHS Yes
Dimensions (H x W x D) 12.75 x 11.25 x 8.5
Weight ~16 lb
Warranty 2 years
Atmospheric Specifications  
  Operating Temperature 4˚C to 40˚C (40˚F to 104˚F)
  Storage Temperature -10˚C to 70˚C (14˚F to 158˚F)
  Storage Humidity 20% to 80% RH, non condensing
Mode of Operation Continuous
Classification Class I
Pollution Degree I
Installation Category II
Regulatory Certifications CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme
  • CRISPR transfections
  • Suspension and adherent cells
  • In vivo, in ovo and in utero gene and drug delivery
  • Tissue explants
  • Transformation of eukaryotic and prokaryotic cells
  • High throughput electroporation
Item # Product
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0466 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, coated, 1 plate
45-0463 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, pkg. of 6
45-0467 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, pkg. of 6
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0462 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, 1 plate
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, Coated, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 250 µl, 1 plate
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
45-0101 Caliper Electrode , 1.0 cm X 1.0 cm, Brass, Transdermal Delivery Applications
45-0102 Caliper Electrode, 2.0 cm x 2.0 cm with extra 1.5 cm x 1.5 cm electrodes included, Stainless Steel, Electrochemotherapy Applications
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125INT Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0059INT Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables
45-0059 Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America)
45-0108 Flat Electrode
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-2030 Gemini X2 Foot Pedal
45-0169 Genepaddles Kit, 3 x 5 mm (includes 45-0203 holder and 45-0216 cables)
45-0170 Genepaddles Kit, 5 x 7 mm (includes 45-0203 holder and 45-0216 cables)
45-0122 Genepaddles, 3 x 5 mm (need 45-0203 holder and 45-0216 cables)
45-0123 Genepaddles, 5 x 7 mm (need 45-0203 holder and 45-0216 cables)
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0400INT HT 100 Plate Handler, Manual Track Switching
45-0400 HT 100 Plate Handler, Manual Track Switching (N. America)
45-0401INT HT-200 Plate Handler, Auto-Sense Track Switching
45-0401 HT-200 Plate Handler, Auto-Sense Track Switching (N. America)
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0100 Petri Dish Electrode
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0130 Petri Pulser
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-2020 Safety Dome for the Gemini SC
45-2021 Safety Dome for the Gemini X2
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

Gemini Datasheet

 

CRISPR Brochure

 

Gemini Downloads and Firmware Updates

 

REFERENCES

Kuo CY, Lin CH, Hsu T. VHL inactivation in precancerous kidney cells induces an inflammatory response via ER stress-activated IRE1α signaling. Cancer Res. 2017 Jul;77: 3406-3416.

 

Lopes A, Vanvarenberg K, Preat V, Vandermeulen G. Codon-Optimized P1A-Encoding DNA Vaccine: Toward a Therapeutic Vaccination against P815 Mastocytoma. Mol TherNucleic Acids.2017 Sep;8: 404-415, 2017.

 

Seyed Jafari SM, et al. Efficacy of In Vivo Electroporation-Mediated IL-10 Gene Delivery on Survival of Skin Flaps. J Membr Biol. 2017 Aug. doi: 10.1007/s00232-017-9974-x. [Epub ahead of print]

 

Sun, L. et al. Glutamine is required for snakehead fish vesiculovirus propagation via replenishing the tricarboxylic acid cycleJ Gen Virol, 2016 Nov;97(11): 2489-2855. 

 

Wang, Y. et al. Autophagy induced by snakehead fish vesiculovirus inhibited its replication in SSN-1 cell lineFish Shellfish Immunol. 2016 Aug;55: 415-422.

 

Stanga, S. et al. APP-dependent glial cell line-derived neurotrophic factor gene expression drives neuromuscular junction formationFASEB J. 2016 May;30(5): 1696-1711. 

 

Cameron, DE, Collins, JJ. Tunable protein degradation in bacteriaNat. Biotech. 2014 Dec;32(12): 1276-1281.