800-272-2775
Details
The ECM 399 is an economical electroporation system for gram negative bacteria and yeast applications. Ideal for research and academic labs.
ECM 399 GENERATOR
The ECM 399 is an exponential decay wave electroporaton generator specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM 399 has a limited capability for transfecting some mammalian cell lines.
The ECM 399 is ideal for basic transformation in research and academic environments.
This instrument is intended for Research and Pre-Clinical use only and is not for use in diagnostic or clinical procedures.
FEATURES
- Highest transformation efficiencies of basic bacteria and yeast strains
- Easy to operate
- Cost effective
- Compact in size and portable
- Available as complete system with cuvettes and cuvette rack
INCLUDED ITEMS
| Item # | Product | Included Items |
|---|---|---|
| 45-0000 | ECM 399 Electroporation System, Complete, for Cuvettes | ECM 399 Generator, PEP, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 660 |
| 45-0050 | ECM 399 Generator Only | ECM 399 Generator only |
SPECIFICATIONS FOR THE ECM 399 GENERATOR
| Display Type | 16-character liquid crystal LCD backlit |
| Power Source Voltage | 100 to 240 VAC, 50 to 60 Hz, CAT I |
| Power | |
| Fusing | 2.5 A, T rating 250 V |
| Environmental Characteristics | |
| Intended Use | For research use only |
| Operating Temperature | 10°C to 40°C |
| Cooling | Convection through metal case |
| Relative Humidity | 60% |
| Pollution Degree | Not to be operated in conductive pollutants atmosphere |
| Altitude | 2,000 m (operating) |
| Mechanical Characteristics | |
| Footprint | 9.1" x 7.7" x 4.3" (W x D x H) |
| Weight | 7.0 lb (3.2 kg) |
| Controls | Single rotary knob with push button toggle to set parameters, On/Off power and Start switches |
STANDARD CAPABILITIES
| Operational Status | Internal self test upon start-up |
| Interface | Digital User Interface |
| Input | 110 V / 220 V Universal |
| Charge Time | 5 sec maximum (without delay) |
| Voltage Range: | LV Mode: 2 - 500 V / 2 V resolution; HV Mode: 10 - 2,500 V / 10 V resolution |
| Capacitance | LV Mode: 1,050 µF fixed; HV Mode: 36 µF fixed |
| Resistance | LV Mode: 150 ohm fixed, HV Mode: 150 ohm fixed |
| Maximum Voltage Output | 2,500 Volts Peak |
| Maximum Pulse Length | 125 ms at 500 V peak or 5 ms at 2,500 V peak |
| Safety | Generator short circuit proof |
- Basic bacteria and yeast transformation
- Mammalian transfection (limited, in low voltage mode)
| Item # | Product |
|---|---|
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0212 | PEP Personal Electroporation Pak Cuvette Module |
REFERENCES
Wenjun Jiang & Ting F Zhu. Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments. Nat Protoc. May;11(5):960-75, 2016.
Fuwang Chen, Jie Jiang, Hongsheng OuYang, Teng Ma, Zhiyuan Peng, Yunzhi Ma, Xinrong Chen, Daxing Pang, Songyi Lin, Linzhu Ren. Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive–Negative Selectable Cassette. Applied Biochem Biotechnol. Jul; 176(5):1472-81, 2015.
