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Details
The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.
ECM 2001 GENERATOR
The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
WAVEFORMS
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.
FEATURES & BENEFITS
- AC waveform of 0.2 – 2.0 MHz
- Square wave electroporation capabilities
- A wide range of voltages from 5 V to 3000 V
- Fine voltage discrimination
- Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
- Capable of operating at low impedance loads
- Large touchscreen interface
APPLICATIONS
ELECTROFUSION
Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC sine wave and DC square wave pulses. Fusion is achieved by the generation of an AC current sine waveform that generates a benign dielectrophoretic alignment of cells. Next, a DC square waveform is applied to promote fusion between aligned cells. After fusion, the AC sine waveform is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.
ELECTROPORATION
Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used in transient transfections to study protein expression or to temporarily knockout or “silence” these genes using siRNA. This is used to study gene targeting and function.
Square waveform electroporation is an efficient non-viral method for transfecting genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and to understand protein regulation.
Alternatively, adding additional selection steps to isolate stably transfected cells allows for ;integration of a gene into the genome of the cell for long term expression of protein. The use of the ECM 2001+ offers the control needed to adjust electrical settings for optimization of parameters. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
PRODUCT CONFIGURATIONS BY APPLICATION
Complete Cell Fusion and Electroporation System
For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001+ Cell Fusion System complete (45-2045) is our most versatile instrument configuration. The ECM 2001+ Cell Fusion system complete comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.
Hybridoma Production
For use in large volume cell fusion applications such as hybridoma production, the ECM 2001+ generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. This system (45-2048) does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.
Embryo Manipulation
For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001+ Embryo Manipulation System (45-2047). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.
Mammalian Cell Transfections
Utilizing the ECM 2001+ System Complete, for Cuvettes (45-2046) for standard mammalian cell transfections is simple. The AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 10 pulses per experiment, make this an extremely versatile system for any lab.
Adherent Cell Transfections
Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001+ coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.
INCLUDED ITEMS
| Item # | Description | Included Items |
|---|---|---|
| 45-2045 | ECM 2001+ Cell Fusion System Complete | ECM 2001+ Generator, Microslides (0.5 mm gap, 3.2 mm gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-2046 | ECM 2001+ Electroporation System Complete, for Cuvettes | ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-2047 | ECM 2001+ Embryo Manipulation System | ECM 2001 Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables |
| 45-2048 | ECM 2001+ Hybridoma System | ECM 2001+ Generator, 2 ml Coaxial Optimization Chamber (470030), 9 ml Coaxial Production Chamber, Female/Female Adapter Set, Cytofusion Medium C (500 ml) |
| 45-2049 | ECM 2001+ Generator Only | ECM 2001+ Generator, 5 ft High Voltage Output Cable, F/F adapter set |
| 45-2050 | ECM 2001+ System Complete, for Cuvettes with Monitoring | ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), Cuvette Rack, Enhancer 3000 Probe, Enhancer Interface Box, Oscilloscope and Cables |
| Square Wave Pulse, DC | |
| Voltage Range | |
| LV Mode | 5 to 500 V in 1 V steps |
| HV Mode | 505 to 3000 V in 1 V steps |
| Voltage Accuracy | |
| LV Mode | 5% |
| HV Mode | 5% |
| Pulse Length Range | |
| LV Mode | 10 to 999 µs in 1 µs steps |
| LV Mode | 1 to 999 ms in 1 ms steps |
| HV Mode | 10 to 600 µs in 1 µs steps |
| Multiple Pulsing | |
| LV Mode | 1 to 10 pulses per sample |
| HV Mode | 1 to 10 pulses per sample |
| Pulse Interval | 0.1 s to 10 s |
| Alignment (Pre-Pulse), AC | |
| Frequency | 0.2 to 2 MHz in 0.1 MHz steps |
| Voltage | 5 to 75 V in 5 V steps |
| Duration | 0 to 99 s in 1 s steps |
| Wave Shape | Sine wave |
| Post-Fusion, AC | |
| Frequency | 0.2 to 2 MHz in 0.1 MHz steps |
| Voltage | 5 to 75 V in 5 V steps |
| Duration | 0 to 99 s in 1 s steps |
| Wave Shape | Sine wave |
| Sample Load Ranges | |
| AC | |
| All Voltages | Load must be ≥60 Ω> |
| DC | |
| LV Mode | Pulse Length <100 ms, Load must be >8 to 9 Ω, Pulse Length >100 ms, Load must be >100 Ω |
| HV Mode | Pulse Length ≤600 ms Load must be ≥40 Ω |
| Other Specifications | |
| Charging Time | LV = 7 s, HV = 7 s |
| DC Monitoring | Protocol Name, Steps Executed, Time/Date, Error Conditions, Pulse Voltage, Pulse Current, Pulse Width, Pulse #, Droop %, Sample Voltage, Sample Current and Sample Resistance |
| Display | 7-inch full color display |
| Controls | Touch Screen |
| Programmability | Stores over 1000 protocols |
| DC Arc Control | Yes |
| Pre-Pulse Sample Load Check | Ves (20 V DC) |
| Computer (Optional) | PC running Microsoft Windows XP, 7, 8.1 or 10 |
| Communications | USB |
| Data File Format | Comma Separated Values (CSV) |
| Remote Operation | Yes, using Foot Pedal control |
| Accessory Connector Type | Coaxial |
| Power Ratings | 70 W idle and 900 W pulsing |
| Input Voltage Ratings | 100 to 240 VAC, 50 / 60 Hz |
| Dimensions (H x W x D) | 13 x 12 x 13 in |
| Weight | 22 lb |
| Warranty | 2 years |
| Atmospheric Specifications | |
| Operating Temperature | 4°C to 40°C (40°F to 104°F) |
| Storage Temperature | -10°C to 70°C (14°F to 158°F) |
| Operating Humidity | 20% to 80% RH, non-condensing |
| Storage Humidity | 20% to 80% RH, non-condensing |
| Mode of Operation | Continuous |
| Classification | Class I |
| Pollution | IP2X |
| Installation | Category II |
| Regulatory Certifications | CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme |
- Cell fusion
- Hybridoma production
- Nuclear transfer
- Embryo manipulation
- Mammalian cell transfection
- Plant protoplast fusion
- Stem cell production
| Item # | Product |
|---|---|
| 45-0167 | 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 45-0531 | Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable |
| 45-0530 | Adherent Cell Electrode, 5 mm gap |
| 47-0206 | Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10 |
| 47-0001 | BTXpress Cytofusion Medium C, 500 ml volume |
| 47-0003 | BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume |
| 47-0020 | 9 ml Coaxial Chamber for production |
| 47-0030 | Hybrimune 2 ml Coaxial Chamber for optimization |
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0087 | ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables |
| 45-0217 | ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode) |
| 45-0086 | Legacy ECM 2001 CE Footswitch with 45-0085 cable |
| 45-0088 | ECM 2001 Female/Female Adapter Set for Banana Plug Cables (Use with 45-0216) |
| 45-0214 | ECM 2001 Footswitch (for non-CE 2001 only) |
| 45-0085 | ECM 2001 Footswitch Cable |
| 45-0216 | ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish) |
| 47-0208 | ECM 2001 Safety Stand for Flatpack |
| 45-0059 | Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America) |
| 45-0108 | Flat Electrode |
| 45-0110 | Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50 |
| 45-0109 | Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50 |
| 45-0161 | Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0162 | Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0160 | Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables |
| 45-0117 | Genetrodes, 1 mm L-Shape (GOLD TIP) |
| 45-0164 | Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0114 | Genetrodes, 10 mm Straight (GOLD TIP) |
| 45-0116 | Genetrodes, 3 mm L-Shape (GOLD TIP) |
| 45-0163 | Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0115 | Genetrodes, 5 mm L-Shape (GOLD TIP) |
| 45-0113 | Genetrodes, 5 mm Straight (GOLD TIP) |
| 45-0106 | Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1 |
| 45-0105 | Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1 |
| 45-0103 | Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10 |
| 45-0104 | Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10 |
| 45-0495 | Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only) |
| 45-0496 | Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables) |
| 45-0100 | Petri Dish Electrode |
| 45-0501 | Petri Dish Platinum Electrode Chamber, 10 mm, negative |
| 45-0506 | Petri Dish Platinum Electrode Chamber, 15 mm gap |
| 45-0504 | Petri Dish Platinum Electrode Chamber, 5 mm gap |
| 45-0491 | Petri Dish Platinum Electrode Chamber, 7 mm, negative |
| 45-0507 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap |
| 45-0505 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap |
| 45-0513 | Petri Dish Tissue Chamber Kit, 15 mm gap with cables |
| 45-0130 | Petri Pulser |
| 45-0525 | Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable) |
| 45-0486 | Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables) |
| 45-0524 | Platinum Tweezertrode, 1 mm Flat |
| 45-0487 | Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable) |
| 45-0489 | Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables) |
| 45-0488 | Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables) |
| 45-0207 | Safety Stand, Adjustable Gap (for use with Cuvettes) |
| 45-0119 | Stainless Tweezertrode Electrode, 10 mm Diameter |
| 45-0118 | Stainless Tweezertrode Electrode, 7 mm Diameter |
| 45-0166 | Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable) |
| 45-0165 | Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable) |
| 45-0493 | Triple Electrode Tweezertrode, 3 mm |
| 45-0494 | Triple Electrode Tweezertrode, 5 mm |
Cell Fusion Applications: Electrofusion vs. PEG
REFERENCES
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