The AgilePulse™ PLUS Bipolar Transfection System is designed for efficient transfection of mammalian cells and tissues. This system is ideal for high efficiency transfection of mammalian cells, including difficult to transfect cell types, such as stem cells and primary cells. It is also suitable for scale up to large volume electroporation for applications such as large-scale creation of engineered cells and large-scale protein expression.
The AgilePulse PLUS allows the user to incorporate polarity switching into electroporation protocols, thus enabling the user to minimize accumulation of transfectant at a single electrode and maximizing delivery of transfectant. The system is designed to be easy to set-up and to operate in a research environment.
The AgilePulse PLUS Waveform Generator can transfect cells suspended in cuvettes, flatpacks or large volume chambers. Additionally, it can transfect tissues with BTX in vivo electrodes. The system offers the flexibility to be used in combination with BTX specialty electrodes for in vivo and adherent cell electroporation.
- Program up to 5 groups of pulses with separately defined pulse number, voltage, pulse length, interval, and polarity—within each group.
- Direct scale-up from standard low-volume cuvettes to large-volume chambers— transfect up to a billion cells in suspension per sample.
- Incorporate bipolar switching into your electroporation protocols for convenient, more efficient in vivo transfection protocols—pulse chains in each group may be defined as +, -,
or flip (+ —> - or - —> +) polarity.
- Maximum number of pulses per protocol increases to 100 pulses.
- Compatible with all BTX specialty electrodes for adherent cell electroporation, intradermal electroporation, intramuscular electroporation and other in vivo transfection applications.
|Item #||Product||Included Items|
AgilePulse PLUS Bipolar
|AgilePulse PLUS Generator, 5-ft HV Output Cable, F/F Adapters, Extended
Capacity Safety Stand, Cytoporation Medium T, 4 mm Gap Cuvettes and
AgilePulse PLUS Bipolar
|AgilePulse PLUS Generator, 5-ft HV Output Cable and F/F Adapters|
Additional accessories such as Large Volume Chamber Stand and Needle Array Electrodes are available separately.
|Pulse Program Specifications|
|Voltage Range||100 to 1200 VDC|
|Pulse Width Range||0.05 to 10 ms|
|Pulse Interval Range||0.2 to 1000 ms|
|Number of Pulses per Group||1 to 10|
|Group Interval Range||0.2 to 1000 ms|
|Polarity Settings for Each Group||Positive, Negative, Flip Positive → Negative, or Flip Negative → Positive|
|Number of Pulse Groups||1 to 5|
|User Interface||Capacitive Touchscreen Display|
|Data Export*||USB thumb drive|
|Input Voltage and Frequency Ratings||100 to 240 VAC, 50/60 Hz|
|Storage Temperature||-10°C to 70°C (14°F to 158°F)|
|Operating Temperature||4°C to 40°C (40°F to 104°F)|
|Intended Use||Indoor Use Only|
|Operating/Storage Humidity||20% to 80% RH, non-condensing|
|Insulation Category||Category II|
|Regulatory Certifications||CE, ETL (UL, CSA), FCC, WEEE, EU and RoHS|
|Dimensions, W x H x D||30.5 x 7.8 x 5.4 cm (12 x 6.25 x 14.5 in)|
|Weight||5.7 kg (12.5 lb)|
| * All data automatically stored in internal memory and may be downloaded to external USB Key. Maximum data logs
stored and retrievable from internal memory >20,000.
- Suspension and adherent cell transfection
- Large volume electroporation
- CAR-T cell production
- CRISPR gene editing
- Intradermal, intramuscular and intratumor delivery
- Animal immunization and vaccine research
|47-0610N||AgilePulse PLUS Safety Stand for Flatpack|
|47-0620N||AgilePulse PLUS Large Volume Chamber Stand for Large Volume 5 ml Chambers|
|47-0095||AgilePulse PLUS HV Output Cable, 5 ft|
|47-0096||AgilePulse PLUS HV Output Cable, 10 ft|
|45-0088||Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables|
|45-0126||Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow|
|45-0206||2-Needle Array Handle, 5 mm, for 45-0168|
|47-0204N||AgilePulse Large Volume Chamber, 6 mm gap, 5 ml, 2 ports w/fittings|
|45-0168||2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle|
|47-0097||Adapter, connects AgilePulse PLUS to AgilePulse MAX Safety Stands and Large Volume Chamber Stands|
|47-0098||Adapter, connects AgilePulse PLUS to AgilePulse In Vivo Needle Array Handles|
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