800-272-2775
Details
The AgilePulse™ PLUS Bipolar Transfection System is designed for efficient transfection of mammalian cells and tissues. This system is ideal for high efficiency transfection of mammalian cells, including difficult to transfect cell types, such as stem cells and primary cells. It is also suitable for scale up to large volume electroporation for applications such as large-scale creation of engineered cells and large-scale protein expression.
The AgilePulse PLUS allows the user to incorporate polarity switching into electroporation protocols, thus enabling the user to minimize accumulation of transfectant at a single electrode and maximizing delivery of transfectant. The system is designed to be easy to set-up and to operate in a research environment.
The AgilePulse PLUS Waveform Generator can transfect cells suspended in cuvettes, flatpacks or large volume chambers. Additionally, it can transfect tissues with BTX in vivo electrodes. The system offers the flexibility to be used in combination with BTX specialty electrodes for in vivo and adherent cell electroporation.
Features
- Program up to 5 groups of pulses with separately defined pulse number, voltage, pulse length, interval, and polarity—within each group.
- Direct scale-up from standard low-volume cuvettes to large-volume chambers— transfect up to a billion cells in suspension per sample.
- Incorporate bipolar switching into your electroporation protocols for convenient, more efficient in vivo transfection protocols—pulse chains in each group may be defined as +, -,
or flip (+ —> - or - —> +) polarity. - Maximum number of pulses per protocol increases to 100 pulses.
- Compatible with all BTX specialty electrodes for adherent cell electroporation, intradermal electroporation, intramuscular electroporation and other in vivo transfection applications.
Included Items
| Item # | Product | Included Items | |
| 47-0600N |
AgilePulse PLUS Bipolar |
AgilePulse PLUS Generator, 5-ft HV Output Cable, F/F Adapters, Extended Capacity Safety Stand, Cytoporation Medium T, 4 mm Gap Cuvettes and Cuvette Rack |
|
| 47-0601N |
AgilePulse PLUS Bipolar |
AgilePulse PLUS Generator, 5-ft HV Output Cable and F/F Adapters |
Additional Accessories
Additional accessories such as Large Volume Chamber Stand and Needle Array Electrodes are available separately.
| Pulse Program Specifications | |
| Voltage Range | 100 to 1200 VDC |
| Pulse Width Range | 0.05 to 10 ms |
| Pulse Interval Range | 0.2 to 1000 ms |
| Number of Pulses per Group | 1 to 10 |
| Group Interval Range | 0.2 to 1000 ms |
| Polarity Settings for Each Group | Positive, Negative, Flip Positive → Negative, or Flip Negative → Positive |
| Number of Pulse Groups | 1 to 5 |
| Other Specifications | |
| User Interface | Capacitive Touchscreen Display |
| Data Export* | USB thumb drive |
| Input Voltage and Frequency Ratings | 100 to 240 VAC, 50/60 Hz |
| Warranty | 2 years |
| Storage Temperature | -10°C to 70°C (14°F to 158°F) |
| Operating Temperature | 4°C to 40°C (40°F to 104°F) |
| Intended Use | For research use only |
| Operating/Storage Humidity | 20% to 80% RH, non-condensing |
| Pollution Degree | IP2X |
| Insulation Category | Category II |
| Regulatory Certifications | CE, ETL (UL, CSA), FCC, WEEE, EU and RoHS |
| Physical Characteristics | |
| Dimensions, W x H x D | 30.5 x 7.8 x 5.4 cm (12 x 6.25 x 14.5 in) |
| Weight | 5.7 kg (12.5 lb) |
| * All data automatically stored in internal memory and may be downloaded to external USB Key. Maximum data logs stored and retrievable from internal memory >20,000. |
|
- Suspension and adherent cell transfection
- Large volume electroporation
- CAR-T cell production
- CRISPR gene editing
- Intradermal, intramuscular and intratumor delivery
- Animal immunization and vaccine research
| Item # | Product |
|---|---|
| 47-0610N | AgilePulse PLUS Safety Stand for Flatpack |
| 47-0620N | AgilePulse PLUS Large Volume Chamber Stand for Large Volume 5 ml Chambers |
| 47-0095 | AgilePulse PLUS HV Output Cable, 5 ft |
| 47-0096 | AgilePulse PLUS HV Output Cable, 10 ft |
| 45-0088 | Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0206 | 2-Needle Array Handle, 5 mm, for 45-0168 |
| 47-0204N | AgilePulse Large Volume Chamber, 6 mm gap, 5 ml, 2 ports w/fittings |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 47-0097 | Adapter, connects AgilePulse PLUS to AgilePulse MAX Safety Stands and Large Volume Chamber Stands |
AgilePulse PLUS Quick Start Guide
REFERENCES
Andreason GL, Evans GA. Optimization of electroporation for transfection of mammalian cells. Anal. Biochem. 1989 Aug 1;180:269-275.
Bartoletti DC, Harrison GI. and Weaver JC. The number of molecules taken up by electroporated cells: quantitative determination. FEBS Lett. 1989:256:4-10.
Buck, JA, Hayt WH. Engineering Electro-Magnetics, 9th Edition. New York:McGraw Hill;2012.
Chang, DC, Chassy BM, Saunders JA and Sowers AE, eds. Guide to Electroporation and Electrofusion. San Diego: Academic Press;1992.
Dimitrov DS, and Sowers AE. Membrane electroporation - fast molecular exchange by electroosmosis. Biochimica et Biophysica Acta. 1990 1022:381-392.
Disalvo EA and Simon SA, eds. Permeability and Stability of Lipid Bilayers. Boca Raton: CRC Press;1995.
Djuzenova CS, Zimmermann U, Frank H, Sukhorukov VL, Richter E, Fuhr G. Effect of medium conductivity and composition on the uptake of propidium iodide into electropermeabilized myeloma cells. Biochimica et Biophysica Acta. 1996;1284:143-152.
Feucht J, et al. Calibration of CAR activation potential directs alternative T cell fates and therapeutic potency. Nature Medicine. 2019;25:82-88.
Golzio M, Teissie J, Rols MP. Direct visualization at the single-cell level of electrically mediated gene delivery. Proc Natl Acad Sci USA. 2002 Feb 5;99(3):1292-7.
Jenkins MJ, Farid SS. Cost-effective bioprocess design for the manufacture of allogeneic CAR-T cell therapies using a decisional tool with multi-attribute decision-making analysis. Biochem Engin J. 2018;137:192-204.
Klenchin VA, Sukharev SM, Chernomordik LV, Chizmadzhev YA, Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis. Biophys J. 1991;60:804-811.
Lee SWL, et al. Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model. Front Immunol. 2018 Mar 6;9:416. doi: 10.3389/fimmu.2018.00416. eCollection 2018.
Markovic SN, et al. Preparing clinical-grade myeloid dendritic cells by electroporation-mediated transfection of in vitro amplified tumor-derived mRNA and safety testing in stage IV malignant melanoma. J Transl. Med. 2006 Aug 15;4:35-48.
McCreedy BJ, Senyukov VV, Nguyen KT. Off the shelf T cell therapies for hematologic malignancies. Best Practice and Research: Clinical Haematology. 2018 Jun 31;2:166-175.
Moyer LS. A suggested standard method for the investigation of electrophoresis. J Bacteriol. 1936 May; 31(5):531-546.
Neumann E, Rosenheck K. Permeability changes induced by electric impulses in vesicular membranes. J Membr. Biol. 1972 Dec 29;10:279-290.
Neuman E, Toensing K, Kakorin S, Budde P, Frey J. Mechanism of electroporative dye uptake by mouse B cells. Biophys J. 1998;74:98-108.
Neuman E, Sowers AE, and Jordan C.A, eds. Electroporation and Electrofusion in Cell Biology. New York: Plenum Press;1989.
Nickoloff, Jac A., ed. Plant Cell Electroporation and Electrofusion Protocols. Methods in MolecularBiology, Volume 55. Totowa, New Jersey:Humana Press;1995.
Rathert P, et al. Transcriptional plasticity promotes primary and acquired resistance to BET inhibition. Nature. 2015 Set24;525(7570):543-547.
Valton J, et al. A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies. Nature Sci Rep. 2018;8: 8972.
