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Details
Get a multifunctional electrofusion and square wave electroporation generator that meets your exact needs. Available product configurations by application, such as embryo manipulation or hybridoma production, make choosing the right system easy.
For the ultimate in performance and convenience the ECM 2001+ offers advanced programming functions, preset protocols, and the most experimental flexibility.
For a reliable, affordable and easy-to-use cell fusion and mammalian transfection system, the ECM 2001 LITE may be just the right choice for you!
ECM 2001 SERIES GENERATORS
ECM 2001+ and ECM 2001 LITE are multifunctional electrofusion and square wave electroporation generators. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. These systems are powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
Waveforms
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.
ECM 2001+ ELECTROFUSION AND ELECTROPORATION SYSTEM
The ECM 2001+ Electrofusion and Electroporation System provides the highest performance and flexibility for cell fusion applications such as hybridoma production, hybrid cell formation, embryo manipulation, nuclear transfer and transgenic applications. Additionally, square wave electroporation functions offer an efficient, reproducible solution for transfecting mammalian cells and tissues with DNA, mRNA, siRNA, proteins, drugs, CRISPR gene editing constructs and nanoparticles.
Features & Benefits
- Separate Electrofusion and Electroporation functional menus for streamlined protocol programming, data organization and navigation
- Advanced User-Defined Electrofusion protocols enable users to independently combine up to 19 AC protocol steps and up 1 DC protocol step in any order, for the highest performance and flexibility
- Convenient Pre-Set Electrofusion protocols for common hybridoma, embryo manipulation, and nuclear transfer applications
- Electrofusion and Electroporation Quick Start menus for speedy access to most used 3-step electrofusion and most used 1-step electroporation parameters
- Storage of up to 1,000 advanced User-Defined protocols
ECM 2001 LITE ELECTROFUSION AND ELECTROPORATION SYSTEM
The ECM 2001 LITE Electrofusion and Electroporation System offers a simple, effective option for cell fusion and square wave electroporation. The Quick Start Electrofusion menu offers a preconfigured 3-step protocol consisting of AC alignment, DC square wave, and AC.
Features & Benefits
- Quick Start Electrofusion menu with 3-step AC-DC-AC protocol structure for speedy access to the most used experimental parameters
- Storage of up to 1,000 User-Defined protocols with 3-step AC-DC-AC protocol structure
- Flexibility to inactivate the Electrofusion protocol AC steps for use in square wave electroporation applications, or to inactivate the Electrofusion protocol DC step for embryo manipulation applications
APPLICATIONS
Electrofusion
Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC sine wave and DC square wave pulses. Fusion is achieved by the generation of an AC current sine waveform that generates a benign dielectrophoretic alignment of cells. Next, a DC square waveform is applied to promote fusion between aligned cells. After fusion, the AC sine waveform is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.
Mammalian Cell Transfection
Square wave electroporation is a highly flexible technique used to modify mammalian cells. Whether you are regulating gene expression up or down, expressing recombinant proteins, delivering non-cell-permeant molecules, or creating genetically modified model systems, electroporation is an ideal delivery method for all types of nucleic acids, proteins, drugs, and nanoparticles.
This method is non-toxic and requires no expensive reagents to successfully transfect your cells. Alternatively, adding additional selection steps to isolate stably transfected cells allows for integration of a gene into the genome of the cell for long term expression of protein.
The use of ECM 2001 series generators offers the control needed to adjust electrical settings for optimization of parameters. These systems are powerful enough to yield high transfection efficiencies and great survival rates for cell lines and difficult to transfect cell types including stem cells and primary cells.
In Vivo, In Utero & In Ovo Transfection
Square wave systems allow researchers to set the pulse lengths and number of pulses, which is critical to ensure viable cells and tissues while still maintaining efficient transfection both in vivo and ex vivo. Electroporation-mediated gene and drug delivery has been shown to substantially increase intracellular uptake and expression of DNA, siRNA and miRNA in muscle, skin, liver, kidney, testis, retina, tumors, etc. In vivo electroporation has successfully been used in embryo applications, in utero and in ovo applications, and in mammals, as well as other classical model systems such as chicken eggs, insects, and zebra fish.
PRODUCT CONFIGURATIONS BY APPLICATION
Complete Cell Fusion and Electroporation System
For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001 Cell Fusion Systems (45-2045, 45-2065) are our most versatile instrument configurations. The ECM 2001 Cell Fusion System comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.
Mammalian Cell Transfections
For mammalian cell electroporation, ECM 2001 Electroporation Systems (45-2046, 45-2066) come with a safety stand and cuvettes for small volume in vitro electroporation. ECM 2001 Electroporation Systems with Monitoring (45-2050, 45-2070) additionally include a high voltage probe, connection box and oscilloscope for precise wave form monitoring and quality control. Combine Electroporation Systems with electrodes and accessories available separately for large volume in vitro electroporation, in vivo electroporation, adherent electroporation, or cell fusion applications.
Embryo Manipulation
For use in small volume cell fusion applications such as embryo manipulation, we recommend ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In these systems the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.
Hybridoma Production
For use in large volume cell fusion applications such as hybridoma production, ECM 2001 generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. These systems (45-2048, 45-2068) do not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.
Embryo Manipulation
For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.
Adherent Cell Transfections
Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001 series generators coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.
Build Your Own Custom System
Combine ECM 2001 Generators (45-2049, 45-2069) with separately available BTX electrofusion and electroporation electrodes and accessories to tailor the system to your experimental needs.
INCLUDED ITEMS
| Item # | Description | Included Items |
|---|---|---|
| 45-2045 | ECM 2001+ Cell Fusion System | ECM 2001+ Generator, Microslides (0.5 mm gap, 3.2 mm gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-2046 | ECM 2001+ Electroporation System, for Cuvettes | ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-2047 | ECM 2001+ Embryo Manipulation System | ECM 2001 Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables |
| 45-2048 | ECM 2001+ Hybridoma System | ECM 2001+ Generator, 2 ml Coaxial Optimization Chamber (470030), 9 ml Coaxial Production Chamber, Female/Female Adapter Set, Cytofusion Medium C (500 ml) |
| 45-2049 | ECM 2001+ Generator Only | ECM 2001+ Generator, 5 ft High Voltage Output Cable, F/F adapter set |
| 45-2065 | ECM 2001 LITE Cell Fusion System | ECM 2001 LITE Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-0266 | ECM 2001 LITE Electroporation System | ECM 2001 LITE Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack |
| 45-2067 | ECM 2001 LITE Embryo Manipulation System | ECM 2001 LITE Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber |
| 45-2068 | ECM 2001 LITE Hybridoma Production System | ECM 2001 LITE Generator, 2 ml Coaxial Optimization Chamber, 9 ml Coaxial Production Chamber, Female/Female Adapter Set, BTX Cytofusion Medium C 500 ml |
| 45-2069 | ECM 2001 LITE Generator Only | ECM 2001 LITE Generator, 5 ft High Voltage Output Cable, F/F adapter setl |
This instrument is intended for Research and Pre-Clinical use only and is not for use in diagnostic or clinical procedures.
| ECM 2001+ | ECM 2001 LITE | |
| Firmware Specifications | ||
| Electrofusion Quick Start Menu | Up to 3 protocol steps, ordered AC-DC-AC | Up to 3 protocol steps, ordered AC-DC-AC |
| Electrofusion User Defined Protocols | Up to 19 AC protocol steps and up to 1 DC step, organized in any order | Up to 3 protocol steps, ordered AC-DC-AC |
| Electrofusion Preset Protocols | Over 20 Preset Protocols for electrofusion applications | Not avialable |
| Electroporation Quick State Menu | Yes | Not available |
| Electroporation User Defined Protocols | Yes | Not available |
| Square Wave Pulse, DC | ||
| Voltage Range | ||
| LV Mode | 5 to 500 V in 1 V steps | 5 to 500 V in 1 V steps |
| HV Mode | 505 to 3000 V in 1 V steps | 505 to 3000 V in 1 V steps |
| Voltage Accuracy | ||
| LV Mode | 5% | 5% |
| HV Mode | 5% | 5% |
| Pulse Length Range | ||
| LV Mode | 10 to 999 µs in 1 µs steps | 10 to 999 µs in 1 µs steps |
| LV Mode | 1 to 999 ms in 1 ms steps | 1 to 999 ms in 1 ms steps |
| HV Mode | 10 to 600 µs in 1 µs steps | 10 to 600 µs in 1 µs steps |
| Multiple Pulsing | ||
| LV Mode | 0 to 100 pulses per sample | 0 to 100 pulses per sample |
| HV Mode | 0 to 100 pulses per sample | 0 to 100 pulses per sample |
| Pulse Interval | 0.1 s to 10 s | 0.1 s to 10 s |
| Alignment (Pre-Pulse), AC | ||
| Frequency | 0.2 to 2 MHz in 0.1 MHz steps | 0.2 to 2 MHz in 0.1 MHz steps |
| Voltage | 5 to 75 V in 5 V steps | 5 to 75 V in 5 V steps |
| Duration | 0 to 99 s in 1 s steps | 0 to 99 s in 1 s steps |
| Wave Shape | Sine wave | Sine wave |
| Post-Fusion, AC | ||
| Frequency | 0.2 to 2 MHz in 0.1 MHz steps | 0.2 to 2 MHz in 0.1 MHz steps |
| Voltage | 5 to 75 V in 5 V steps | 5 to 75 V in 5 V steps |
| Duration | 5 to 75 V in 5 V steps | 5 to 75 V in 5 V steps |
| Wave Shape | Sine wave | Sine wave |
| Sample Load Ranges | ||
| AC | ||
| All Voltages | Load must be ≥60 Ω> | Load must be ≥60 Ω> |
| DC | ||
| LV Mode | Pulse Length <100 ms, Load must be >8 to 9 Ω, Pulse Length >100 ms, Load must be >100 Ω |
Pulse Length <100 ms, Load must be >8 to 9 Ω, Pulse Length >100 ms, Load must be >100 Ω |
| HV Mode | Pulse Length ≤600 ms Load must be ≥40 Ω |
Pulse Length ≤600 ms Load must be ≥40 Ω |
| Other Specifications | ||
| Charging Time | LV = 7 s, HV = 7 s | LV = 7 s, HV = 7 s |
| DC Monitoring | Protocol Name, Steps Executed, Time/Date, Error Conditions, Pulse Voltage, Pulse Current, Pulse Width, Pulse #, Droop %, Sample Voltage, Sample Current and Sample Resistance |
Protocol Name, Steps Executed, Time/Date, Error Conditions, Pulse Voltage, Pulse Current, Pulse Width, Pulse #, Droop %, Sample Voltage, Sample Current and Sample Resistance |
| Display | 7-inch full color display | 7-inch full color display |
| Controls | Touch Screen | Touch Screen |
| Programmability | Stores over 1000 protocols | Stores over 1000 protocols |
| DC Arc Control | Yes | Yes |
| Pre-Pulse Sample Load Check | Ves (20 VDC) | Ves (20 VDC) |
| Computer (Optional) | PC running Microsoft Windows XP, 7, 8.1 or 10 | PC running Microsoft Windows XP, 7, 8.1 or 10 |
| Communications | USB | USB |
| Data File Format | Comma Separated Values (CSV) | Comma Separated Values (CSV) |
| Remote Operation | Yes, using Foot Pedal control | Yes, using Foot Pedal control |
| Accessory Connector Type | Coaxial | Coaxial |
| Power Ratings | 70 W idle and 900 W pulsing | 70 W idle and 900 W pulsing |
| Input Voltage Ratings | 100 to 240 VAC, 50/60 Hz | 100 to 240 VAC, 50/60 Hz |
| Dimensions (H x W x D) | 13 x 12 x 13 in | 13 x 12 x 13 in |
| Weight | 22 lb | 22 lb |
| Warranty | 2 years | 2 years |
| Atmospheric Specifications | ||
| Operating Temperature | 4°C to 40°C (40°F to 104°F) | 4°C to 40°C (40°F to 104°F) |
| Storage Temperature | -10°C to 70°C (14°F to 158°F) | -10°C to 70°C (14°F to 158°F) |
| Operating Humidity | 20% to 80% RH, non-condensing | 20% to 80% RH, non-condensing |
| Storage Humidity | 20% to 80% RH, non-condensing | 20% to 80% RH, non-condensing |
| Mode of Operation | Continuous | Continuous |
| Classification | Class I | Class I |
| Pollution | IP2X | IP2X |
| Installation | Category II | Category II |
| Regulatory Certifications | CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme | CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme |
- Cell fusion
- Hybridoma production
- Nuclear transfer
- Embryo manipulation
- Mammalian cell transfection
- Plant protoplast fusion
- Stem cell production
| Item # | Product |
|---|---|
| 45-0110 | Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50 |
| 47-0206 | Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10 |
| 45-0109 | Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50 |
| 45-0167 | 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle |
| 45-0168 | 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle |
| 45-0531 | Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable |
| 45-0530 | Adherent Cell Electrode, 5 mm gap |
| 47-0001 | BTXpress Cytofusion Medium C, 500 ml volume |
| 47-0003 | BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume |
| 47-0020 | 9 ml Coaxial Chamber for production |
| 47-0030 | Hybrimune 2 ml Coaxial Chamber for optimization |
| 45-0134 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray |
| 45-0124 | Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray |
| 45-0135 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue |
| 45-0125 | Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue |
| 45-0136 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow |
| 45-0126 | Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow |
| 45-0208 | Cuvette Rack, 20 numbered positions |
| 45-0087 | ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables |
| 45-0217 | ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode) |
| 45-0086 | Legacy ECM 2001 CE Remote Control with 45-0085 cable |
| 45-0088 | Female/Female Adapter Set, for 4 mm diameter Banana Plug Cables |
| 45-0214 | ECM 2001 Footswitch (for non-CE 2001 only) |
| 45-0216 | ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish) |
| 47-0208 | ECM 2001 Safety Stand for Flatpack |
| 45-0108 | Flat Electrode |
| 45-0161 | Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0162 | Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0160 | Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables |
| 45-0117 | Genetrodes, 1 mm L-Shape (GOLD TIP) |
| 45-0164 | Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0114 | Genetrodes, 10 mm Straight (GOLD TIP) |
| 45-0116 | Genetrodes, 3 mm L-Shape (GOLD TIP) |
| 45-0163 | Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables |
| 45-0115 | Genetrodes, 5 mm L-Shape (GOLD TIP) |
| 45-0113 | Genetrodes, 5 mm Straight (GOLD TIP) |
| 45-0106 | Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1 |
| 45-0105 | Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1 |
| 45-0103 | Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10 |
| 45-0104 | Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10 |
| 45-0100 | Petri Dish Electrode |
| 45-0506 | Petri Dish Platinum Electrode Chamber, 15 mm gap |
| 45-0504 | Petri Dish Platinum Electrode Chamber, 5 mm gap |
| 45-0507 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap |
| 45-0505 | Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap |
| 45-0130 | Petri Pulser |
| 45-0486 | Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables) |
| 45-0524 | Platinum Tweezertrode, 1 mm Flat |
| 45-0487 | Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable) |
| 45-0489 | Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables) |
| 45-0488 | Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables) |
| 45-0207 | Safety Stand, Adjustable Gap (for use with Cuvettes) |
| 45-0119 | Stainless Tweezertrode Electrode, 10 mm Diameter |
| 45-0118 | Stainless Tweezertrode Electrode, 7 mm Diameter |
| 45-0166 | Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable) |
| 45-0165 | Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable) |
| 45-0493 | Triple Electrode Tweezertrode, 3 mm |
| 45-0494 | Triple Electrode Tweezertrode, 5 mm |
Cell Fusion Applications: Electrofusion vs. PEG
REFERENCES
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