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Petri Dish Platinum Electrodes and Chambers for Tissue Slices

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Petri Dish Platinum Electrodes and Chambers for Tissue Slices
Electrodes and chambers for delicate and/or difficult tissue transfection
Product Item # Price  
Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 10 mm 45-0500   View Price
Petri Dish Platinum Electrode for Tissue Slices Chamber Kit, 7 mm 45-0490   View Price
Petri Dish Platinum Electrode Chamber, 10 mm, negative 45-0501   View Price
Petri Dish Platinum Electrode Chamber, 7 mm, negative 45-0491   View Price
Platinum Electrode Wand, 10 mm, positive 45-0502   View Price
Platinum Electrode Wand, 7 mm, positive 45-0492   View Price
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Details

The Petri dish platiunum electrodes are designed for delicate and/or difficult ex vivo tissue transfection. Ex vivo electroporation is an efficient, effective method to introduce genes, drugs or any number of molecules into ex-plant tissues. A common application is mouse brain slice for studying neuronal development. This specialty electrode makes transfection quick and simple and is compatible with the BTX ECM 830, ECM 2001+, Gemini X2 and Legacy ECM 2001 generators. The electrode is comprised of two parts, the glass petri dish electrode and electrode wand. The glass petri dish contains a square shaped platinum electrode chamber to secure the tissue. The wand incorporates an identical shaped platinum electrode, which is placed over the chamber to complete electroporation. This sandwich configuration ensures a homogeneous field of energy for optimum transfection.

REQUIRED CABLES

Required for connection to Gemini X2 and ECM 830
45-0503 Micrograbber Cable for Tissue Slice Chamber, Negative
45-0204 Adapter Banana Plug Cables, Red and Black
Required for connection to ECM 2001+
45-0503 Micrograbber Cable for Tissue Slice Chamber, Negative
45-0204 Adapter Banana Plug Cables, Red and Black
45-0088 Female/Female Adapter Set for Banana Plug Cables
45-2057 or 45-2058 ECM 2001+ HV Output Cable, 5 ft or 10 ft length
Required for connection to Legacy ECM 2001
45-0503 Micrograbber Cable for Tissue Slice Chamber, Negative
45-0204 Adapter Banana Plug Cables, Red and Black
45-0088 Female/Female Adapter Set for Banana Plug Cables
45-0083 Coaxial to Banana Plug Cables, Red and Black, 10 ft

 

USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS

In this example, the Gemini X2 generator is connected to the electrodes using the sequence of cables and adapters illustrated below.

+ +
 Gemini X2 System    45-0204 Adapter Cables  

45-0503 Single Adapter Cable for Wand

+ selected Petri Dish Electrode

 

USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM

In this example, the ECM 2001+ generator is connected to the electrodes using the sequence of cables and adapters illustrated below.

+ + + +
 ECM 2001+ System    45-2057 or 45-2058 HV Output Cable    45-0088 Female/Female Adapters    45-0204 Adapter Cables   45-0503 Single Adapter Cable for Wand + selected Petri Dish Electrode

 

USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM

In this example, the Legacy ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below.

+ + + +
 Legacy ECM 2001 System    45-0083 Coaxial Cables    45-0088 Female/Female Adapters    45-0204 Adapter Cables   45-0503 Single Adapter Cable for Wand + selected Petri Dish Electrode

Included Items

Item # Name Included Items
45-0500 Tissue Slice Platinum Chamber Electrode Kit, 10 mm Petri Dish Platinum Electrode Chamber, 10 mm and a Platinum Wand Electrode, 10mm, with micrograbber cable and single adaptor cable
45-0490 Tissue Slice Platinum Chamber Electrode Kit, 7 mm Petri Dish Platinum Electrode Chamber, 7 mm and a Platinum Wand Electrode,7 mm, with micrograbber cable and single adaptor cable
45-0491 Petri Dish Platinum Tissue Slice Electrode Chamber Chamber only, 7 mm Requires Platinum Electrode Wand either 45-0492 or 45-0502 and cables 45-0503 and 45-0511
45-0501 Petri Dish Platinum Tissue Slice Electrode Chamber Chamber only, 10 mm Requires Platinum Electrode Wand either 45-0492 or 45-0502 and cables 45-0503 and 45-0511
45-0492 Platinum Electrode Wand Wand only, 7 mm Requires Platinum Tissue Slice Chamber either 45-0491 or 45-0501 and cables 45-0503 and 45-0511
45-0502 Platinum Electrode Wand Wand only, 10 mm Requires Platinum Tissue Slice Chamber either 45-0491 or 45-0501 and cables 45-0503 and 45-0511
Generator Compatibility ECM 830, ECM 2001+, Legacy ECM 2001, and Gemini X2
Voltage Range 0 to 100 Volts
Pulse Length 10 μs to 100 ms
Chamber Depth 1 to 99 mm (depending on voltage)
Electrode Material Platinum
Wand Material Platinum
Dimensions: 
  Dish Electrode 7 mm (L x W x D)  7 mm x 7 mm x 1 mm
  Wand Electrode 7 mm (L x W x D)  7 mm x  7 mm
  Dish Electrode 10 mm (L x W x D) 10 mm x 10 mm x 1 mm
  Wand Electrode 10 mm (L x W x D) 10 mm x 10 mm
  • Ex vivo electroporation
Item # Product
45-0083 ECM 2001 Coaxial to Banana Plug Cables, Red and Black, 10 ft
45-0088 ECM 2001 Female/Female Adapter Set for Banana Plug Cables (Use with 45-0216)
45-0511 Single Adapter Cable for Wand Electrode, Positive (for use with Tissue Slice Electrodes)