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ECM 2001+ Electrofusion & Electroporation System


ECM 2001+ Electrofusion & Electroporation System

NEW MODEL WITH UPDATED FUNCTIONALITY!


Powerful multi-purpose system providing fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines


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Product Item # Price  
ECM 2001+ Cell Fusion System, Complete 45-2045   View Price
ECM 2001+ Hybridoma Production System, with Hybrimune 2 ml Coaxial Optimization Chamber 98-6169   View Price
ECM 2001+ Electroporation System Complete, for Cuvettes 45-2046   View Price
ECM 2001+ Embryo Manipulation System 45-2047   View Price
ECM 2001+ Generator Only 45-2049   View Price
ECM 2001+ Electroporation System Complete, for Cuvettes with Monitoring 45-2050   View Price
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Details

The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.

ECM 2001 GENERATOR

The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.

WAVEFORMS

AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.

FEATURES & BENEFITS

  • AC waveform of 0.2 – 2.0 MHz
  • Square wave electroporation capabilities
  • A wide range of voltages from 5 V to 3000 V
  • Fine voltage discrimination
  • Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
  • Capable of operating at low impedance loads
  • Large touchscreen interface

APPLICATIONS

ELECTROFUSION

Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC sine wave and DC square wave pulses. Fusion is achieved by the generation of an AC current sine waveform that generates a benign dielectrophoretic alignment of cells. Next, a DC square waveform is applied to promote fusion between aligned cells. After fusion, the AC sine waveform is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.

ELECTROPORATION

Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used in transient transfections to study protein expression or to temporarily knockout or “silence” these genes using siRNA. This is used to study gene targeting and function.

Square waveform electroporation is an efficient non-viral method for transfecting genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and to understand protein regulation.

Alternatively, adding additional selection steps to isolate stably transfected cells allows for ;integration of a gene into the genome of the cell for long term expression of protein. The use of the ECM 2001+ offers the control needed to adjust electrical settings for optimization of parameters. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.


PRODUCT CONFIGURATIONS BY APPLICATION

Complete Cell Fusion and Electroporation System

For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001+ Cell Fusion System complete (45-2045) is our most versatile instrument configuration. The ECM 2001+ Cell Fusion system complete comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.

Hybridoma Production

For use in large volume cell fusion applications such as hybridoma production, the ECM 2001+ generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. This system (98-6169) does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001+ Embryo Manipulation System (45-2047). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Mammalian Cell Transfections

Utilizing the ECM 2001+ System Complete, for Cuvettes (45-2046) for standard mammalian cell transfections is simple. The AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 10 pulses per experiment, make this an extremely versatile system for any lab.

Adherent Cell Transfections

Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001+ coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.


INCLUDED ITEMS

Item #DescriptionIncluded Items
45-2045 ECM 2001+ Cell Fusion System Complete ECM 2001+ Generator, Microslides (0.5 mm gap, 3.2 mm gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack
45-2046 ECM 2001+ Electroporation System Complete, for Cuvettes ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 
45-2047 ECM 2001+ Embryo Manipulation System ECM 2001 Generator, Microslides, round wire (0.5 mm gap, 1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-2049 ECM 2001+ Generator Only ECM 2001+ Generator, 5 ft High Voltage Output Cable, F/F adapter set
45-2050 ECM 2001+ System Complete, for Cuvettes with Monitoring  ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), Cuvette Rack, Enhancer 3000 Probe, Enhancer Interface Box, Oscilloscope and Cables
98-6169 ECM 2001+ Hybridoma System, with Hybrimune 2 ml Coaxial Optimization Chamber  ECM 2001+ Generator, 2 ml Coaxial Optimization Chamber (470030), 9 ml Coaxial Production Chamber, Female/Female Adapter Set, Cytofusion Medium C (500 ml)

 

Square Wave Pulse, DC
Voltage Range 
   LV Mode  5 to 500 V in 1 V steps
   HV Mode  505 to 3000 V in 1 V steps
 Voltage Accuracy 
   LV Mode  5%
   HV Mode  5%
 Pulse Length Range 
   LV Mode  10 to 999 µs in 1 µs steps
   LV Mode  1 to 999 ms in 1 ms steps
   HV Mode  10 to 600 µs in 1 µs steps
 Multiple Pulsing 
   LV Mode  1 to 10 pulses per sample
   HV Mode  1 to 10 pulses per sample
 Pulse Interval  0.1 s to 10 s
 Alignment (Pre-Pulse), AC 
 Frequency  0.2 to 2 MHz in 0.1 MHz steps
 Voltage   5 to 75 V in 5 V steps
 Duration   0 to 99 s in 1 s steps
 Wave Shape   Sine wave
 Post-Fusion, AC 
 Frequency   0.2 to 2 MHz in 0.1 MHz steps
 Voltage   5 to 75 V in 5 V steps
 Duration   0 to 99 s in 1 s steps
 Wave Shape   Sine wave
 Sample Load Ranges 
 AC  
   All Voltages   Load must be ≥60 Ω>
 DC  
   LV Mode  Pulse Length <100 ms,
 Load must be >8 to 9 Ω,
 Pulse Length >100 ms,
 Load must be >100 Ω
   HV Mode  Pulse Length ≤600 ms
 Load must be ≥40 Ω
 Other Specifications 
 Charging Time  LV = 7 s, HV = 7 s
 DC Monitoring  Protocol Name, Steps Executed,
 Time/Date, Error Conditions, Pulse
 Voltage, Pulse Current,
 Pulse Width, Pulse #, Droop %,
 Sample Voltage, Sample Current and
 Sample Resistance
 Display  7-inch full color display
 Controls  Touch Screen
 Programmability  Stores over 1000 protocols
 DC Arc Control  Yes
 Pre-Pulse Sample Load Check  Ves (20 V DC)
 Computer (Optional)  PC running Microsoft Windows XP, 7, 8.1 or 10
 Communications  USB
 Data File Format  Comma Separated Values (CSV)
 Remote Operation  Yes, using Foot Pedal control
 Accessory Connector Type  Coaxial
 Power Ratings  70 W idle and 900 W pulsing
 Input Voltage Ratings  100 to 240 VAC, 50 / 60 Hz
 Dimensions (H x W x D)  13 x 12 x 13 in
 Weight  22 lb
 Warranty  2 years
 Atmospheric Specifications 
   Operating Temperature   4°C to 40°C (40°F to 104°F)
   Storage Temperature   -10°C to 70°C (14°F to 158°F)
   Operating Humidity  20% to 80% RH, non-condensing
   Storage Humidity   20% to 80% RH, non-condensing
 Mode of Operation  Continuous
 Classification  Class I
 Pollution  IP2X
 Installation  Category II
 Regulatory Certifications  CE, ETL (UL, CSA), FCC, WEEE, EU RoHS & CB Scheme

 

 

  • Cell fusion
  • Hybridoma production
  • Nuclear transfer
  • Embryo manipulation
  • Mammalian cell transfection
  • Plant protoplast fusion
  • Stem cell production
Item # Product
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10
47-0001 BTXpress Cytofusion Medium C, 500 ml volume
47-0003 BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume
47-0020 9 ml Coaxial Chamber for production
47-0030 Hybrimune 2 ml Coaxial Chamber for optimization
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0087 ECM 2001 Adapter Set, Micrograbber to Banana Plug Cables
45-0217 ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)
45-0086 Legacy ECM 2001 CE Footswitch with 45-0085 cable
45-0088 ECM 2001 Female/Female Adapter Set for Banana Plug Cables (Use with 45-0216)
45-0214 ECM 2001 Footswitch (for non-CE 2001 only)
45-0085 ECM 2001 Footswitch Cable
45-0216 ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)
47-0208 ECM 2001 Safety Stand for Flatpack
45-0059 Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America)
45-0108 Flat Electrode
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0100 Petri Dish Electrode
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0130 Petri Pulser
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

ECM 2001+ Data Sheet

 

ECM 2001+ User's Manual

 

Cell Fusion Applications: Electrofusion vs. PEG

 

Declaration of Conformity

 

REFERENCES

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Xie Z, et al. Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts. Sci Rep. 2017 Jun;7: 3036. doi: 10.1038/s41598-017-02785-y.

 

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Ruiz N, et al. Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation. PLoS One. 2015 Jun; 10(6): e0130008.

 

Wang, K, et al. Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Sci Rep. 2015 Nov;13(5): 16623.

 

Wang Y, et al. MiR-499 Enhances the Cisplatin Sensitivity of Esophageal Carcinoma Cell Lines by Targeting DNA Polymerase β. Cell Physiol Biochem. 2015;36: 1587-1596.

 

Wu H, et al. TALE Nickase-Mediated SP110 Knockin Endows Cattle with Increased Resistance to Tuberculosis. Proc Natl Acad Sci USA. 2015 Mar;112(13): E1530-1539.

 

Zhang XH, Lian XD, Dai ZX, Zheng HY, Chen X, Zheng YT. α3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2015 Sep;199(6) :2030-2042.

 

Lai CW, et al. FTSJ2, a Heat Shock-Inducible Mitochondrial Protein, Suppresses Cell Invasion and Migration. PLoS One. 2014 Mar; 9(3): e90818.

 

Zhang Y, Luo W, Wang Y, Liu Y, and Zheng, L. Purified dendritic cell-tumor fusion hybrids supplemented with non-adherent dendritic cells fraction are superior activators of antitumor immunity. PLoS One, 2014;9(1), e86772.

 

Liu X, et al. Zinc-Finger Nickase-Mediated Insertion of the Lysostaphin Gene into the Beta-Casein Locus in Cloned Cows. Nat Comm. 2013;4: 2565.

 

Tan, C, et al. Impact of anti-CD25 monoclonal antibody on dendritic cell-tumor fusion vaccine efficacy in a murine melanoma model. J Transl Med. 2013 Jun;11: 148.

 

Wang Y, et al. Mir-655 Up-Regulation Suppresses Cell Invasion by Targeting Pituitary Tumor-Transforming Gene-1 in Esophageal Squamous Cell Carcinoma. J Transl Med. 2013;11: 301. 

 

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