Unleash the potential of CRISPR with BTX Electroporation Systems

Your current CRISPR technology may be limiting the potential of your CRISPR applications. Electroporation has become the method of choice for CRISPR gene editing applications, quickly overtaking traditional methods such as chemical reagents or microinjection.

BTX has been in the forefront of electroporation technology since introducing the first commercial electroporator in 1983. Our latest electroporation instruments are leading the charge for CRISPR gene editing applications! BTX Gemini and ECM 830 Electroporation Systems provide efficient, reproducible delivery of CRISPR/Cas 9, guide RNA, and homologous repair donor constructs to virtually any cell or tissue of interest.

Gemini Twin Wave Universal Electroporation System

These flexible twin wave systems allow both square wave and exponential decay wave electroporation in a single unit. Ideal for CRISPR and many other electroporation applications including, in vivo, in vitro, in ovo and more.

Software includes preset CRISPR protocols for mammalian cells and tissues, bacteria, and other microorganisms.
Choose Gemini SC for cuvette-based in vitro transfections of any cell type in suspension.
With Gemini X2, transfect any cell or tissue using cuvettes, high throughput electroporation plates, and in vivo specialty electrode formats.

Learn more about Gemini Systems

ECM 830 Mammalian Transfection System

This versatile square wave electroporation system is designed for gene, drug and protein delivery in mammalian cells and tissues, including CRISPR applications.

Software includes preset CRISPR protocols for mammalian cells and tissues.
Transfect mammalian cells or tissues using cuvettes, high throughput electroporation plates, and in vivo specialty electrode formats.
Well established publication record with CRISPR gene editing technologies.

Learn more about the ECM 830.

	Contact Us to request a quote or a free demo.

	Interested in learning more about BTX electroporation and CRISPR? Download our Leading the CRISPR Charge brochure for more about electroporation for CRISPR applications, selection guides, and ordering information.

	Download our latest Application Note CRISPR-Mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-Based Gene Transfer.

Selected References

Bhowmik, P., et al. Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Scientific reports. 2018; 8.

Feng, W., et al. CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. J. Vis. Exp. 2018;136. doi: 10.3791/57311.

Kieper, SN, et al. Cas4 facilitates PAM-compatible spacer selection during CRISPR adaptation. Cell reports. 2018; 22(13):3377-3384.

Li, P., et al. Allele-specific CRISPR-Cas9 genome editing of the single-base P23H mutation for rhodopsin-associated dominant retinitis pigmentosa. The CRISPR Journal. 2018;1(1): 55-64.

Long, S, et al. CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii. Bio-protocol. 2018;8(6). pii: e2768. doi: 10.21769/BioProtoc.2768.

Sidik, SM, et al. CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii. Nature protocols. 2018; 13(1):307.

Callif, BL, et al. The application of CRISPR technology to high content screening in primary neurons. Molecular and Cellular Neuroscience. 2017;80:170-179.

Liao, HK, et al. In vivo target gene activation via CRISPR/Cas9-mediated trans-epigenetic modulation. Cell. 2017; 171(7):1495-1507.

Yao X, et al. CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms. EBioMedicine. 2017;20:19-26.

Qin W, et al. Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. Genetics. 2015;200:423-430.

Sidik S, et al. Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9. PLoS ONE. 2014;9: e100450.

Wang X, et al. Efficient CRISPR/Cas9-Mediated Biallelic Gene Disruption and Site-Specific Knockin after Rapid Selection of Highly Active sgRNAs in Pigs. Sci Rep. 2015;5:13348.

Xie Z, et al. Optimization of a CRISPR/Cas9-Mediated Knock-In Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts. Sci Rep. 2017;7:3036.

Dimitrov L, et al. Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells. PLoS ONE. 2016;11: e0154303.

Shin SE, et al. CRISPR/Cas9-Induced Knockout and Knock-in Mutations in Chlamydomonas reinhardtii. Sci Rep. 2016 6:27810.

An L, et al. Efficient Generation of FVII Gene Knockout Mice using CRISPR/Cas9 Nuclease and Truncated Guided RNAs. Sci Rep. 2016;6:25199.