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Meander Fusion Chamber, pkg 4

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Meander Fusion Chamber, pkg 4
Novel microslide designed specifically used for electro cell fusion. Allows direct viewing of dimer formation during alignment.
Product Item # Price  
Meander Fusion Chamber, pkg 4 45-0107   View Price
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Details

The BTX Meander Fusion Chamber is a novel microslide design which is specifically used for electro cell fusion. The Meander Fusion Chamber generates a divergent field and is used for fusion of mammalian cells, plant, yeast, fungi and microorganisms.

This specialty electrode is constructed of a conductive metal alloy. It has two primary bars that are connected by many tiny fingerlike projections. These projections are spaced 0.2 mm apart. This electrode is mounted on a glass slide. It is designed for direct viewing of dimer formation during alignment while under a microscope.

The Meander Fusion Chamber is compatible with the ECM 2001+, Legacy ECM 2001, ECM 830, or Gemini X2 pulse generators. Cao et al. 1995 used the Meander Fusion Chamber to generate quadromas secreting bifunctional antibodies. He found this method both rapid and economical to produce several quadromas in a short time.

REQUIRED CABLES

Required for connection to ECM 2001+
45-0087 Micrograbber Adapter Set, Red and Black, for Banana Plug Cables
45-2057 or 45-2058 ECM 2001+ HV Output Cable, 5 ft or 10 ft length
Required for connection to Gemini X2 and ECM 830
45-0216 Micrograbber to Banana Plug Connection Cables, 10 ft
Required for connection to Legacy ECM 2001
45-0216 Micrograbber to Banana Plug Connection Cables, 10 ft
45-0088 Female/Female Adapter Set for Banana Plug Cables
45-0083 Coaxial to Banana Plug Cables, Red and Black, 10 ft

 

USE OF MEANDER FUSION CHAMBER WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM

In this example, the ECM 2001+ generator is connected using the sequence of cables and adapters illustrated below, then connected to the Meander Fusion Chamber. 

+ +
 ECM 2001+ System  

 45-2057 or 45-2058 HV Output Cable

   45-0087 Micrograbber Adapters 
Meander Fusion Chamber

 

USE OF MEANDER FUSION CHAMBER WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS

In this example, the Gemini X2 generator is connected using the sequence of cables and adapters illustrated below, then connected to the Meander Fusion Chamber. 

+  
 Gemini X2 System    45-0216 Micrograbber Cables 
Meander Fusion Chamber
 

 

USE OF THE MEANDER FUSION CHAMBER WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM

In this example, the Legacy ECM 2001 generator is connected to the chamber using the sequence of cables and adapters illustrated below.

+ + +  
Legacy ECM 2001 System   45-0083 Coaxial Cables   45-0088 Female/Female Adapters    45-0216 Micrograbber Cables 
Meander Fusion Chamber
Generator Compatibility ECM 2001+, Legacy ECM 2001, ECM 830, and Gemini X2
Field Type Divergent
Max Voltage:  
AC  16 V (0 - peak) 
DC  0 to 480 V 
Pulse Length  1 µsec to 99 msec
Number of Pulses  1 to 99 (depending on voltage)
Gap Size  0.2 mm 
Autoclavable  No 
  • Cell Fusion
  • Plant Protoplast Fusion
Item # Product
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1

Meander Fusion Chamber User's Manual

 

REFERENCES

Cao et al. A rapid non-selective method to generate quadromas by microelectrofusionJournal of Immunological Methods. 187: 1-7 (1995).

 

Von Keller et al. Intracelluar movements and reorganization of electrically fused sunflower protoplasts. Int. J. Plant Sci. 156(6): 764-773 (1995).