800-272-2775
Details
The Petri dish platiunum electrodes are designed for delicate and/or difficult ex vivo tissue transfection. Ex vivo electroporation is an efficient, effective method to introduce genes, drugs or any number of molecules into ex-plant tissues. A common application is mouse brain slice for studying neuronal development. This specialty electrode makes transfection quick and simple and is compatible with the BTX ECM 830, ECM 2001+, Gemini X2 and Legacy ECM 2001 generators. The electrode is comprised of two parts, the glass petri dish electrode and electrode wand. The glass petri dish contains a square shaped platinum electrode chamber to secure the tissue. The wand incorporates an identical shaped platinum electrode, which is placed over the chamber to complete electroporation. This sandwich configuration ensures a homogeneous field of energy for optimum transfection.
REQUIRED CABLES
| Required for connection to Gemini X2 and ECM 830 | |
| 45-0503 | Micrograbber Cable for Tissue Slice Chamber, Negative |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| Required for connection to ECM 2001+ | |
| 45-0503 | Micrograbber Cable for Tissue Slice Chamber, Negative |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| 45-0088 | Female/Female Adapter Set for Banana Plug Cables |
| 45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
| Required for connection to Legacy ECM 2001 | |
| 45-0503 | Micrograbber Cable for Tissue Slice Chamber, Negative |
| 45-0204 | Adapter Banana Plug Cables, Red and Black |
| 45-0088 | Female/Female Adapter Set for Banana Plug Cables |
| 45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS
In this example, the Gemini X2 generator is connected to the electrodes using the sequence of cables and adapters illustrated below.
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+ |  |
+ |  |
| Gemini X2 System | 45-0204 Adapter Cables |
45-0503 Single Adapter Cable for Wand + selected Petri Dish Electrode |
USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001+ generator is connected to the electrodes using the sequence of cables and adapters illustrated below.
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+ |  |
+ |  |
+ |  |
+ | |
| ECM 2001+ System | 45-2057 or 45-2058 HV Output Cable | 45-0088 Female/Female Adapters | 45-0204 Adapter Cables | 45-0503 Single Adapter Cable for Wand + selected Petri Dish Electrode |
USE OF PETRI DISH TISSUE SLICE ELECTRODES WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the Legacy ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below.
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+ |  |
+ | |
+ | |
+ | |
| Legacy ECM 2001 System | 45-0083 Coaxial Cables | 45-0088 Female/Female Adapters | 45-0204 Adapter Cables | 45-0503 Single Adapter Cable for Wand + selected Petri Dish Electrode |
Included Items
| Item # | Name | Included Items |
| 45-0500 | Tissue Slice Platinum Chamber Electrode Kit, 10 mm | Petri Dish Platinum Electrode Chamber, 10 mm and a Platinum Wand Electrode, 10mm, with micrograbber cable and single adaptor cable |
| 45-0490 | Tissue Slice Platinum Chamber Electrode Kit, 7 mm | Petri Dish Platinum Electrode Chamber, 7 mm and a Platinum Wand Electrode,7 mm, with micrograbber cable and single adaptor cable |
| 45-0502 | Platinum Electrode Wand Wand only, 10 mm | Requires Platinum Tissue Slice Chamber either 45-0491 or 45-0501 and cables 45-0503 and 45-0511 |
| Generator Compatibility | ECM 830, ECM 2001+, Legacy ECM 2001, and Gemini X2 |
| Voltage Range | 0 to 100 Volts |
| Pulse Length | 10 μs to 100 ms |
| Pulse Number | 1 to 99 (depending on voltage) |
| Electrode Material | Platinum |
| Wand Material | Platinum |
| Dimensions: | |
| Dish Electrode 7 mm (L x W x D) | 7 mm x 7 mm x 1 mm |
| Wand Electrode 7 mm (L x W x D) | 7 mm x 7 mm |
| Dish Electrode 10 mm (L x W x D) | 10 mm x 10 mm x 1 mm |
| Wand Electrode 10 mm (L x W x D) | 10 mm x 10 mm |
- Ex vivo electroporation
