Details
The Petri Dish Electrode is designed to be used with a 100 mm Petri dish that functions as the electroporation chamber. The Petri Dish Electrode is used to electroporate adherent cells or tissue grown in a petri dish.
To perform electroporation, simply add the exogenous molecule of interest into the electroporation buffer. The buffer can range in volume from 10 ml to 50 ml and is added to the cells grown in the plate. The electrode is lowered into the petri dish containing the sample and pulsed.
The electrode assembly has a 2 mm gap size. It contains parallel stainless steel electrodes which generate a homogeneous field. The Petri Dish Electrode is compatible with Gemini X2, ECM 630, ECM 830, ECM 2001+, and Legacy ECM 2001 Generators. The Petri Dish Electrode connects directly to Gemini X2, ECM 630, and ECM 830 generators. Please see table and connection diagram below for required cables and adapters for use with ECM 2001+ and Legacy ECM 2001.
REQUIRED CABLES
Required for connection to ECM 2001+ | |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-2057 or 45-2058 | ECM 2001+ HV Output Cable, 5 ft or 10 ft length |
Required for connection to Legacy ECM 2001 | |
45-0088 | Female/Female Adapter Set for Banana Plug Cables |
45-0083 | Coaxial to Banana Plug Cables, Red and Black, 10 ft |
USE OF PETRI DISH ELECTRODES WITH THE GEMINI X2 AND ECM 830 ELECTROPORATION SYSTEMS
Gemini X2 and ECM830 generators connect directly to the Petri Dish Electrode.
USE OF PETRI DISH ELECTRODES WITH THE ECM 2001+ ELECTROFUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001+ generator is connected using the sequence of cables and adapters illustrated below, then connected to the Petri Dish Electrode.
+ | + | |||
ECM 2001+ System | 45-0088 Female/Female Adapters + Petri Dish Electrode |
USE OF THE PETRI DISH ELECTRODES WITH THE LEGACY ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below, then connected to the electrode. This electrode is also compatible with the ECM 630, ECM 830, ECM 2001+ and Gemini X2 systems.
+ | + | |||
Legacy ECM 2001 System | 45-0083 Coaxial Cables | 45-0088 Female/Female Adapters + Petri Dish Electrode |
Generator Compatibility | ECM 630, ECM 830, ECM 2001+, Gemini X2, and Legacy ECM 2001 |
Voltage Range | 0 to 2000 V |
Volume Range | 10 to 50 ml |
Gap Size | 2 mm |
Autoclavable | No |
Field Type | Homogeneous |
Pulse Length | 10 µsec to 10 msec |
- Adherent mammalian cell transfections
- Plant tissue cell transfections