Tweezertrodes™ Electrodes are reusable non-invasive, tweezer-style in vivo and in utero electrodes for drug or gene delivery in animal tissues. Tweezertrodes consist of a standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes at the tip. The gap between the electrodes disks may be adjusted from under 1 mm to over 2 cm. Tweezertrodes are available in various sizes and two different metal alloys either Platinum or Stainless Steel. Platinum Tweezertrodes are available in 7 mm, 5 mm, 3 mm and 1 mm diameters and Stainless Steel are available as either 10 mm or 7 mm diameters. These electrodes connect to BTX generators using the Tweezertrode Connection cables (45-0204), and are compatible with the ECM 830, ECM 2001 and Gemini X2 generators. The Anode (+, red cable) of the Tweezertrode electrode corresponds to the side of the Tweezertrode with the blue adjusting screw on it.
Tweezertrodes may be cleaned with a mild detergent and sterilized with 70% ethanol or ethylene oxide.
APPLICATIONS & USE
Tweezertrodes may be used for many in vivo applications, including in utero and ex-vivo gene transfection, eletroporation therapy, and transdermal drug delivery. Following localized or systemic injection of the molecule of interest, the Tweezertrode electrode disks are used to grasp the tissue of interest. An electroporation pulse is then applied; initiating pore formation and incorporation of the molecule into the cells of the tissue in direct contact with the electrode disk.
Transgenic knockout mice were generated by transfecting directly into spermatogonal cells of mouse testies by in vivo electroporation Weili Shi and Majumdar et al. These authors demonstrated the potential and effectiveness of this technology to produce transgenic and knockout mice.1,5 Antonio and Mallamaci et. at., transfected mouse embryos in utero using these electrodes to target specific miRNAs to study regulation of these genes directly during neurogenesis.2 These electrodes and the ECM 830 were used to target GFP into progenitor olfactory cells to study the migration and differentiation during neruogensis of mouse brain in utero.3 The use of electroporation in adult Zebrafish provides a rapid way to study behavior and gene function in whole organism according to Madhusudhana T. et. al.4 Applications in utero transfection, transdermal drug delivery and electroporation therapy have been described.6,7,8
USE OF TWEEZERTRODES WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
Tweezertrodes electrodes are ideal for applications with the ECM 2001. The ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below. Using the ECM 830, all accessories plug directly into the unit.
|ECM 2001 System||45-0083 Coaxial Cables||45-0088 Female/Female Adapters||45-0024 Tweezertrode cable Adapters + Selected Tweezertrode Electrode|
|Voltage Range||0 - 200 VDC (do not use AC current)|
|Pulse Length Range||1 μsec – 200 msec|
|Pulse Number Range||1 to 99 (depending on voltage)|
|Operating Temperature||5oC - 40oC|
|Intended Use||Indoor use only|
|Relative Humidity||20 - 80%|
|Item #||Electrode Length||Electrode Diameter||Electrode Materials|
|45-0118||12 mm||7 mm||Stainless Steel|
|45-0119||12 mm||10 mm||Stainless Steel|
|45-0486||12 mm||1 mm||Platinum|
|45-0487||12 mm||3 mm||Platinum|
|45-0488||12 mm||7 mm||Platinum|
|45-0489||12 mm||5 mm||Platinum|
- In Vivo Drug or Gene Delivery
- Ex Vivo Drug or Gene Delivery
- In Utero Drug or Gene Delivery
1. Weili shi et al., Gernation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo. Acad Biochem Biophys Sci 2010, vol. 42, Issue 2, 116-121
2. Antonio and Mallamaci et al., Promotion of embryonic cortico-cerebral neurogenesis by miR-124. Nerual Development 2009-4:40.
3. Alexander T. Chesler et al. Selective Gene Expression by Postnatal Electroporation during Olfactory Intraneruon neruogenesis. PloS ONE 3(1): e1517. doi: 10.1371/Journal. Pone 0001517, 2008.
4. N. Nadhusudhana Rao et al. Electroporation of Adult Zebrafish, S.Li (ed) Electroporation Protocols preclinical and Clinical Gene Medicine. In Methods in Molecular Biology, Vol. 423 Humana Press 2008.
5. Dhup S. Majumdar et al. Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo. Nature Methods 2008.
6. D. DePietri Tonelli et al. Single-Cell Detection of Micro RNAs in Developing Vertebrate Embryos after Acute Administration of a Dual Fluorescence reporter/sensor plasmid. Biotechniques: 41: 727-732, 2006
7. Sato et al. Molecular Reproduction and Development, 61: 49-56 (2002).
8. Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001).