Description
This system incorporates features that make it the most technically advanced exponential decay electroporation system currently available. The generator utilizes the BTX Power Platform Technology design and novel digital user interface. The revolutionary Precision Pulse System provides the researcher unparalleled power in controlling the time constant. With the ability to deliver a maximum of 6000 A in the Low Voltage Mode, the ECM 630 is the most powerful generator in its class. Over 200 ECM 600 protocols may be duplicated with this instrument. The additional ECM 630 resistor selection "none" will allow researchers to reproduce protocols from competitive systems lacking resistor settings or reporting "unlimited" resistance. Indeed, the ECM 630 will perform the widest range of electroporation applications among commercial electroporators on the market.
Bacterial and Yeast Electroporation
The ECM 630 is the most flexible bacterial and yeast electroporator available, and will reproduce protocols by Beggs et al. for Mycobacterium avium 4; Sanders et al. for Haemophilus influenza and Haemophilus somnus 5; and Faber et al. for Hansenula polymorpha 6.
Mammalian Cell Transfections/ Protein/Drug Electroincorporation
Long duration electroporation, consistent with the ECM 630 platform, has resulted
in higher expression levels in COS-7, EDR3, DG44, and CV1 cell lines 1. The ECM
630 will duplicate the five-fold increase in transfection efficiency in comparison
to calcium phosphate precipitation for COS-7 reported by Ishmael et al.2 for the
ECM 600, as well as the murine T Lymphoma results reported by Sundback et al.3.
Plant Applications
Intact plant tissue and protoplasts may be transformed using the ECM 630, including
the species Agrostis palustris (creeping bentgrass) and Lycopersicon esculentum
(tomato) 7, as well as tobacco pollen transformation8.
Other Cell Types
Exponential decay electroporation has been demonstrated to be superior to microinjection
for medaka oocytes 9. Yee et al., transfected and expressed firefly luciferase in
Giardia lamblia 10. In addition, Quillan et al. electrotransfected Xenopus laevis
melanophores and fibroblasts 11.
Specialty Electrode Applications: The BTX Choice
Takacs directed the electroporation of COS-7 in a 96-well plate format using single,
8-well and 96-well coaxial electrodes 12, while Parham et al. optimized transient
gene expression in CHO, CV-1, COS-7, HEK 293, and NSO using the BTX Flow Through
Electroporation System 13. The coaxial electrodes have now been replaced by BTX High Throughput 96 Well Electroporation plate.
In Vivo Applications
Exponential decay generators may be used for a variety of animal in vivo applications,
including drug delivery to pig carotid artery 14 and transdermal delivery of large
molecules 15.
| Standard Capabilities |
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Interface: |
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Digital User Interface |
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Input: |
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110 V/220 V Universal |
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Charge Time: |
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5 sec maximum |
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Voltage Range: |
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10 - 500 V LV Mode/ 1 V resolution
50 - 2500 V HV Mode/ 5 V resolution
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Capacitance Range: |
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1 µF, 25 µmF - 3275 µmF LV Mode/25 µmF resolution
1 µmF, 25 µmF, 50 µmF HV Mode
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Resistance Range: |
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25 ohm - 1575 ohm/ 25 ohm resolution HV & LV Modes
"None" setting to simulate alternative systems lacking resistance control LV Mode
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Precision Pulse™ |
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Over 8500 RC Time Constants LV Mode
284 RC Time Constants HV Mode
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Safety: |
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Short Circuit Proof
Maximum Pulse Delay 10 s
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| Other Electrical Characteristics |
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Amperage: |
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6000 A limit LV Mode
3000 A limit HV Mode
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| Physical Characteristics |
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Footprint: |
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12.5" x 12.25" x 5.5" (14 x 31.8 x 31.1 cm) (W x D x H)
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Weight: |
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10 lbs. (4.5 kg)
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Display: |
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20 x 4 character LCD
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Controls: |
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Single Rotary Encoder with push button toggle between all set parameters.
Additional on/off Power and Start switches
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| Monitoring |
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Monitoring and display of Vpeak and RC Time Constant
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System
| Catalog # |
Model |
|
Description |
| 45-0001 |
6300 |
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ECM 630 Electroporation System
ECM 630 Generator with Power Supply
Model 630B Safety Stand
Model 610, 620, 640 Disposable Electroporation Cuvettes Plus™, 10 each
Model 660 Cuvette Rack
Manual and Instruction Sheets
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| Catalog # |
Model |
|
Description |
| 45-0124 |
610 |
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Cuvettes Plus, 1 mm gap, 50/pkg
|
| 45-0125 |
620 |
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Cuvettes Plus, 2 mm gap, 50/pkg
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| 45-0126 |
640 |
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Cuvettes Plus, 4 mm gap, 50/pkg
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- Bodwell et al., Journal of Steroid Biochemistry and Molecular Biology, 68: 77-92 (1999)
- Ishmael, et al., Journal of Neurochemistry, 67 (4): 1500-1510 (1996)
- Sundback et al., Journal of of Immunology, 5971-5978 (1998)
- Beggs et al., Journal of Bacteriology, 177 (17): 4836-4840 (1995)
- Sanders et al., FEMS Microbiology Letters, 154 (2): 251-258 (1997)
- Faber et al., Current Genetics, 25 (4): 305-310, (1994)
- Lin et al., Plant Physiology and Biochemistry, 35 (12): 959-968 (1997)
- Smith et al., Plant Science, 104: 49-58 (1994)
- Chen et al., Zoological Studies, 34 (4): 215-234 (1995)
- Yee, and Nash, T.E., PNAS, 92 (12): 5615-5619 (1995)
- Quillan et al., PNAS, 92: 2894-2898 (1995)
- Peterfy et al., Methods in Molecular and Cellular Biology, 5 (6): 353-362 (1995)
- Parham et al., Cytotechnology, 28: 1-9 (1999)
- Cui, Journal of the American College of Cardiology, 29: 201a (1997)
- Zhang, et al., Bioelectrochemistry and Bioenergetics, 42: 283-292 (1997)
This product meets the requirements of the European Communities (EC),
and complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.
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