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Description
The ECM 399 is an electroporation system specifically designed to produce the precise
field strengths and pulse lengths required for the transfection of mammalian and
transformation of bacterial and yeast cells. The ECM 399 utilizes the new BTX Power
Platform Technology design and novel digital user interface. The single-dial
encoder simplifies the operation of ECM 399. Portable, convenient, and
user-friendly are major features of this unit.
Bacteria/Yeast Transformation
In the High Voltage (HV) Mode, the ECM 399 is optimized to electroporate E. coli
and other bacteria and yeast for construction of cDNA libraries or mutagenesis.
Mammalian Cell Transfection
In the Low Voltage (LV) Mode, the ECM 399 is designed to deliver a pulse length
of approximately 26 msec using the 4mm gap cuvette, at a volume of 400µl using
conductive media such as PBS. This protocol has been found to be ideal for
transfection of lymphoid, myeloid, glial, fibroblast and Cos 7 cells.1 Protocols
for these and other mammalian cells can be optimized by varying the voltage
and/or the pulse length. Adjusting the voltage adjustment knob varies the voltage,
while the pulse length may be changed by varying the sample volume or buffer composition.
| Standard Capabilities |
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| Operational Status: |
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Internal Self Test Upon Start-up |
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Interface: |
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Digital User Interface |
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Charge Time: |
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5 sec maximum |
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Voltage Range: |
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2-500 V LV Mode/ 2 V resolution
10-2,500 V HV Mode/10 V resolution
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Capacitance: |
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1050 µF LV Mode
36 µF in HV Mode
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Resistance: |
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150 ohms both LV and HV Modes
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Safety: |
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Short circuit and arc proof
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| Physical Characteristics |
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Footprint: |
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9.1"x7.7"x4.3"(WxDxH)
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Weight: |
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7 pounds
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Display: |
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16-character LCD backlit
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Controls: |
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Single rotary encoder for voltage
selection. Additional on/off power and start switches
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| Monitoring |
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Monitoring and display of V p and RC time constant |
System
| Catalog # |
Model |
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Description |
| 45-0000 |
399 |
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ECM 399 Electroporation System
ECM 399 Generator with
Power Supply, one PEP™ (Personal
Electroporation Pak), 20 Disposable
"Universal" cuvettes (10: 1mm; 10: 4mm)
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| Catalog # |
Model |
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Description |
| 45-0212 |
PEP-BLU |
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Personal Electroporation Pak (PEP)
"plug-in" cuvette module |
| 45-0214 |
610 |
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Cuvettes Plus™ (1mm Gap)
sterile package of 50 |
| 45-0215 |
620 |
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Cuvettes Plus™ (2mm Gap)
sterile package of 50 |
| 45-0216 |
640 |
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Cuvettes Plus™ (4mm Gap)
sterile package of 50 |
1. C. Baum et al., "An Optimized Electroporation Protocol Applicable to a
Wide Range of Cell Lines", BioTechniques vol. 17(6): 1058-1062, (1994).
This product meets the requirements of the European Communities (EC),
and complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.
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