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Product Information Technical Specifications (PDF, 23KB) Product Manual (PDF, 1MB)

Applications

Mammalian Cell Transfections/Gene Therapy
The use of square wave pulses for the transfection of mammalian cells is well established. Fakhrai has used the BTX T 820 in the technique of Electro-Immuno Therapy.¹ The ECM 830 will enable transfection of mammalian cells while maintaining higher cell viability.² For many mammalian cells, higher transfection efficiencies will also be obtained.³

Mammalian Cell Protein/Drug Electroincorporation
Square wave pulses have been used to electroincorporate a variety of molecules into mammalian cells; Tsong has used square wave to load drugs into erythrocytes⁴ while Marrero has used the BTX T 820 and BTX Model 366 Petri Dish Electrode to incorporate biologically active antibodies into RASM cells.⁵

In Vivo Applications
Recent advances by BTX have allowed researchers to perform in vivo electroporation. In vivo Electro Gene Therapy has significant advantages over viral mediated gene transfer, biolistics, and the use of cationic lipids.^6,7,8

Nuclear Transfer
BTX square wave systems have been used by the experts in mammalian embryo manipulation techniques in nuclear transfer for applications ranging from pharmaceutical production to organ generation. ^9,10,11

Plant Bacterial and Yeast Applications
Intact plant tissue, protoplasts and competent bacteria and yeast may be transformed using the ECM830¹². References indicate that square wave is competitive with exponential decay for plant protoplast bacteria and yeast transformation.¹³

Bacterial and Yeast Electroporation
The ECM 830 is capable of reproducing our T 820 bacterial protocols, including all four E coli electrotransformation protocols referencing transformation efficiencies equivalent or superior to exponential decay results.¹⁴

Technical Specifications

Standard Capabilities
	Interface:		Digital User Interface
	Input:		110 V/220 V Universal
	Charge Time:		13 sec maximum
	Voltage Range:		5 - 500 V LV Mode/ 1 V resolution 505 - 3000 V HV Mode/ 5 V resolution
	Pulse Length Range:		10 ms - 999 ms LV Mode/ 1 ms resolution 1 µsec - 999 msec LV Mode/ 1 µsec resolution 1 sec - 10 sec LV Mode/ 0.1 sec resolution 10 µs - 600 µs HV Mode/ 1 µs resolution
	Multiple Pulsing:		1 - 99 pulses
	Pulse Interval:		100 msec - 10 sec
	Programmability:		Storage for 3 setups (V, t, n, interval)
	Arc Control:		Arc Quenching
Other Electrical Characteristics
	Capacitance:		4000 µF LV, 110 µF HV
	Amperage:		500 A LV, 100 A HV
Physical Characteristics
	Footprint:		12.5" x 12.25" x 5.5" (W-D-H)
	Weight:		15 lbs. (6.8 kg)
	Display:		20 x 4 character LCD
	Controls:		Single Rotary Encoder with push button toggle between all set parameters. Additional on/off Power and Start switches
Monitoring:			Displays all set parameters after pulsing
Remote Operation:			Footswitch available. Please contact BTX Technical Services at btxinfo@harvardapparatus.com,1-800-272-2775, 508-893-8999, techsupport.btx@harvardapparatus.com for assistance.

Ordering Information

Accessories

References

1. Fakhrai, H. et al., PNAS, 93: 2909- 2914 (1996)
2. Pasco, David, University of Mississippi, Natural Products Center, Personal Communication and Protocols PR0296-0298, PR0345-349, PR0351-355 (1998)
3. Takahashi, M. et al., Leukemia Research, 15 (6): 507-513 (1991)
4. Tsong, T., et al., Biblthca Haemat, 51: 108-114 (1985)
5. Marrero, M., et al., The Journal of Biological Chemistry, 270 (26): 15734-15738 (1995)
6. Miramatsu et al., International Journal of Molecular Medicine, 1: 55-62 (1998)
7. Nishi, T., et al., Cancer Research, 56: 1050-1055 (1996)
8. Heller, R., et al., FEBS Letters, 389: 225-228 (1996)
9. Cambell, K., PPL Therapeutics, Roslin, Scotland, Personal Communication (1998)
10. Cibelli, Jose et al., Science, 280: 1256-1258 (1998)
11. Meng, J., et al., Biology of Reproduction, 57: 454-459 (1997)
12. Lin, C., et al., Plant Physiol. Biochem., 35 (12): 959-968 (1997)
13. Saunders, J., Guide to Electroporation and Electrofusion, 227-247 (1991)
14. BTX Protocols PR0182, PR0183, PR0336, PR0342 (1998)
15. Costaglioli, P., et al., Current Genetics, 27 (1): 26-30 (1994)

This product meets the requirements of the European Communities (EC), and complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.