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ECM 630 Exponential Decay Wave Electroporation System

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ECM 630 Exponential Decay Wave Electroporation System
Electroporation system primarily used for bacteria and yeast transformation. High throughput (25- and 96-well) models available.


Product Item # Price  
ECM 630 Electroporation System Complete, for Cuvettes (N. America) 45-0001   View Price
ECM 630 Electroporation System Complete, for Cuvettes 45-0001INT   View Price
ECM 630 Generator Only (N. America) 45-0051   View Price
ECM 630 Generator Only 45-0051INT   View Price
ECM 630 High Throughput Electroporation System, 25-well with HT-100 (N. America) 45-0412   View Price
ECM 630 High Throughput Electroporation System, 25-well with HT-100 45-0412INT   View Price
ECM 630 High Throughput Electroporation System, 96-well with HT-100 (N. America) 45-0422   View Price
ECM 630 High Throughput Electroporation System, 96-well with HT-100 45-0422INT   View Price
ECM 630 Electroporation System Complete, for Cuvettes with Monitoring (N. America) 45-0071   View Price
ECM 630 Electroporation System Complete, for Cuvettes with Monitoring 45-0071INT   View Price
ECM 630 High Throughput Electroporation System with Monitoring (N. America) 45-0471   View Price
ECM 630 High Throughput Electroporation System with Monitoring 45-0471INT   View Price
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Details

The ECM 630 is an electroporation system primarily used for bacteria and yeast transformation in cuvettes. High throughput (25- and 96-well) models available.

ECM 630 GENERATOR

The ECM 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. The ability to select the resistance and capacitance values, and adjust the range of voltages is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection.  The ECM 630 can perform the widest range of electroporation applications among commercially available electroporation generators.

Flexibility is important, so BTX has designed a plug and play system for our ECM 630 system to transition between standard cuvettes and to 25/96-well plates using High Throughput Plate Handlers.

The ECM 630 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

FEATURES

  • Utilizes the BTX Power Platform Technology design and novel digital user interface
  • Precision Pulse Technology provides unparalleled power in controlling the time constant
  • Delivers a maximum of 6,000 A in the Low Voltage Mode; the ECM 630 is the most powerful generator in its class
  • Voltage range of 10 V to 500 V with 1 V resolution and 1 µF, 25 µF to 3,275 µF in 25 µF increments. 25 µF to 1,575 µF, 25 µF resolution with "None" setting
  • Voltage range of 50 V to 2,500 V with 5 V resolution and either 25 µF or 50 µF, 25 µF to 3,275 µF with 25 µF resolution
  • Resistance range of 25 ohm to 1,525 ohm with 25 ohm resolution. The additional resistor selection "None" allows researchers to reproduce protocols from competitive systems
  • Hundreds of BTX Electroporation protocols may be duplicated with this instrument

APPLICATIONS

  • Transformation of bacteria and yeast
  • Transfection of mammalian cells
  • Transfection of plant tissue and plant protoplasts
  • High throughput 25- and 96-well electroporation

HIGH THROUGHPUT (HT) ELECTROPORATION

The High Throughput (HT) 96- and 25-well systems offer multi-well electroporation technology for processing multiple samples in seconds. Using the HT multi-well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.

The key to the HT System is the combination of the ECM 630 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 630 generator. The HT 100 Plate Handler provides manual track switching (45-0412, 45-0422).

MONITORING OPTION

Addition of the ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment.

INCLUDED ITEMS

Item #ProductIncluded Items
45-0001 ECM 630 Electroporation System Complete, for Cuvettes (N. America) ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0001INT ECM 630 Electroporation System Complete, for Cuvettes ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0051 ECM 630 Generator Only (N. America) ECM 630 Generator only
45-0051INT ECM 630 Generator Only ECM 630 Generator only
45-0412 ECM 630 High Throughput Electroporation System, 25-well with HT-100 (N. America) ECM 630 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0412INT ECM 630 High Throughput Electroporation System, 25-well with HT-100 ECM 630 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0422 ECM 630 High Throughput Electroporation System, 96-well with HT-100 (N. America) ECM 630 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0422INT ECM 630 High Throughput Electroporation System, 96-well with HT-100 ECM 630 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a Plate Adaptor
45-0071 ECM 630 Electroporation System Complete, for Cuvettes with Monitoring (N. America) ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0071INT ECM 630 Electroporation System Complete, for Cuvettes with Monitoring ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0471 ECM 630 High Throughput Electroporation System with Monitoring (N. America) ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-100 Plate Handler and a Plate Adaptor
45-0471INT ECM 630 High Throughput Electroporation System with Monitoring ECM 630 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660 , ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-100 Plate Handler and a Plate Adaptor

 SPECIFICATIONS FOR THE ECM 630 GENERATOR

Display Type 20-character by 4-line liquid crystal display. LED backlit 
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
Fusing
2.5 A, T rating 250 V 
Environmental Characteristics
Intended Use Indoor user only
Operating Temperature 10° C to + 40° C
Cooling Convection through metal case
Relative Humidity 60%
Altitude 2,000 m (operating)
Mechanical Characteristics 
Footprint 12.5" x 12.25" x 5.5" (W x D x H)
Weight 10.0 lb (4.5 kg)
Controls Single rotary knob with push button toggle to set parameters, On/Off power and Start switches

     

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V / 220 V Universal
Charge Time 5 sec maximum
Arc Control Arc Quenching™
Voltage Range 10 - 500 V LV Mode / 1 V resolution 50 - 2500 V HV Mode/ 5 V resolution
Capacitance Range 1 µF, 25 µF to 3,275 µF LV Mode 25 µF, 50 µF HV Mode
Resistance Range 25 ohm - 1,575 ohm/ 25 ohm resolution HV & LV Modes; "None" setting to simulate alternative systems lacking resistance control LV Mode

  • Transformation of bacteria and yeast
  • Transfection of mammalian cells
  • Transfection of plant cells & plant protoplasts
  • High throughput 25/96-well
Item # Product
45-0466 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, coated, 1 plate
45-0467 25-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, pkg. of 6
45-0462 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, 1 plate
45-0463 25-Well Disposable Electroporation Plate, 4 mm gap, 250 µl, pkg. of 6
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, Coated, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 250 µl, 1 plate
45-0530 Adherent Cell Electrode, 5 mm gap
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125INT Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0400INT HT 100 Plate Handler, Manual Track Switching
45-0400 HT 100 Plate Handler, Manual Track Switching (N. America)
45-0401INT HT-200 Plate Handler, Auto-Sense Track Switching
45-0401 HT-200 Plate Handler, Auto-Sense Track Switching (N. America)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)

ECM 630 User's Manual

 

ECM-630 Technical Specifications

 

REFERENCES

 

James Weger-Lucarelli, Nisha K. Duggal, Kristen Bullard-Feibelman, Milena Veselinovic, Hannah Romo, Chilinh Nguyen, Claudia Rückert, Aaron C. Brault, Richard A. Bowen, Mark Stenglein, Brian J. Geiss, Gregory D. Ebel. Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone. J. Virology. 2016.

 

Alba Fernández-Sanlés, Beatriz Berzal-Herranz, Rodrigo González-Matamala, Pablo Ríos-Marco, Cristina Romero-López and Alfredo Berzal-Herranz. RNA Aptamers as Molecular Tools to Study the Functionality of the Hepatitis C Virus CRE Region. Molecules. 2015, 20, 16030-16047.

 

Yu-Tze Horng, Wen-Yih Jeng, Yih-Yuan Chen, Che-Hung Liu, Horng-Yunn Dou, Jen-Jyh Lee, Kai- Chih Chang, Chih-Ching Chien. Analysis of Codon 548 in the rpoB Gene Involved in Mycobacterium tuberculosis Resistance to Rifampin. Antimicrobial Agents and Chemotherapy. March 2015 Volume 59.

 

Jessica A. Wisniewski, Wee L. Teng, Trudi L. Bannam, Julian I Rood. Two Novel Membrane Proteins, TcpD and TcpE, Are Essential for Conjugative Transfer of pCW3 in Clostridium perfringens. Journal of Bacteriology. February 2015.

 

Kurth, M. et al. Reporter gene expression in cell culture stages and oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa).Parasitology Research. 2009.

   

Lama, A. et al. Glyceraldehyde-3-Phosphate Dehydrogenase Is a Surface- Associated, Fibronectin-Binding Protein of Trichomonas vaginalis. Infection & Immunity2009.

 

Thyagarajan, B. et al. Creation of Engineered Human Embryonic Stem Cell Lines Using phiC31 Integrase. Stem Cell2008.

 

Donoho, G. et al. Analysis of Gene Targeting and Intrachromosomal Homologous Recombination Stimulated by Genomic Double-Strand Breaks in Mouse Embryonic Stem CellsMolecular & Cellular Biology.1998.