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ECM 2001 Electro Cell Fusion & Electroporation System

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ECM 2001 Electro Cell Fusion & Electroporation System
Multi-purpose electro cell manipulation generator capable of producing a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.
Product Item # Price  
ECM 2001 Cell Fusion System, Complete (N. America) 45-0010   View Price
ECM 2001 Cell Fusion System, Complete 45-0010INT   View Price
ECM 2001 Electroporation System Complete, for Cuvettes (N. America) 45-0011   View Price
ECM 2001 Electroporation System Complete, for Cuvettes 45-0011INT   View Price
ECM 2001 Embryo Manipulation System (N. America) 45-0012   View Price
ECM 2001 Embryo Manipulation System 45-0012INT   View Price
ECM 2001 Hybridoma Production System (N. America) 45-0018   View Price
ECM 2001 Hybridoma Production System 45-0018INT   View Price
ECM 2001 Generator Only (N. America) 45-0080   View Price
ECM 2001 Generator Only 45-0080INT   View Price
ECM 2001 Electroporation System Complete, for Cuvettes with Monitoring (N. America) 45-0013   View Price
ECM 2001 Electroporation System Complete, for Cuvettes with Monitoring 45-0013INT   View Price
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Details

ECM 2001 GENERATOR

The ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

The ECM 2001 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

FEATURES

  • Wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 µsec to 10 sec
  • Arc quenching
  • Digital display of actual voltage and pulse length delivered

FUSION

The ECM 2001 is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Electrofusion is carried out by applying both the AC and DC pulse waveforms. Microsecond switch-over time from AC to DC allows for highly efficient fusion. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids. The 1 MHz AC pulse aligns cells together in real time, saving time (seconds vs. hours) in comparison to PEG methods. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.

ELECTROPORATION

The ECM 2001 EP System is designed for transfection of eukaryotic cells in suspension using electroporation cuvettes

Utilizing the ECM 2001 for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab. 

ADHERENT CELL TRANSFECTION

Electroport adherent cells directly into the dish used for cell growth. The ECM 2001 coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

HYBRIDOMA PRODUCTION

For use in hybridoma production applications, the ECM 2001 generator, 3.2 mm gap or 10 mm gap Microslides, and connection cables are required. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience.

Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids.

INCLUDED ITEMS

Item #ProductIncluded Items
45-0010 ECM 2001 Cell Fusion System Complete (N. America) ECM 2001 Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0010INT ECM 2001 Cell Fusion System Complete ECM 2001 Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0011 ECM 2001 Electroporation System Complete, for Cuvettes (N. America) ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0011INT ECM 2001 Electroporation System Complete, for Cuvettes  ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0012 ECM 2001 Embryo Manipulation System (N. America) ECM 2001 Generator, Microslides, round wire (0.5 mm gap,1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-0012INT ECM 2001 Embryo Manipulation System ECM 2001 Generator, Microslides, round wire (0.5 mm gap,1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-0018 ECM-2001 Hybridoma Production System (N. America) ECM 2001 Generator, Microslides, rectangular wire (3.2 mm gap, 10 mm gap), Adapter Set, Micrograbber adapter cables, BTX Cytofusion Medium C, 500 ml, Safety Stand for Flatpack
45-0018INT ECM-2001 Hybridoma Production System ECM 2001 Generator, Microslides, rectangular wire (3.2 mm gap, 10 mm gap), Adapter Set, Micrograbber adapter cables, BTX Cytofusion Medium C, 500 ml, Safety Stand for Flatpack
45-0080 ECM 2001 Generator Only (N. America) ECM 2001 Generator only
45-0080INT ECM 2001 Generator Only ECM 2001 Generator only
45-0013 ECM 2001 System Complete, for Cuvettes with Monitoring  (N. America) ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0013INT ECM 2001 System Complete, for Cuvettes with Monitoring  ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables

AC PARAMETERS (ALIGNMENT)

Proprietary nonsinusoidal wave shape
Frequency 1 MHz
Voltage 0 - 75 V ; RMS (zero to peak)
Duration 0 - 99 sec
Post fusion AC 1/10 of pre-fusion amplitude
Post fusion ramp 1 - 9 sec
Pause between AC/DC 50 µsec

 

DC PULSE PARAMETERS (FUSION) (ELECTROPORATION)

High Voltage Mode
Voltage 10 - 3,000 V
Pulse Length 1 - 99 µsec/1 µsec resolution
Low Voltage Mode
Voltage 10 - 500 V
Pulse Length 0.01 - 0.99 msec/ 0.01 msec res ,1 - 99 msec/ 1 msec res
Number of Pulses 1 - 9 pulses
Pulse Interval 1 sec fixed
Number of Post Cycle Repeats 0 - 9 cycles
Capacitance LV Mode: 3,900 µF / HV Mode: 108 µF
Resistance LV Mode: 50 ohms / HV mode: 50 ohms
Safety Generator is short-circuit proof
Remote Operation Automatic or manually controlled switch

 

MECHANICAL CHARACTERISTICS

Footprint 17' x 15.5" x 11" ( W x D x H)
Weight 47 lb (21.3 kg)
Controls Thumbwheel potentiometer and push button control
  • Cell fusion
  • Nuclear transfer
  • Embryo manipulation
  • Hybridoma production
  • Mammalian cell transfection
  • Plant protoplast fusion
  • Stem cell production
Item # Product
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume, pkg. of 10
47-0001 BTXpress Cytofusion Medium C, 500 ml volume
47-0003 BTXpress Cytoporation Low Conductivity Medium T4, 500 ml volume
47-0010 5 ml Coaxial chamber for production
47-0020 9 ml Coaxial Chamber for production
47-0030 2 ml Coaxial Chamber for optimization
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125INT Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0087 ECM 2001 Adaptor Set, Micrograbber to Banana Plug Cables
45-0217 ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)
45-0086 ECM 2001 CE Footswitch with 45-0085 cable
45-0088 ECM 2001 Female/Female Adapter Set for Banana Plug Cables (Use with 45-0216)
45-0214 ECM 2001 Footswitch (for non-CE 2001 only)
45-0085 ECM 2001 Footswitch Cable
45-0216 ECM 2001 Micrograbber to Banana Plug Connection Cables, Red and Black, 10 ft (for use with microslides, genetrodes and tissue petri dish)
47-0208 ECM 2001 Safety Stand for Flatpack
45-0059 Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America)
45-0108 Flat Electrode for Cell Fusion
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0107 Meander Fusion Chamber
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0100 Petri Dish Electrode
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0130 Petri Pulser
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

ECM 2001 User's Manual

 

ECM 2001 Technical Specifications

 

Cell Fusion Applications: Electrofusion vs. PEG

 

REFERENCES

 

Natalie J. Thornburg, Heng Zhang, Sandhya Bangaru, Gopal Sapparapu, Nurgun Kose, Rebecca M. Lampley, Robin G. Bombardi, Yingchun Yu, Stephen Graham, Andre Branchizio, Sandra M. Yoder, Michael T. Rock, C. Buddy Creech, Kathryn M. Edwards, David Lee, Sheng Li, Ian A. Wilson, Adolfo García-Sastre, Randy A. Albrecht, and James E. Crowe Jr. H7N9 influenza virus neutralizing antibodies that possess few somatic mutations. The Journal of Clinical Investigation, April 2016.

 

Clow A., Gaynor P., Oback, B. A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusionBiomed Microdevices. 12, 777-786 (2010) . 

 

Green, A.M., Wells, D.N., Oback, B. Cattle cloned from increasingly differentiated muscle cellsBiol Reprod. 77, 395-406 (2007).     

 

Young Chung, Sandy Becker., Embryonic Stem Cells Using Nuclear Transfer. Methods in Enzymology., Volume 418, 2006.

 

Kenichiro, I. et al. Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique. INTERNATIONAL JOURNAL OF ONCOLOGY 29: 531-539, 2006.

 

Nakamura, Y. et al. Phospholipase C-σ1 and σ3 Are Essential in the Trophoblast for Placental Development. Molecular And Cellular Biology,(2005).

 

Soares, M.L. et al. Functional Studies for Signaling Pathways in Peri-implantation development of the Mouse Embryo by RNAi. BMC Developmental Biology,(2005).

 

Bedford, S. J. et al. Patterns of Intracellular Calcium Oscillation in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible Explanations for the Low Success of this Assisted Reproduction Technique in the HorseBiology of Reproduction, (2004).

 

Yul, Z. et al. Specific Antitumor Effects for Tumor Vaccine Produced by Electrofusion between Osteosarcoma Cell and Dendritic Cell in Rat. Cellular & Molecular Immunology, (2004).

 

Grabarek, J.B. et al. Efficient Delivery of dsRNA into Zona-Enclosed Mouse Oocytes and Preimplantation Embryos by ElectroporationGenesis, (2002).

 

Liu, H. et al. Reconstruction of Mouse Oocytes by Germinal Vesicle Transfer: Maturity of Host Oocyte Cytoplasm Determines MeiosisHuman Reproduction,(1999).