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Description
The ECM 2001 is a versatile electro cell manipulation instrument. It generates an AC waveform for benign dielectrophoretic alignment of cells. With only microsecond switch-over time from AC to DC, efficient fusion can take place. After fusion AC re-application keeps cells together for the rounding off process. The DC square pulse section can be used as a stand alone powerful electroporation system with a wide range of voltages and pulse lengths capable of operating into low impedance loads.
Somatic cell nuclear transfer technique is applicable for the production of stem cells. The nuclear transfer between an oocyte and a donor cell is accomplished by cell fusion using electrofusion technique. An electrical pulse is applied thus fusing the oocyte and the donor cell. Resulting blastocyst can be further modified to produce immunologically compatible stem cells. Also called the Roslin Technique, it is applicable for use on a variety of animal cells.
- Versatile Electro Cell Fusion & Electroporation Instrument
- Microprocessor Controlled Precision
- Wide Range of Parameters
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| AC Parameters (Alignment) |
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Frequency |
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1 MHz, fixed |
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Voltage |
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0-75 VRMS |
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Duration |
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0-99 sec |
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Post fusion AC amplitude |
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1/10 of prefusion amplitude |
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Post fusion AC duration |
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0-9 sec |
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Pause between AC / DC |
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50 µsec |
| DC Pulse Parameters (Fusion/Electroporation) |
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High Voltage Mode (HV mode) |
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Voltage |
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500-3000 V |
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Pulse Length |
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1-99 µsec |
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Low Voltage Mode (LV mode) |
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Voltage |
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10-500 V |
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Pulse Mode |
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0.01-0.99 msec
1-99 msec
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| Number of Pulses |
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0-9 |
| Additional Features |
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Automatic or manually controlled sequence
Number of sequence repeats 1-9
Remote control available (sold separately)
Size: 17"W-11"H-15.5"D (43.2 x 28 x 39.4 cm)
Warranty: 2 years
Can operate into a 20 Ohm load
Short circuit and arc proof
Power: 100/115 VAC, single phase
230 VAC, single phase
Fuse: 7A slow blow
Weight: 47 lbs. (22 kg)
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System
| Model |
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Available Configurations |
| 2001EP |
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ECM® 2001 Electroporation System |
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Electro Cell Manipulator® 2001
Disposable Cuvettes - 1mm, 2mm and 4mm gap sizes - sterile package of 30
630B Safety Stand - for use with disposable cuvettes
Cuvette Rack - 20 numbered positions
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| 2001EM |
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ECM® 2001 Embryo Manipulation System
Electro Cell Manipulator® 2001
Microslide 450: - 0.5mm gap, 20µl - Package of 10
Microslide 450: - 1.0mm gap, 40µl - Package of 10
Microslide 453: - 3.2mm gap, 650µl - Package of 1
Micrograbber Cables 464 - for use with Microslide 450
Square-post Micrograbber Cables 465 - for use with Microslide 453
Coaxial Banana Plug Cable 5343 - 10 ft.
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| 2001EF |
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ECM® 2001 Cell Fusion System
Electro Cell Manipulator® 2001
Fusion Chambers
Microslide 450 - 0.5mm gap, 20µl - Package of 10
Microslide 453 - 3.2mm gap, 650µl - Package of 1
Micrograbber Cables 464 - for use with Microslide 450
Square-post Micrograbber Cables 465 - for use with Microslide 453
Meander Fusion Chamber 454 - 0.2mm gap on glass microslide (includes connector) - package of 4
Flat Electrode/Divergent Field 484 - 1.0mm gap, 0.5ml Divergent Field Geometry
Electrode Adapter 466 - for use with Flat Electrode 484
Coaxial Banana Plug Connection Cable - 10 ft. 5343
Electroporation Chambers
Disposable Cuvettes - 1mm, 2mm and 4mm gap - Sterile package of 30
630B Safety Stand - for use with Disposable Cuvettes
Cuvette Rack - 20 numbered positions
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| Model |
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Description |
| 610 |
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Cuvettes Plus, 1 mm gap, 50/pkg
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| 620 |
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Cuvettes Plus, 2 mm gap, 50/pkg
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| 640 |
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Cuvettes Plus, 4 mm gap, 50/pkg
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