Tweezertrodes are reusable, tweezer-style in vivo electrodes for drug or gene delivery in animals. Tweezertrodes consist of a standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes at the tip. The gap between the electrode disks may be adjusted from under 1 mm to over 2 cm. Tweezertrodes are available in two sizes for stainless stell, 10mm and 7mm diameters. Our Platinum Tweezertrodes are available in 7mm, 5mm, 3mm and 1mm diameters. Tweezertrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. These electrodes connect to an electroporator with the Model 524 Connection Cable, and are compatible with the BTX ECM 630, 830 and 2001.
Tweezertrodes may be used for many in vivo applications, including gene
and drug delivery. Following localized or systemic injection of the
molecule of interest, the Tweezertrode electrode disks are used to
grasp the tissue of interest. An electroporation pulse is then given,
initiating pore formation and incorporation of the molecule into the
cells of the tissue in direct contact with the electrode disks.
Suzuki et al. used Tweezertrodes to deliver a Green Fluorescent
Protein (GFP) gene into rat liver. The authors demonstrated successful
gene transfer and expression in situ.1,2 Saito and Nakatsuji used a
similar electrode to introduce plasmid DNA during ex-utero and in-utero
electroporation.7 Sato et al. used the Tweezertrode with the T820
to introduce EGFP expressing plasmid in mouse testis.8 The Tweezertrodes can be used to reproduce applications requiring the caliper electrodes, including gene therapy, transdermal drug delivery and electroporation therapy.3,4,5,6
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| Standard Capabilities |
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Voltage Range: |
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0 - 200 VDC (Do not use AC current) |
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Pulse Length Range: |
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1 µsec - 200 msec |
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Pulse Number Range |
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1 to 99 (depending on voltage) |
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Operating Temperature |
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5Ëš to 40ËšC |
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Intended Use |
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Indoor use only |
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Relative Humidity |
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20 to 80% |
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Masimum Altitude |
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2,000 m (6,562 ft) |
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Pollution Degree |
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II |
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Insulation Category |
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CAT 1 |
| Physical Characteristics |
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| Order No. |
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Electrode
Length |
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Electrode
Diameter |
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Electrode
Materials |
| 45-0118 |
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12 mm |
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7 mm |
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Stainless Steel |
| 45-0119 |
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12 mm |
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10 mm |
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Stainless Steel |
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| Compatibility |
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Generators |
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ECM 630, 830 and 2001 |
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Monitoring |
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The Enhancer 3000 Monitoring System recommended |
| Order No. |
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Model |
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Description |
| 45-0165 |
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520KIT |
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Tweezertrode Kit, 7 mm, Includes Cable |
| 45-0118 |
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520 |
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Tweezertrode Electrode, 7 mm Diameter |
| 45-0119 |
|
522 |
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Tweezertrode Electrode, 10 mm Diameter |
| 45-0166 |
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522KIT |
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Tweezertrode Kit, 10 mm, Includes Cable |
| 45-0204 |
|
524 |
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Tweezertrode Connection Cable |
| 45-0059 |
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ENHANCER3000SC |
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The Enhancer 3000 Monitoring System |
| 45-0486 |
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Platinum Tweezertrode, 1 mm Diameter, Includes Cables |
| 45-0487 |
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Platinum Tweezertrode, 3 mm Diameter |
| 45-0488 |
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Platinum Tweezertrode, 7 mm Diameter |
| 45-0489 |
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Platinum Tweezertrode, 5 mm Diameter |
| 45-0204 |
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Tweezertrode Cables |
- Suzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivo Electroporation, FEBS Letters, 425: 436-440 (1998)
- BTX Electroporation Protocol PR0363 (1998)
- Zhang et al., In vivo transdermal delivery of large molecules by pressure-mediated electroincorporation and electroporation: a novel method for drug and gene delivery, Bioelectrochemistry and Bioenergetics, 42: 283-292 (1997)
- Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotid artery by electroporation, Third U.S. / Japan Symposium on Drug Delivery Systems (Abstr.), 38 (1995)
- Dev, Killing cancer cells with a combination of pulsed electric fields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)
- BTX Electroporation Therapy Protocols ECT001-ECT005 (1995)
- Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)
- Sato et al., Molecular Reproduction and Development, 61: 49-56 (2002)
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