The BTX Genepaddles are paddle-style reusable electrodes suitable
for a variety of applications. Genepaddles come in two models, each model
consisting of a pair of electrodes. The Model 542 Genepaddles feature
rectangular 3 x 5mm paddles, while the Model 543 Genepaddles feature
rectangular 5 x 7mm paddles. The models vary to suit the size of the
target electroporation area. These electrodes are connected to a Model
465 Square-Post Connection Cable and interfaced with a pulse
generator via a Model 5343 Coaxial-banana Connection cable. The Genepaddles
are positioned in parallel at a predetermined gap within a tissue using the
Model 515 Genetrode Holder. Genepaddles may be cleaned with a mild detergent
and sterilized with ethanol or ethylene oxide. Properly maintained Genepaddles
have a life span of approximately 1500+ pulses, and are compatible with most
BTX Electroporation Systems.
In Vitro Embryo Gene Delivery
Genepaddles were designed for In Vitro Embryo Gene Delivery (IVEGD) in mouse embryo.
The electrodes are placed anterior and posterior to the embryo following injection
of the molecule of interest, and an electroporation pulse is delivered using a
BTX Electroporation System, such as the ECM® 830, 2001, 630, 600, and T820.
The electric field introduced by the Genepaddles causes transient pores to form
in the cells of the tissue, allowing uptake of the molecules into the cells.
Itasaki1 used a 3 x 5mm Genepaddle-style electrode with a BTX T820 to deliver
DNA to mouse embryos in vitro. His success demonstrates the potential of
electroporation to transfect different types of tissues or embryos besides
the classical avian embryo.
In Vivo Gene Delivery
Paddle-style electrodes have also been used for in vivo applications.
Miyasaka2 used an electrode of this style with a BTX T820 to deliver DNA
in vivo into the cerebral cortical anlage of E15 rat embryos. He demonstrated
high-efficiency gene transfer into intact neural tissue using electroporation.
Electroporation has the benefits of being less labor-intensive and more
economical than competitive methods for in vivo gene transfer, such as
viral methods and biolistics. Electroporation also produces greater
viability and efficiency than competitive methods for in vivo gene transfer.
|
| Standard Capabilities |
| |
Voltage Range: |
|
0 - 200 V DC |
| |
Pulse Length Range: |
|
1µsec - 99msec |
| |
Pulse Number Range: |
|
1-99 (depending on voltage and pulse length settings) |
| Physical Characteristics |
| |
|
|
Model 542 |
|
Model 543 |
|
Paddle Dimensions: |
|
3 x 5mm |
|
5 x 7mm |
|
Paddle Configuration: |
|
Rectangular |
|
Rectangular |
|
Paddle Thickness: |
|
1mm |
|
1mm |
|
Gold Plating Thickness: |
|
0.04mm |
|
0.04mm |
|
All Genepaddle electrodes are gold plated, noninvasive electrodes |
| Generator Compatibility: |
|
ECM® 830, 2001, 630, 600, T820 |
| Genetrode Holder Inter-electrode Gaps: |
|
1-10mm |
| Genepaddles |
|
Description |
|
Part Number |
| 542 |
|
3 X 5 mm Genepaddles |
|
10-002892-01 |
| 543 |
|
5 X 7 mm Genepaddles |
|
10-002896-01 |
| Accessories |
|
Description |
|
Part Number |
| 515 |
|
Genetrode Holder |
|
10-002560-01 |
| 45-0059 |
|
Enhancer 3000, Enhancer 3000 Interface Box, Oscilloscope, Communications module and cables.
|
- Itasaki et al., 'Shocking' developments in chick embryology: electroporation and in ovo gene expression, Nature Cell Biology, 1: E203-E207 (1999) BTX Reference ID#4699
- Miyasaka et al., Foreign gene expression in an organotypic culture of cortical anlage after in vivo electroporation,
NeuroReport 10: 2319-2323 (1999) BTX Reference ID#4641
|