The BTX Meander Fusion Chamber is a microslide with a novel design
specifically used for electro cell fusion. This chamber is constructed
of a conductive metal alloy, which has been deposited in a finger-like
projection array on a glass microscope slide. This method of manufacturing
creates a highly precise working area. The configuration is designed to give
direct viewing of a surface area. This can be used for viewing dimer formation
during alignment while under a microscope. The gaps are set at 0.2mm.
The Meander Fusion Chamber generates an inhomogenous field and is used for
cell fusion of microorganisms, plant, fungi, yeast and mammalian cells.
It is compatible with most BTX pulse generators. Cao1 used the Meander
Fusion Chamber to generate quadromas secreting bifunctional antibodies.
He found this method both rapid and economical to produce several quadromas
in a short time.
|
| Standard Capabilities *Depending on buffer composition and generator capability |
| |
Voltage Range: |
|
0 - 480 Vdc |
|
Frequency: |
|
1 MHz |
|
Pulse Length/Time Constants Range: |
|
1 µsec - 99 msec |
|
Pulse Number Range: |
|
1-99 (depending on voltage) |
|
Operating Temperature: |
|
5° - 40° C |
|
Intended Use: |
|
Indoor Use |
|
Relative Humidity: |
|
20-80% |
|
Maximum Altitude: |
|
2000m (6562 ft) |
|
Pollution Degree: |
|
II |
|
Insulation Category: |
|
CAT I |
| Physical Characteristics |
|
Gap size: |
|
0.2 mm |
|
Electrode Material: |
|
Silver |
|
Field Type: |
|
Inhomogeneous |
| Compatibility |
|
Generators: |
|
ECM® 630, 830, 2001, 200, 600, & T820. |
| Model |
|
Description |
|
Part Number |
| 454 |
|
Meander Fusion Chamber, pkg. of 4 |
|
01-000098-01 |
| 465 |
|
Square-post Micrograbber Cable |
|
06-700044-01 |
| 5343 |
|
Coaxial-Banana Connection Cable |
|
06-700042-01 |
| 4001 |
|
Enhancer 400 w/ Computer & Printer |
|
01-001483-01 |
- Cao et al., A rapid non-selective method to generate quadromas by microelectrofusion,
Journal of Immunological Methods, 187: 1-7 (1995). BTX Reference ID#3194.
|
)
