BTX Microslides are used primarily with the ECM® 2001 Electro Cell Manipulator.
The Microslide fits on the microscope allowing observation of the alignment
and fusion process. The Microslide 450 is composed of a glass microslide and
two strips of 0.5 mm stainless steel tubing which functions as electrodes to
provide a divergent field. These wires are set apart at different gaps to provide
various field strengths and volumes. The Micrograbber is used to connect the Microslide 450 to the Coaxial Connection
Cable, which will then be hooked up to the ECM 2001 generator. Microslide 453
has two stainless steel square bars that are set apart at various gaps to
provide homogeneous fields. The Square Post Cable is used to connect the
Microslide to the Coaxial Connection Cable, which will be hooked up to the
ECM 2001 generator.
The Microslides have been used for embryo manipulation1 and cell or protoplast
fusion2. Advantage of the Microslide is that the process of dimer formation
can be observed under the microscope.
| Standard Capabilities *Depending on buffer composition and generator capability |
|
Voltage Range: |
|
0 - 500 Vdc |
|
Pulse Length/Time Constants Range: |
|
1 µsec - 99 msec |
|
Pulse Number Range: |
|
1-99 (depending on voltage) |
|
Operating Temperature: |
|
5° - 40® C |
|
Intended Use: |
|
Indoor Use |
|
Relative Humidity: |
|
20-80% |
|
Maximum Altitude: |
|
2000m (6562 ft) |
|
Pollution Degree: |
|
II |
|
Insulation Category: |
|
CAT I |
| Physical Characteristics |
|
450 |
|
450-1 |
|
453 |
|
453-10 |
| |
Gap Size: |
|
0.5 m |
|
1.0 mm |
|
3.2 mm |
|
10 mm |
| |
Volume: |
|
20 µl |
|
40 µl |
|
0.7 ml |
|
2.2 ml |
| |
Autoclavable: |
|
No |
|
No |
|
No |
|
No |
| Compatibility |
| |
Generators |
|
ECM 630, 830, 395, 399, 2001, 600, & T820. |
| Model |
|
Description |
|
Part Number |
| 450 |
|
Microslide 20 µl - package of 10, 0.5mm gap |
|
01-000209-01 |
| 450-1 |
|
Microslide 40 µl - package of 10, 1.0mm gap |
|
01-000210-01 |
| 453 |
|
Microslide 3.2 mm gap - package of 1 |
|
01-000211-01 |
| 453-10 |
|
Microslide 10 mm gap - package of 1 |
|
01-000212-01 |
| 5343 |
|
Coaxial Banana Plug Connection Cable, 10 ft |
|
06-700042-01 |
| 465 |
|
Square Post Cables |
|
06-700044-01 |
- BTX Protocol PR0117, Personal communication with Dr. James Robl, University
of Massachusetts.
- Saunders, J.A. and Bates, G.W., Guide to Electroporation and Electrofusion,
Academic Press, p. 477-479, 1991
|
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