Description
Electric Cell-substrate Impedance Sensing (ECIS) provides a novel method to perform real time cell monitoring like never before without the use of radioactive tracers, or biological markers.  The core of ECIS technology is based on measuring the change in impedance of a small electrode to AC current flow. The heart of the measurement is a specialized slide that has 8 individual wells for cell culturing. The base of the device has an array of gold film electrodes that connect to the ECIS electronics to each of the 8 wells.

Applications

The ECIS model 800 EFM is intended for academic or industrial laboratories in the fields of tissue culture, toxicology, biotechnology, advanced biological methods, regenerative or cell biology.  ECIS utilizes measurements of impedance to monitor the following:

• Cell attachment
• Cell motility
• Membrane capacitance
• Signal transduction
• Metastatic potential of tumor cells
• Wound healing assays 

How ECIS Works

Ordering Information

Please contact BTX for pricing and ordering information.

References

1. Ren, et al. Lysophosphatidic Acid Is Constitutively Produced by Human Peritoneal Mesothelial Cells and Enhances Adhesion, Migration, and Invasion of Ovarian Cancer Cells. Cancer Res 2006; 66: (6) (2006).
2. Keese et al. "Electrical Wound-healing assay for cell in vitro". PNAS 101: 1554-1559 (2004).
3. Meyer et al.  Calcium-independent phospholipase A2 is regulated by a novel protein kinase C in human coronary artery endothelial cells, Am J Physiol Cell Physiol 288: C475-C482, (2005).
4. Charrier, et al.  ADAM-15 inhibits wound healing in human intestinal epithelial cell monolayers." AJP Gastrointest Liver Physiol. 288:346-353, (2005).
5. McLaughlin et al.  Functional Selectivity of G Protein Signaling by Agonist Peptides and Thrombin for the Protease-activated Receptor-1. J. Biol. Chem. 280(26): 25048-25059 (2005).