Cell biology research frequently needs the ability to transfect hundreds if not thousands of samples at once. Using traditional electroporation techniques, such as cuvettes, would be time-consuming and expensive, if not impossible. High throughput electroporation allows the user to accelerate research by conducting multiple experiments at one time on a single plate.
Advanced Transfection and Transformation
When a large number of samples need to be transfected, minimizing the number of separate transfer steps saves time and work and therefore ultimately money. In the quest for more efficient methods, assay systems are being miniaturized to allow the handling of more samples in 96 or 25 well formats.
High throughput electroporation applications in transfection include genomic library construction, delivery of siRNA, genome sequencing studies, subcloning, mutagenesis, and directed evolution studies.
High Throughput Screening for Target ID and Validation
Biomolecular screening is one of the principal tools used for the discovery of new drugs in the biopharmaceutical industry today. Batches of compounds are tested for binding activity or biological activity against target molecules. High throughput screening seeks to screen large numbers of compounds rapidly and in parallel.
Electroporation transfection applications in this context include antisense oligonucleotide delivery and siRNA delivery to cells for identification and validation of potential targets.
Genomics and Proteomics
Transfection is a valuable way to investigate gene function in cells. Recent advances in genomics, the study of genes and their function, are bringing about a revolution in our understanding of the molecular mechanisms of disease, including the complex interplay of genetic and environmental factors.
Genomics is also stimulating the discovery of breakthrough healthcare products by revealing thousands of new biological targets for the development of drugs, and by giving scientists innovative ways to design new drugs, vaccines, and DNA diagnostics. Sequencing the genome established a firm foundation-but proteomics, the study of the full set of proteins encoded by a genome, is the next, more challenging phase in the process of understanding cellular biochemistry and mechanisms of disease. High throughput electroporation will facilitate this exciting area of research.
| Standard Capabilities |
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Interface: |
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Digital User Interface |
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Input: |
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110 V/220 V Universal |
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Charge Time: |
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13 sec maximum |
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Voltage Range: |
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5 - 500 V LV Mode/ 1 V resolution
505 - 3000 V HV Mode/ 5 V resolution
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Pulse Length Range: |
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1 ms - 999 ms LV Mode/ 1 ms resolution
10 µsec - 999µsec LV Mode/ 1 µsec resolution
1 sec - 10 sec LV Mode/ 0.1 sec resolution
10 µs - 600 µs HV Mode/ 1 µs resolution
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Multiple Pulsing: |
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1 - 99 pulses |
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Pulse Interval: |
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100 msec - 10 sec |
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Programmability: |
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Storage for 3 setups (V, t, n, interval) |
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Arc Control: |
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Arc Quenching |
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| Other Electrical Characteristics |
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Capacitance: |
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4000 µF LV, 110 µF HV |
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Amperage: |
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500 A LV, 100 A HV |
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| Physical Characteristics |
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Footprint: |
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12.5" x 12.25" x 5.5" (14 x 31.8 x 31.1 cm) (W x D x H) |
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Weight: |
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15 lbs. (6.8 kg) |
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Display: |
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20 x 4 character LCD |
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Controls: |
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Single Rotary Encoder with push button toggle between all set parameters.
Additional on/off Power and Start switches |
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| Monitoring: |
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Displays all set parameters after pulsing |
| Remote Operation: |
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Footswitch available. Please contact BTX Technical Services at 1-800-272-2775, 508-893-8999 or btxinfo@harvardapparatus.com for assistance
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System
| Catalog # |
Model |
|
Available Configurations |
| 45-0002 |
8300 |
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ECM 830 Electroporation System Includes:
ECM 830 Generator with Power Supply
Model 630 B Safety Stand
Model 610, 620, 640 Disposable Electroporation Cuvettes Plus™ 10ea.
Model 660 Cuvette Rack
Manuals and Instruction Sheet |
| 45-0421 |
HT-96/200-830 |
|
HT-96/200 Advanced Electroporation System Includes:
ECM 830 Generator
2 x 96-Well
4 mm Gap Plates
HT-200 Plate Handler |
| 45-0411 |
HT-25/200-830 |
|
HT-25/200 System Includes:
ECM 830 Generator
6 x 25-Well
4 mm Gap Plates
HT-200 Plate Hanlder |
| Catalog # |
Model |
|
Description |
| 45-0401 |
HT-200 |
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96 Well Plate Handler with Automatic Column Switching. Includes connectors for standard pulse generators and pliers for adjusting from 96 to 25 Well configuration |
| 45-0400 |
HT-100 |
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96 Well Plate Handler with Manual Column Switching. Includes connectors for standard pulse generators and pliers for adjusting from 96 to 25 Well configuration |
| 45-0452 |
HT-P96-4 |
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96 Well Electroporation Plate - 4 mm gap |
| 45-0462 |
HT-P25-4 |
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25 Well Electroporation Optimizer Plate - 4 mm gap |
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