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 Efficient Electroporation Transfection
High efficiency transfection of cell lines and previously considered “hard to transfect” cells
High Cell Viability
Low toxicity resulting in improved cell viability
Single Buffer for all Cell Types
One buffer used in place of standard
electroporation buffers for all mammalian
cell types.
The BTXpress™ is the New solution in High Performance Electroporation. A single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered “hard to transfect” by chemical and other non-viral methods.
This solution in combination with the BTX electroporation instruments, provides researchers with the versatility and success needed across a broad range of cell types while maintaining critical cell viability. Transfection using this high performance electroproation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.
BTXpress™ solution is the first electroporation reagent that meets all of your high performance transfection needs without sacrificing control over your experiment or your budget.
The BTXpress™ solution offers increased numbers of transfections per kit compared to our competitors providing better value to the researcher. As a universal solution the BTXpress™ electroporation reagent can be used with electroporation generators, including Amaxa™ Nucleofector® to deliver similar to equal performance without the cost associated with other systems. The BTXpress™ High Performance Electroporation Solution are offered as kits which includes the BTX Plus cuvettes with transfer pipettes or as a buffer alone.
 Click on the photo above for a larger image
Table 1: BTXpress™ “High Peformance Electroporation Solution" vs. Amaxa™ Nucleofector®. Cells were electroporated with an EGFP reporter vector in parallel, using the BTX ECM 830 Square Wave Electroporator with the BTXpress™ High Performance Electroporation Solution or using the Amaxa™ (Lonza) system. EGFP expressing cells were identified 24hrs post-electroporation by flow cytometery and presented as a percentage of the live cell population.
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 Click on the photo above for a larger image
Table 2: BTXpress™ Solution Transfection Efficiency vs. Amaxa™: Cells were electroporated in parallel with an EGFP reporter vector using either the BTX electroporation system with BTXpress™ High Performance Electroporation Solution or PBS. In comparison to the same cells transfected in the Amaxa™ (Lonza) system using the Amaxa™ kit V solution. The EGFP expressing cells were identified 24hrs post-electroporation by flow cytometery and presented as a percentage of the live cell population.
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Read this document before you begin with your experiment
| Kit Part Numbers |
2mm Gap Cuvette includes transfer pipette (Blue Cap) |
4mm Gap Cuvettes include transfer pipettes (Yellow cap) |
BTXpress™ Electroporation Solution |
| 45-0800 |
5 |
5 |
2mls |
| 45-0803 |
50 |
NA |
5.0mls |
| 45-0804 |
NA |
20 |
5.0mls |
| 45-0806 |
100 |
NA |
10.0mls |
| 45-0807 |
NA |
40 |
10.0mls |
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| Reagent Only Part Numbers |
No Cuvettes |
No Cuvettes |
BTXpress™ Electroporation Solution |
| 45-0802 |
NA |
NA |
5.0mls |
| 45-0805 |
NA |
NA |
10.0mls |
Kits:
| Catalog # |
|
Description |
| 45-0803 |
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BTXpress™ Solution Kit 50 Reactions in
2mm gap Cuvettes: Includes 5.0ml bottle BTXpress™
solution and 5 bags of (10cuvettes per bag), 2mm gap
BTXplus cuvettes with transfer pipette.
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| 45-0804 |
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BTXpress™ Solution Kit 40 Reactions in 4mm gap
Cuvettes: Includes 5.0ml bottle BTXpress™ solution and
2 bags of (10cuvettes per bag) 4mm gap BTXplus
cuvettes with transfer pipette.
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| 45-0806 |
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BTXpress™ Solution Kit 100 Reactions in 2mm gap
Cuvettes: Includes a 10ml bottle of BTXpress™ solution
and 2 bags of 50 cuvettes per bag, 2mm gap BTXplus
cuvettes with transfer pipette.
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| 45-0807 |
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BTXpress™ Solution Kit 40 Reactions in 4mm gap
Cuvettes: Includes a 10ml bottle of BTXpress™ solution
and 4 bags of (10 cuvettes per bag) 4mm gap BTXplus
cuvettes with transfer pipette
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Solution:
| Catalog # |
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Description |
| 45-0802 |
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BTXpress™ Solution: 5.0ml bottle for up to 50 reactions
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| 45-0805 |
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BTXpress™ Solution: 10ml bottle for up to 100 reactions
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