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APPLICATIONS
The ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo electroporation applications. The versatility of the ECM® 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo and ex vivo tissues, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM® 830 can be used in combination with a wide array of BTX® specialty electrodes and accessories to enhance your molecular and drug delivery experiments.
96-WELL ELECTROPORATION
The High Throughput (HT) 96- & 25-well system offers the advantage of multi-well technology. This permits a large number of samples to be quickly processed using high throughput plate handler technology.
MONITORING OPTION
The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment. This data can be stored on a memory stick or downloaded to a computer for potential analysis, documentation and validation purposes.
COMBINATION SYSTEMS
The combo system includes the power and flexibility of the ECM 630 exponential decay wave generator to provide the highest transformation efficiencies for a wide range of bacteria and yeast strains. BTX offers the ECM 630/ECM 830 combo system complete with two safety stands and sample cuvettes.
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Standard Capabilities |
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| Operational Status | Internal self test upon start-up |
| Interface | Digital User Interface |
| Input | 110 V/220 V Universal |
| Charge Time | 5 sec maximum (without delay) |
| Pulse Length |
Range 10 μs – 999 μs LV Mode/ 1 μs resolution
1 msec – 999 msec LV Mode/ 1 msec
resolution 1 sec – 10 sec LV Mode/
0.1 sec resolution 10 μs – 600 μs HV
Mode/ 1 μs resolution
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| Voltage Range |
5 – 500 V LV Mode/ 1 V resolution 505 –
3000 V HV Mode/ 5 V resolution
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| Multiple Pulsing | 1 – 99 |
| Pulse Interval | 100 msec – 10 sec |
| Programmability | Storage for 3 protocol setups (V,t,n,interval) |
| Arc Control | Arc Quenching™ |
| Safety | Generator short circuit proof |
| Capacitance | 4000 μF LV, 111 μF HV |
| Input | 500 A limit at 100 μs |
| Remote Operation | Footswitch available |
| Order # | Product |
| 45-0002 |
ECM® 830 Electroporation System includes, ECM® 830
Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm,
4 mm pkg. of 30 (10 each) and Cuvette Rack 660
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| 45-0052 | ECM® 830 Generator Only |
| 45-0421 | HT 96/200 includes ECM 830 Generator, 2- x 96-Well
Plates (4 mm), Plate Seals and HT-200 Plate Handler |
| 45-0411 | HT 25/200 includes ECM® 830 Generator, 6- x 25-Well
Plates (4 mm), Plate Seals and HT-200 Plate Handler |
| 45-0061 |
ECM® 830 / ECM® 630 Combination Package includes
ECM® 830 Generator, ECM® 630 Generator, 2 x Safety
Stands, Sample Package of Cuvettes and Cuvette Rack
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| 45-0060 |
ECM® 830 / ECM® 399 Combination Package includes
ECM® 830 Generator, ECM® 399 Generator, Safety
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| 45-0072 |
ECM® 830 / ENHANCER 3000 System:
ENHANCER 3000 Probe, ENHANCER Interface Box,
Oscilloscope, Communications Module, ECM® 830
Generator, Safety Stand, 30 Cuvettes (10 each 1 mm,
2 mm and 4 mm) and Cables
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BTX Monthly Tech Trends Articles
For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor
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