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ECM® 830 Square Wave Electroporation System

  • Image 1
  • Safety Stand, Cuvettes & Cuvette Rack
  • ECM 830 Electroporator



FEATURES

  • A wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 μsec to 10 sec
  • Arc Quenching
  • Digital display of actual voltage and pulse
  • length delivered

 

APPLICATIONS

  • Mammalian Cells Transfection
  • In Vitro, In Vivo, Ex Vivo & In Ovo Tissue Transfection
  • Nuclear Transfer
  • Plant Protoplast Transfection
  • High Throughput 25/96 Well Electroporation

 

The ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo electroporation applications. The versatility of the ECM® 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo and ex vivo tissues, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM® 830 can be used in combination with a wide array of BTX® specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

96-WELL ELECTROPORATION

The High Throughput (HT) 96- & 25-well system offers the advantage of multi-well technology. This permits a large number of samples to be quickly processed using high throughput plate handler technology.

MONITORING OPTION

The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment. This data can be stored on a memory stick or downloaded to a computer for potential analysis, documentation and validation purposes.

COMBINATION SYSTEMS

The combo system includes the power and flexibility of the ECM 630 exponential decay wave generator to provide the highest transformation efficiencies for a wide range of bacteria and yeast strains. BTX offers the ECM 630/ECM 830 combo system complete with two safety stands and sample cuvettes.

Standard Capabilities

 
Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V/220 V Universal
Charge Time 5 sec maximum (without delay) 
Pulse Length
Range 10 μs – 999 μs LV Mode/ 1 μs resolution
1 msec – 999 msec LV Mode/ 1 msec
resolution 1 sec – 10 sec LV Mode/
0.1 sec resolution 10 μs – 600 μs HV
Mode/ 1 μs resolution
Voltage Range
5 – 500 V LV Mode/ 1 V resolution 505 –
3000 V HV Mode/ 5 V resolution
Multiple Pulsing  1 – 99
Pulse Interval 100 msec – 10 sec 
Programmability  Storage for 3 protocol setups (V,t,n,interval)
Arc Control Arc Quenching™
Safety Generator short circuit proof
Capacitance 4000 μF LV, 111 μF HV
Input 500 A limit at 100 μs
Remote Operation Footswitch available
Order # Product
45-0002
ECM® 830 Electroporation System includes, ECM® 830
Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm,
4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0052 ECM® 830 Generator Only 
45-0421 HT 96/200 includes ECM 830 Generator, 2- x 96-Well

Plates (4 mm), Plate Seals and HT-200 Plate Handler

 
45-0411  HT 25/200 includes ECM® 830 Generator, 6- x 25-Well

Plates (4 mm), Plate Seals and HT-200 Plate Handler

 
45-0061 
ECM® 830 / ECM® 630 Combination Package includes
ECM® 830 Generator, ECM® 630 Generator, 2 x Safety
Stands, Sample Package of Cuvettes and Cuvette Rack
 
45-0060 
ECM® 830 / ECM® 399 Combination Package includes
ECM® 830 Generator, ECM® 399 Generator, Safety 
Stand,PEP, Sample Package of Cuvettes and Cuvette Rack
 
45-0072 
ECM® 830 / ENHANCER 3000 System:
ENHANCER 3000 Probe, ENHANCER Interface Box,
Oscilloscope, Communications Module, ECM® 830
Generator, Safety Stand, 30 Cuvettes (10 each 1 mm,
2 mm and 4 mm) and Cables

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road

Holliston, MA 01746

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent

integration of small hairpin RNAs in repopulating spermatogonial cells in

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Clape, C. et al., miR-143 Interferes with ERK5 Signaling, and Abrogates

Prostate Cancer Progression in Mice, 2009, PLOS ONE

 

Nyugen, K. et al., T Cell Costimulation via the Integrin VLA-4 Inhibits the

Actin-Dependent Centralization of Signaling Microclusters Containing the

Adaptor SLP-76, 2008, Immunity

 

Lin, G. et. al, Regeneration of neural crest derivatives in the Xenopus

tadpole tail, 2007, BMC Developmental Biology

 

Chalberg, T. et al., C31 Integrase Confers Genomic Integration and Long-

Term Transgene Expression in Rat Retina, June 2005, Investigative Ophthalmology &

Visual Science, Vol. 46, No. 6

 

Deora, A. et al., Efficient Electroporation of DNA and Protein into

Confluent and Differentiated Epithelial Cells in Culture, 2007, Traffic



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