Loading... Please wait...This electrode is designed for delicate and/or difficult tissue transfection. Ex vivo electroporation is an efficient, effective method to introduce genes, drugs or any number of molecules into ex-plant tissues. A common application is mouse brain slice for studying neuronal development. This specialty electrode makes transfection quick and simple and is compatible with both the BTX ECM 830 and ECM 2001 generator. The electrode is compromised of two parts, the glass petri dish electrode and electrode wand. The glass petri dish contains a square shaped platinum electrode chamber to secure the tissue. The wand incorporates an identical shaped platinum electrode, which is placed over the chamber to complete electroporation. This sandwich configuration ensures a homogeneous field of energy for optimum transfection.
| Generator Compatibility | ECM 830 and ECM 2001 |
| Voltage Range | 0 – 100 Volts |
| Pulse Length | 10 μsec – 100 msec |
| Chamber Depth | 1mm |
| Electrode Material | Platinum |
| Wand Material | Platinum |
| Dimensions: | |
| Dish Electrode 7mm | Length 7mm x Width 7mm x Depth 1mm |
| Wand Electrode 7mm | Length 7mm x Width 7mm |
| Dish Electrode 10mm | Length 10mm x Width 10mm x Depth 1mm |
| Wand Electrode 10mm | Length 10mm x Width 10mm |
| Kits | |
| Item # | Description |
| 45-0500 | Tissue Slice Platinum Chamber Electrode Kit, 10mm Includes: Petri Dish Platinum Electrode Chamber, 10mm and a Platinum Wand Electrode, 10mm, with micrograbber cable and single adaptor cable. |
| 45-0490 | Tissue Slice Platinum Chamber Electrode Kit, 7mm Includes: Petri Dish Platinum Electrode Chamber, 7mm and a Platinum Wand Electrode,7mm, with micrograbber cable and single adaptor cable. |
Tissue Slice Chambers
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| 45-0491* | Petri Dish Platinum Tissue Slice Electrode Chamber Only, 7mm |
| 45-0501* | Petri Dish Platinum Tissue Slice Electrode Chamber Only, 10mm |
Wand Electrodes
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| 45-0492** | Platinum Electrode Wand Only, 7mm |
| 45-0502** | Platinum Electrode Wand Only, 10mm |
| Cables | |
| 45-0503 | Micro-Grabber Cables for Tissue Slice Chamber |
| 45-0511 | Single Adaptor Cable for Wand Electrode |
| * Requires Platinum Electrode Wand either 45-0492 or 45-0502 and cables 45-0503 and 45-0511 |
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| ** Requires Platinum Tissue Slice Chamber either 45-0491 or 45-0501 and cables 45-0503 and 45-0511 | |
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For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor