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MicroJect 1000A

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APPLICATIONS

  • Nuclear Transfer Applications
  • Transgenic Animal Development
  • Injection of Mouse, Xenopus, Zebrafish and other oocytes
  • Intra-cytoplasmic sperm injection
  • Cell injection
  • Extracellular brain injections
  • Injection of DNA, mRNA, microbeads and proteins


The MicroJect 1000A MAX (MJ 1000A) is the newest addition to the family of BTX transfection products. The MJ 1000A MAX offers an alternative method to deliver genes, proteins, macromolecules and micro-beads by direct injection into cells, tissues and oocytes simply and efficiently. This microinjection system provides a reliable, consistent and precise delivery of volumes through digitally-controlled, stable pressure regulation which is maintained for a set duration of time. The compressed gas, internally-controlled pressure system also allows the precise delivery of desired volumes ranging from femtoliters to microliter volumes. The MJ 1000A MAX is capable of holding a cell, oocyte or early stage embryo stationary while simultaneously using a separate pressure channel for injections. The system is versatile enough to provide the same consistent performance needed to inject large volumes into tissue such as capillaries, or pico volumes, for nuclear injections. It is also ideal for the gentle transfer of delicate fetal or stem cells into oocytes.

PRESSURE CONTROL FEATURES

Fill/Clear/Hold
The MJ 1000A MAX provides key pressure features designed to maximize your injection potential with two negative and three positive pneumatic capabilities. The negative pressure feature, or vacuum function, allows researchers the ability to fill the micropipettes from their tips. The “Fill” feature reduces waste of valuable injection material. The “Hold” feature provides the means to immobilize and manipulate a cell or oocyte using a micropipette. The positive pressure feature allows for the precise discharge of fluids by simply using the “Clear” function of the system.

Balance Pressure
It’s all about the balance of pressure with our MJ 1000A MAX system. The unique “Balance” feature provides a secondary balance pressure to maintain positive pressure on the injection pipette. This is important before and after injections to avoid the chance of diluting a sample due to capillary action. It also prevents clogging of the injection needle.

Accessories
The MJ 1000A MAX system provides the “MAX”imum potential for your microinjection applications. The durability and precision of the MJ 1000A is coupled with two footswitches for easy operation of the Clear/Fill features. The system also includes two pipette holders and the appropriate adaptors.

 

Input Gas Pressure:

 

70 to 105 p.s.i. (480 to 720 kPa)

Injection Pressure:

 

0.2 to 60 p.s.i. (413 kPa), regulated, multi-turn control

Balance Pressure:

 

0.1 to 3.5 p.s.i. (68.9 kPa), regulated, multi-turn control, other ranges available upon request

Fill Vacuum:

 

Internally produced, -12.0 p.s.i. (-82 kPa), unregulated

Holding Vacuum:

 

Internally produced, 0 to 3 in H2O (0 to 0.75 kPa or 0 to 0.1 p.s.i.), regulated

Clearing Pressure:

 

Input gas pressure, unregulated

Injection Timer:

 

0.01 to 0.99 sec in 10 msec steps; 1 to 99 sec in Pulse Width 1 sec steps

Injection Count Display:

 

Digital, 0 through 9999

Duration Mode:

 

Internally timed or externally gated

Time Trigger:

 

Front panel, foot switch, or external TTL pulse (BNC)

Pressure Units:

 

p.s.i./kPa; switch selectable

Pressure Monitor:

 

BNC connector, 10 mV/p.s.i.

Pressure Readout:

 

Inject, balance, clear, output port

Line Voltage:

 

100/110/220/240 VAC

Power Usage:

 

220 W

Meter Accuracy:

 

0.1% full scale

Foot Switches:

 

Inject, fill, hold, and gated; provided in Plus and Deluxe pkgs.

Weight:

 

6.8 kg (15 lb)

Dimensions H x W x D:

 

11 x 38 x 25.5 cm (5 x 15 x 10 in)

Accessories Supplied:

 

Input, output and holding hoses

Catalog Number

 

Description

45-0760

 

MicroJect 1000A Max System includes: MicroJect 1000A pico-injector with Injection, Balance, Clear/Fill and Hold pressures. Also included are two Footswitches, Input/Output Hoses, Holding Hose, two Pipette Holders and Input Adaptor for Hoses, Power Cord and Manual.

45-0759

 

MicroJect 1000A Plus System; MicroJect 1000A pico-injector with Injection, Balance, Clear/Fill and Hold Pressure. Includes one Footswitch, Input/Output Hoses, Holding Hose, one Pipette Holder and Input Adaptor for Hoses, Power Cord and Manual.

45-0758

 

MicroJect 1000A Basic System: MicroJect 1000A pico-injector with Injection, Balance, Clear/Fill and Hold Pressure, Power Cord and Manual.

This product meets the requirements of the European Communities (EC), and complies with EMC Directive 89/336/EEC and 73/23/EEC for product safety.

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

Technical Specifications

User's Manual

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

BTX-Division of Harvard Apparatus

84 October Hill Road 

Holliston, MA 01746 

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Not available.


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