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- Meander Fusion Chamber
Meander Fusion Chamber
The BTX Meander Fusion Chamber is a novel microslide design which is specifically used for electro cell fusion. The Meander Fusion Chamber generates a divergent field and is used for fusion of mammalian cells, plant, yeast, fungi and microorganisms. This specialty electrode is constructed of a conductive metal alloy. It has two primary bars that are connected by many tiny fingerlike projections. These projections are spaced 0.2 mm apart. This electrode is mounted on a glass slide. It is designed for direct viewing of dimer formation during alignment while under a microscope.
APPLICATIONS
• Cell Fusion
• Plant Protoplast Fusion
The Meander Fusion Chamber generates an inhomogenous field and is used for cell fusion of microorganisms, plant, fungi, yeast and mammalian cells. It is compatible with the ECM 830 or ECM 2001 pulse generators. Cao1 used the Meander Fusion Chamber to generate quadromas secreting bifunctional antibodies. He found this method both rapid and economical to produce several quadromas in a short time.
| Generator Compatibility | ECM 2001 |
| Field Type: | Divergent |
| Max Voltage: |
|
| AC |
16 V (0 – peak) |
| DC | 0 - 480 V |
| Pulse length | 1 usec-99 msec |
| Number of pulses | 1-99 (depending on voltage) |
| Gap size | 0.2 mm |
| Autoclavable | No |
| Item |
Description | Gap size | Package size | |
| 45-0107*† | Meander Fusion Chamber* |
0.2 mm | pkg. of 4 (Model 454) | |
| 45-0216 | Micrograbber to Banana Connection Cables. Set of one black and red cable | |||
| 45-0088 | Female to female connection cables to connect with cable 45-0216 in ECM 2001 systems | |||
| 45-0087 | Micrograbber adaptor connector for use with 45-0083 cables in the ECM 2001 system | |||
| * Requires 45-0216 Micrograbber to Banana Plug Cable | ||||
| † Requires 45-0087 Adapter Set for Connection to EMC 2001 | ||||
1. Cao et al., A rapid non-selective method to generate quadromas by microelectrofusion, Journal of Immunological Methods, 187: 1-7 (1995). BTX Reference ID#3194.
2. Von Keller et al., Intracelluar movements and reorganization of electrically fused sunflower protoplasts, Int. J. Plant sci: 156(6): 764-773 (1995)
BTX Monthly Tech Trends Articles
For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor
