electrofusion hybridoma large volume cell fusion Hybrimune

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Hybrimune System for Volumes up to 9mls

Hybrimune System for Volumes up to 9mls

The Hybrimune™ System is an advanced electrofusion solution for fast, efficient cell fusion in hybridoma production, hybrid cell formation or dendritic-tumor cell fusions. The Hybrimune™ system includes an innovative fusion chamber design, proprietary Cytofusion® medium and sophisticated, tri-phasic electric field pulses that quickly position cells and disrupt cell membranes for maximum cell fusion efficiency with short cycle-times and minimal heating or turbulence for excellent cell viability.

The Hybrimune boasts a gradual increase in AC amplitude to compress the cells for maximal cell-cell contact, then the DC pulse is applied and the researcher has the option of doing multiple pulses in different voltage and duration if required, then a final AC waveform holds the cells in place and stabilizes the fusion as the force is gradually reduced. The waveform generator is fully-programmable for pulse parameter optimization to maximize efficiency and cell viability. A computer is required for the application software but is not included in the system.

Use of the AgilePulse HT system (formerly Cyto LVT-P) is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Hybrimune^® device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Agile Pulse In Vivo system (formerly the DermaVax device) is not authorized for any veterinary and human therapeutic uses. Contact Cellectis therapeutics contact@cellectis-therapeutics.com for such uses.

Specifications
Ordering Info
Technical
References

WAVEFORM SPECIFICATIONS
The Hybrimune™ Waveform Generator is programmed using the interface software. The Hybrimune™ waveform generator interface software must be installed on a local computer with these minimum specifications: Windows® 98 or higher, 2Mb free disk space, and at least one RS232 Serial Port. If only USB ports exist, then a USB-to-Serial adapter will be required, and should be installed first.
The following parameters are available:

	Pulse Function		Constant, linear, non-linear
	Pulse Amplitude		100-1000 V
	Pulse Width Range		20 – 1000 ms
	AC Start Peak Range		5-75 Vs
	AC Stop Peak Range		5-75 V
	AC Frequency		0.2 to 2.0 MHz
	AC Duration		0 to 60 sec

FUSION CHAMBER SPECIFICATIONS
The optimization and production chambers have been engineered to have identical electrical characteristics to facilitate direct scale-up to production once pulse parameters have been optimized. In addition the small chamber has a transparent bottom to permit visualization of the cell alignment by inverted or regular microscope.

Parameter	Optimization Chamber	Production Chamber
Volume	2 ml	9 ml
Outer ID	45.72 mm	45.72 mm
Inner OD	38.10 mm	38.10 mm
Gap	3.81 mm	3.81 mm
Well Height	5 mm	18 mm
Inner/Outer Radius	0.8333	0.8333

For reuse, the fusion chamber can be cleaned with NaOH, sterilization by EtOH, and Spor-Klenz® for spores and mycoplasma.

Catalog		Description
47-0300N		Hybrimune System Includes: Hybrimune waveform generator, 2ml and 9ml coaxial chambers, BTXpress Cytofusion Medium C, user interface software, cables and manual. Requires Windows based laptop or PC (not included).
Accessories
47-0020		9ml Coaxial chamber for production

47-0030 47-0302		2ml Coaxial chamber for optimization Chamber Cable

47-0301		User Interface Application Software

47-0001		BTXpress Cytofusion Medium C, 500ml volume