electrofusion hybridoma large volume cell fusion Hybrimune

Hybrimune™ Hybridoma Production System

The BTX Hybrimune™ System is an advanced electrofusion system for fast, efficient cell fusion in hybridoma production, hybrid cell formation or nuclear transfer applications. The Hybrimune™ system includes an innovative fusion chamber design, proprietary BTXpress Cytofusion^® medium and sophisticated, tri-phasic electric field pulses that quickly position cells and disrupt cell membranes for maximum cell fusion efficiency with short cycle-times and minimal heating or turbulence for excellent cell viability. In addition, Hybrimune™ delivers a cell alignment waveform which is a patented sequence that enhances hybridoma yield. Compared to standard PEG fusion, yield improvements of 10 fold or more observed. The Hybrimune™system consists of a user-friendly, programmable waveform generator controlled through the User-Interface Application Software running on a Windows-based computer system (not included). The fusion chamber uses coaxial electrodes designed for optimal electric field stimulation, independent of bath height. In this way, pulse parameters defined with the low volume optimization chamber are directly applicable for the large volume production chamber. The optimization chamber includes a transparent bottom for microscope viewing during the process optimization. BTXpress Cytofusion^® medium is a specially formulated, low conductivity solution for robust cell fusion efficiency.

Applications

Hybridoma production
Dendritic and tumor cell fusion
Nuclear Transfer

Use of the AgilePulse HT system (formerly Cyto LVT-P) is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Hybrimune^® device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Agile Pulse In Vivo system (formerly the DermaVax device) is not authorized for any veterinary and human therapeutic uses. Contact Cellectis therapeutics contact@cellectis-therapeutics.com for such uses.

Specifications
Ordering Info
Technical
References

WAVEFORM SPECIFICATIONS
The Hybrimune™ Waveform Generator is programmed using the Application Software.
The following parameters are available:

	Pulse Function		Constant, linear, non-linear
	Pulse Amplitude		100-1000 V
	Pulse Width Range		20 – 1000 ms
	AC Start Peak Range		5-75 Vs
	AC Stop Peak Range		5-75 V
	AC Frequency		0.2 to 2.0 MHz
	AC Duration		0 to 60 sec

FUSION CHAMBER SPECIFICATIONS
The optimization and production chambers have been engineered to have identical electrical characteristics to facilitate direct scale-up to production once pulse parameters have been optimized. In addition the small chamber has a transparent bottom to permit visualization of the cell alignment by inverted or regular microscope.

Parameter	Optimization Chamber	Production Chamber
Volume	2 ml	9 ml
Outer ID	45.72 mm	45.72 mm
Inner OD	38.10 mm	38.10 mm
Gap	3.81 mm	3.81 mm
Well Height	5 mm	18 mm
Inner/Outer Radius	0.8333	0.8333

For reuse, the fusion chamber can be cleaned with NaOH, sterilization by EtOH, and Spor-Klenz® for spores and mycoplasma.

Catalog		Description
47-0300		Hybrimune System Includes: Hybrimune waveform generator, 2ml and 9ml coaxial chambers, BTXpress Cytofusion Medium C, user interface software, cables and manual. Requires Windows based laptop or PC (not included).
Accessories
47-0020		9ml Coaxial chamber for production

47-0030		2ml Coaxial chamber for optimization

47-0301		User Interface Application Software

47-0001		BTXpress Cytofusion Medium C, 500ml volume