The Hybrimune™ Hybridoma Production System is an advanced electrofusion system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and dendritic-tumor cell fusions.
Yield improvements of 10 fold or more compared to standard PEG fusion
The Hybrimune system includes an innovative fusion chamber design, proprietary BTXpress Cytofusion® medium and sophisticated, tri-phasic electric field pulses that quickly position cells and disrupt cell membranes for maximum cell fusion efficiency with short cycle-times and minimal heating or turbulence for excellent cell viability. In addition, Hybrimune delivers a cell alignment waveform which is a patented sequence that enhances hybridoma yield.
The Hybrimune system consists of a user-friendly, programmable waveform generator controlled through the User-Interface Application Software running on a Windows-based computer system (not included). The fusion chamber uses coaxial electrodes designed for optimal electric field stimulation, independent of bath height. In this way, pulse parameters defined with the low volume optimization chamber are directly applicable for the large volume production chamber. The optimization chamber includes a transparent bottom for microscope viewing during the process optimization. BTXpress Cytofusion medium is a specially formulated, low conductivity solution for robust cell fusion efficiency.
The Hybrimune boasts a gradual increase in AC amplitude to compress the cells for maximal cell-cell contact. The DC pulse is then applied. The researcher has the option of doing multiple pulses of different voltages and duration if required. A final AC waveform holds the cells in place and stabilizes the fusion as the force is gradually reduced. The waveform generator is fully-programmable for pulse parameter optimization to maximize efficiency and cell viability.
A computer is required for the application software but is not included in the system.
The Hybrimune is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
|Item #||Name||Included Items|
|47-0300N||Hybrimune Electrofusion System (N. America)||Hybrimune waveform generator, 2 ml and 9 ml coaxial chambers, BTXpress Cytofusion Medium C, user interface software, cables and manual. Requires Windows based laptop or PC (not included)|
|47-0300NINT||Hybrimune Electrofusion System||Hybrimune waveform generator, 2 ml and 9 ml coaxial chambers, BTXpress Cytofusion Medium C, user interface software, cables and manual. Requires Windows based laptop or PC (not included)|
|47-0305N||Hybrimune Electrofusion Generator Only (N. America)||Hybrimune Electrofusion Generator only|
Use of the Hybrimune device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis at email@example.com for further details.
The Hybrimune™ Waveform Generator is programmed using the Application Software. The following parameters are available:
|Pulse Function||Constant, linear, non-linear|
|Pulse Amplitude||100 - 1000 V|
|Pulse Width Range||20 – 1000 ms|
|AC Start Peak Range||5 - 75 Vs|
|AC Stop Peak Range||5 - 75 V|
|AC Frequency||0.2 to 2.0 MHz|
|AC Duration||0 to 60 sec|
FUSION CHAMBER SPECIFICATIONS
The optimization and production chambers have been engineered to have identical electrical characteristics to facilitate direct scale-up to production once pulse parameters have been optimized. In addition the small chamber has a transparent bottom to permit visualization of the cell alignment by inverted or regular microscope.
|Parameters||Optimization Chamber||Production Chamber|
|Volume||2 ml||9 ml|
|Outer ID||45.72 mm||45.72 mm|
|Inner OD||38.10 mm||38.10 mm|
|Gap||3.81 mm||3.81 mm|
|Well Height||5 mm||18 mm|
- Hybridoma production
- Hybrid cell formation
- Dendritic-tumor cell fusions
Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1. Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC. J Biol Chem. 2011 May 27;286(21):18969-81
An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies. Yu X, McGraw PA, House FS, Crowe JE Jr. J Immunol Methods. 2008 Jul 31;336(2):142-51.
Human antibodies for immunotherapy development generated via a human B cell hybridoma technology. Li J, Sai T, Berger M, Chao Q, Davidson D, Deshmukh G, Drozdowski B, Ebel W, Harley S, Henry M, Jacob S, Kline B, Lazo E, Rotella F, Routhier E, Rudolph K, Sage J, Simon P, Yao J, Zhou Y, Kavuru M, Bonfield T, Thomassen MJ, Sass PM, Nicolaides NC, Grasso L. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3557-62.
Generation of dendritic cell-tumor cell hybrids by electrofusion for clinical vaccine application. Trevor KT, Cover C, Ruiz YW, Akporiaye ET, Hersh EM, Landais D, Taylor RR, King AD, Walters RE. Cancer Immunol Immunother. 2004 Aug;53(8):705-14.